Randall, D J Wilson, J M Peng, K W Kok, T W Kuah, S S Chew, S F Lam, T J Ip, Y K
Published in
The American journal of physiology
Periophthalmodon schlosseri can maintain ammonia excretion rates and low levels of ammonia in its tissues when exposed to 8 and 30 mM NH4Cl, but tissue ammonia levels rise when the fish is exposed to 100 mM NH4Cl in 50% seawater. Because the transepithelial potential is not high enough to maintain the NH4+ concentration gradient between blood and w...
Cao, H J Smith, T J
Published in
The American journal of physiology
Human orbital fibroblasts from patients with severe thyroid-associated ophthalmopathy are particularly susceptible to the actions of a variety of proinflammatory molecules. In this study, we demonstrate that the inductions of prostaglandin endoperoxide H synthase-2 (PGHS-2), interleukin (IL)-1alpha, and IL-1beta by leukoregulin, a product of activa...
Lloyd, P G Hardin, C D
Published in
The American journal of physiology
We used (13)C-labeled substrates and nuclear magnetic resonance spectroscopy to examine carbohydrate metabolism in vascular smooth muscle of freshly isolated pig cerebral microvessels (PCMV). PCMV utilized [2-(13)C]glucose mainly for glycolysis, producing [2-(13)C]lactate. Simultaneously, PCMV utilized the glycolytic intermediate [1-(13)C]fructose ...
Akbarali, H I Thatte, H He, X D Giles, W R Goyal, R K
Published in
The American journal of physiology
An inwardly rectifying K(+) conductance closely resembling the human ether-a-go-go-related gene (HERG) current was identified in single smooth muscle cells of opossum esophageal circular muscle. When cells were voltage clamped at 0 mV, in isotonic K(+) solution (140 mM), step hyperpolarizations to -120 mV in 10-mV increments resulted in large inwar...
Tyler, R C Fagan, K A Unfer, R C Gorman, C McClarrion, M Bullock, C Rodman, D M
Published in
The American journal of physiology
We report gene transfer to the normal and injured murine pulmonary circulation via systemic (intravascular) and airway (intratracheal) delivery of novel polycationic liposomes (imidazolium chloride, imidazolinium chloride-cholesterol, and ethyl phosphocholine). With use of the reporter genes chloramphenicol acetyltransferase (CAT) or human placenta...
Palmer, B M Olsson, M C Lynch, J M Mace, L C Snyder, S M Valent, S Moore, R L
Published in
The American journal of physiology
The effects of endurance run training on alpha-adrenergic responsiveness of rat left ventricle (LV) were examined in cardiomyocytes and isovolumic LV. Female Sprague-Dawley rats were sedentary (Sed) or trained (Tr) for >20 wk by treadmill running. Cardiomyocyte shortening and fura 2 fluorescence ratio were recorded before and during 5-min exposure ...
Ross, G F Ikegami, M Steinhilber, W Jobe, A H
Published in
The American journal of physiology
The developing lung contains surfactant protein (SP) C mRNA levels comparable to term values before mature type II cells and alveolar surfactant lipids are detectable. Estimates of the amount of mature SP-C in the alveolar lavages of preterm lungs are not available. We used an antibody to a recombinant human SP-C to measure the amount of SP-C in al...
Aeschbach, D Matthews, J R Postolache, T T Jackson, M A Giesen, H A Wehr, T A
Published in
The American journal of physiology
The influence of the circadian pacemaker and of the duration of time awake on the electroencephalogram (EEG) was investigated in 19 humans during approximately 40 h of sustained wakefulness. Two circadian rhythms in spectral power density were educed. The first rhythm was centered in the theta band (4.25-8.0 Hz) and exhibited a minimum approximatel...
Kawamura, H Otsuka, T Matsuno, H Niwa, M Matsui, N Kato, K Uematsu, T Kozawa, O
Published in
The American journal of physiology
We previously reported that endothelin-1 (ET-1) activates p42/p44 mitogen-activated protein (MAP) kinase in osteoblast-like MC3T3-E1 cells and consequently induces synthesis of interleukin-6. In the present study, we investigated the effect of ET-1 on the induction of heat shock protein 27 (HSP 27) in MC3T3-E1 cells. ET-1 time and dose dependently ...
Ku, N O Zhou, X Toivola, D M Omary, M B
Published in
The American journal of physiology
The mammalian cell cytoskeleton consists of a diverse group of fibrillar elements that play a pivotal role in mediating a number of digestive and nondigestive cell functions, including secretion, absorption, motility, mechanical integrity, and mitosis. The cytoskeleton of higher-eukaryotic cells consists of three highly abundant major protein famil...
