Schreiter, Sven Vafia, Katerina Barsacchi, Rico Tsang, Stephen H Bickle, Marc Ader, Marius Karl, Mike O Tanaka, Elly M Almedawar, Seba
Published in
Stem cell reports
Phagocytosis is a key function in various cells throughout the body. A deficiency in photoreceptor outer segment (POS) phagocytosis by the retinal pigment epithelium (RPE) causes vision loss in inherited retinal diseases and possibly age-related macular degeneration. To date, there are no effective therapies available aiming at recovering the lost ...
Pelham-Webb, Bobbie Murphy, Dylan Apostolou, Effie
Published in
Stem cell reports
Higher-order chromatin structure is tightly linked to gene expression and therefore cell identity. In recent years, the chromatin landscape of pluripotent stem cells has become better characterized, and unique features at various architectural levels have been revealed. However, the mechanisms that govern establishment and maintenance of these topo...
Lezmi, Elyad Weissbein, Uri Golan-Lev, Tamar Nissim-Rafinia, Malka Meshorer, Eran Benvenisty, Nissim
Published in
Stem cell reports
Chromatin regulators play fundamental roles in controlling pluripotency and differentiation. We examined the effect of mutations in 703 genes from nearly 70 chromatin-modifying complexes on human embryonic stem cell (ESC) growth. While the vast majority of chromatin-associated complexes are essential for ESC growth, the only complexes that conferre...
Seko, Daiki Fujita, Ryo Kitajima, Yuriko Nakamura, Kodai Imai, Yuuki Ono, Yusuke
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Stem cell reports
Estrogens are female sex hormones that are important for comprehensively maintaining muscle function, and an insufficiency affects muscle strength and regeneration in females. However, it is still unclear whether estrogen signaling is mediated through receptors. To investigate the specific role of estrogen receptor β (ERβ) in skeletal muscle and sa...
Lv, Peng Ma, Dongyuan Gao, Shuai Zhang, Yifan Bae, Young-Ki Liang, Guixian Gao, Suwei Choi, Jung-Hwa Kim, Cheol-Hee Wang, Lu
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Stem cell reports
Cell transplantation into immunodeficient recipients is a widely used approach to study stem cell and cancer biology; however, studying cell states post transplantation in vivo is inconvenient in mammals. Here, we generated a foxn1/Casper mutant zebrafish that is transparent and exhibits T cell deficiency. By employing the line for hematopoietic st...
Aulicino, Francesco Pedone, Elisa Sottile, Francesco Lluis, Frederic Marucci, Lucia Cosma, Maria Pia
Published in
Stem cell reports
The Wnt/β-catenin signaling pathway is a key regulator of embryonic stem cell (ESC) self-renewal and differentiation. Constitutive activation of this pathway has been shown to increase mouse ESC (mESC) self-renewal and pluripotency gene expression. In this study, we generated a novel β-catenin knockout model in mESCs to delete putatively functional...
Tao, Fang Soffers, Jelly Hu, Deqing Chen, Shiyuan Gao, Xin Zhang, Ying Zhao, Chongbei Smith, Sarah E Unruh, Jay R Zhang, Da
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Stem cell reports
Mouse embryonic stem cells (ESCs) cultured in defined medium resemble the pre-implantation epiblast in the ground state, with full developmental capacity including the germline. β-Catenin is required to maintain ground state pluripotency in mouse ESCs, but its exact role is controversial. Here, we reveal a Tcf3-independent role of β-catenin in rest...
Wang, Qingyu Lin, Yingying Sheng, Xiaole Xu, Jiuzhi Hou, Xuening Li, Yuan Zhang, Hao Guo, Huiyuan Yu, Zhengquan Ren, Fazheng
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Published in
Stem cell reports
Intestinal regeneration is crucial for functional restoration after injury, and nutritional molecules can play an important role in this process. Here, we found that arachidonic acid (AA) serves as a direct proliferation promoter of intestinal epithelial cells that facilitates small intestinal regeneration in both three-dimensional cultured organoi...
Sarikhani, Mohsen Garbern, Jessica C Ma, Sai Sereda, Rebecca Conde, Jeffrey Krähenbühl, Guido Escalante, Gabriela O Ahmed, Aishah Buenrostro, Jason D Lee, Richard T
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Stem cell reports
Recent studies suggest that metabolic regulation may improve differentiation of cardiomyocytes derived from induced pluripotent stem cells (iPSCs). AMP-activated protein kinase (AMPK) is a master regulator of metabolic activities. We investigated whether AMPK participates in iPSC-derived cardiomyocyte differentiation. We observed that AMPK phosphor...
Ahmed, Nouraiz Kunz, Leo Hoppe, Philipp S Loeffler, Dirk Etzrodt, Martin Ortega, Germán Camargo Hilsenbeck, Oliver Anastassiadis, Konstantinos Schroeder, Timm
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Stem cell reports
The transcription factor (TF) GATA2 plays a key role in organ development and cell fate control in the central nervous, urogenital, respiratory, and reproductive systems, and in primitive and definitive hematopoiesis. Here, we generate a knockin protein reporter mouse line expressing a GATA2VENUS fusion from the endogenous Gata2 genomic locus, with...
