Barker, Roger A Carpenter, Melissa Jamieson, Catriona H M Murry, Charles E Pellegrini, Graziella Rao, Rajesh C Song, Jihwan
Published in
Stem cell reports
Developing cellular therapies is not straightforward. This Perspective summarizes the experience of a group of academic stem cell investigators working in different clinical areas and aims to share insight into what we wished we knew before starting. These include (1) choosing the stem cell line and assessing the genome of both the starting and fin...
Rudraraju, Rajeev Gartner, Matthew J Neil, Jessica A Stout, Elizabeth S Chen, Joseph Needham, Elise J See, Michael Mackenzie-Kludas, Charley Yang Lee, Leo Yi Wang, Mingyang
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Published in
Stem cell reports
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) primarily infects the respiratory tract, but pulmonary and cardiac complications occur in severe coronavirus disease 2019 (COVID-19). To elucidate molecular mechanisms in the lung and heart, we conducted paired experiments in human stem cell-derived lung alveolar type II (AT2) epithelial ...
Hysenaj, Lisiena Little, Samantha Kulhanek, Kayla Magnen, Melia Bahl, Kriti Gbenedio, Oghenekevwe M Prinz, Morgan Rodriguez, Lauren Andersen, Christopher Rao, Arjun Arkal
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Published in
Stem cell reports
Ancestral SARS coronavirus-2 (SARS-CoV-2) and variants of concern (VOC) caused a global pandemic with a spectrum of disease severity. The mechanistic explaining variations related to airway epithelium are relatively understudied. Here, we biobanked airway organoids (AO) by preserving stem cell function. We optimized viral infection with H1N1/PR8 an...
Sugarman, Jeremy Clark, Amander Fishkin, James Kato, Kazuto McCormack, Kevin Munsie, Megan Peluso, Michael J René, Nancy Solomon, Susan L
Published in
Stem cell reports
Public engagement is increasingly recognized as being integral to basic and translational research. Public engagement involves effective communication about research along with the mutual exchange of views and opinions among a wide variety of members in society. As such, public engagement can help to identify issues that must be addressed in order ...
Sureshchandra, Suhas Chan, Chi N Robino, Jacob J Parmelee, Lindsay K Nash, Michael J Wesolowski, Stephanie R Pietras, Eric M Friedman, Jacob E Takahashi, Diana Shen, Weining
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Published in
Stem cell reports
Maternal obesity adversely impacts the in utero metabolic environment, but its effect on fetal hematopoiesis remains incompletely understood. During late development, the fetal bone marrow (FBM) becomes the major site where macrophages and B lymphocytes are produced via differentiation of hematopoietic stem and progenitor cells (HSPCs). Here, we an...
Heinze, Dar Park, Seonmi McCracken, Andrew Haratianfar, Mona Lindstrom, Jonathan Villacorta-Martin, Carlos Mithal, Aditya Wang, Feiya Yang, Meng Wei Murphy, George
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Published in
Stem cell reports
A robust method of producing mature T cells from iPSCs is needed to realize their therapeutic potential. NOTCH1 is known to be required for the production of hematopoietic progenitor cells with T cell potential in vivo. Here we identify a critical window during mesodermal differentiation when Notch activation robustly improves access to definitive ...
Mayerl, Steven J Bajgai, Simona Ludwig, Allison L Jager, Lindsey D Williams, Brittany N Bacig, Cole Stoddard, Christopher Sinha, Divya Philpot, Benjamin D Gamm, David M
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Published in
Stem cell reports
Interphotoreceptor matrix proteoglycan 2 (IMPG2) mutations cause a severe form of early-onset retinitis pigmentosa (RP) with macular involvement. IMPG2 is expressed by photoreceptors and incorporated into the matrix that surrounds the inner and outer segments (OS) of rods and cones, but the mechanism of IMPG2-RP remains unclear. Loss of Impg2 funct...
Watanabe, Momoko Buth, Jessie E Haney, Jillian R Vishlaghi, Neda Turcios, Felix Elahi, Lubayna S Gu, Wen Pearson, Caroline A Kurdian, Arinnae Baliaouri, Natella V
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Published in
Stem cell reports
Telencephalic organoids generated from human pluripotent stem cells (hPSCs) are a promising system for studying the distinct features of the developing human brain and the underlying causes of many neurological disorders. While organoid technology is steadily advancing, many challenges remain, including potential batch-to-batch and cell-line-to-cel...
Pré, Deborah Wooten, Alexander T Biesmans, Steven Hinckley, Sandy Zhou, Haowen Sherman, Sean P Kakad, Priyanka Gearhart, Jeffrey Bang, Anne G
Published in
Stem cell reports
Impairment of long-term potentiation (LTP) is a common feature of many pre-clinical models of neurological disorders; however, studies in humans are limited by the inaccessibility of the brain. Human induced pluripotent stem cells (hiPSCs) provide a unique opportunity to study LTP in disease-specific genetic backgrounds. Here we describe a multi-el...
Garay, Bayardo I Givens, Sophie Abreu, Phablo Liu, Man Yücel, Doğacan Baik, June Stanis, Noah Rothermel, Taylor M Magli, Alessandro Abrahante, Juan E
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Published in
Stem cell reports
Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) provide great opportunities for mechanistic dissection of human cardiac pathophysiology; however, hiPSC-CMs remain immature relative to the adult heart. To identify novel signaling pathways driving the maturation process during heart development, we analyzed published transcript...