Andor, Attila Mohanraj, Mahendravarman Pató, Zsuzsanna Anna Úri, Katalin Biri-Kovács, Beáta Cheng, Qing Arnér, Elias S J
Published in
Redox biology
TXNL1 (also named TRP32, for thioredoxin related protein of 32 kDa) is a cytosolic thioredoxin-fold protein expressed in all cell types and conserved from yeast to mammals, but with yet poorly known function. Here, we expressed and purified human TXNL1 together with several Cys-to-Ser variants, characterizing their enzymatic properties. TXNL1 could...
Peeters, W M Gram, M Dias, G J Vissers, M C M Hampton, M B Dickerhof, N Bekhit, A E Black, M J Oxbøll, J Bayer, S
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Published in
Redox biology
We recently developed a novel keratin-derived protein (KDP) rich in cysteine, glycine, and arginine, with the potential to alter tissue redox status and insulin sensitivity. The KDP was tested in 35 human adults with type-2 diabetes mellitus (T2DM) in a 14-wk randomised controlled pilot trial comprising three 2×20 g supplemental protein/day arms: K...
Kumar, Parveen Laurence, Emma Crossman, David K Assimos, Dean G Murphy, Michael P Mitchell, Tanecia
Published in
Redox biology
Oxalate is a small compound found in certain plant-derived foods and is a major component of calcium oxalate (CaOx) kidney stones. Individuals that consume oxalate enriched meals have an increased risk of forming urinary crystals, which are precursors to CaOx kidney stones. We previously reported that a single dietary oxalate load induces nanocryst...
Chu, Chengnan Wang, Xinyu Yang, Chao Chen, Fang Shi, Lin Xu, Weiqi Wang, Kai Liu, Baochen Wang, Chenyang Sun, Dongping
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Published in
Redox biology
Microvascular endothelial damage caused by intestinal ischemia‒reperfusion (II/R) is a primary catalyst for microcirculation dysfunction and enterogenous infection. Previous studies have mainly focused on how neutrophil extracellular traps (NETs) and ferroptosis cause intestinal epithelial injury, and little attention has been given to how NETs, ma...
Ferreira, Maria J Rodrigues, Tony A Pedrosa, Ana G Silva, Ana R Vilarinho, Beatriz G Francisco, Tânia Azevedo, Jorge E
Published in
Redox biology
Despite intensive research on peroxisome biochemistry, the role of glutathione in peroxisomal redox homeostasis has remained a matter of speculation for many years, and only recently has this issue started to be experimentally addressed. Here, we summarize and compare data from several organisms on the peroxisome-glutathione topic. It is clear from...
Zeng, Zihang Li, Tiao Liu, Xiangming Ma, Yiming Luo, Lijuan Wang, ZuLi Zhao, Zhiqi Li, Herui He, Xue Zeng, Huihui
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Published in
Redox biology
Chronic obstructive pulmonary disease (COPD) is a significant global cause of morbidity and mortality currently. Long-term exposure of cigarette smoke (CS) inducing persistent inflammation, small airway remodeling and emphysematous lung are the distinguishing features of COPD. Ferroptosis, occurred in lung epithelial cells has recently been reporte...
Roveri, Antonella Di Giacinto, Flavio Rossetto, Monica Cozza, Giorgio Cheng, Qing Miotto, Giovanni Zennaro, Lucio Di Paolo, Maria Luisa Arnér, Elias S J De Spirito, Marco
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Published in
Redox biology
The aim of this study was to examine, in biochemical detail, the functional role of the Arg152 residue in the selenoprotein Glutathione Peroxidase 4 (GPX4), whose mutation to His is involved in Sedaghatian-type Spondylometaphyseal Dysplasia (SSMD). Wild-type and mutated recombinant enzymes with selenopcysteine (Sec) at the active site, were purifie...
Khan, Muhammad Saad Shoaib Ahmed, Sulaiman Ikram, Aziz Ul Hannan, Fakhir Yasin, Muhammad Umair Wang, Jin Zhao, Biying Islam, Faisal Chen, Jian
Published in
Redox biology
Plants being sessile in nature, are exposed to unwarranted threats as a result of constantly changing environmental conditions. These adverse factors can have negative impacts on their growth, development, and yield. Hormones are key signaling molecules enabling cells to respond rapidly to different external and internal stimuli. In plants, melaton...
Xu, Shuqi Chuang, Christine Y Hawkins, Clare L Hägglund, Per Davies, Michael J
Published in
Redox biology
Peroxynitrous acid/peroxynitrite (ONOOH/ONOO-) is a powerful oxidizing/nitrating system formed at sites of inflammation, which can modify biological targets, and particularly proteins. Here, we show that multiple proteins from primary human coronary artery smooth muscle cells are nitrated, with LC-MS peptide mass mapping providing data on the sites...
Knoke, Lisa R Zimmermann, Jannik Lupilov, Natalie Schneider, Jannis F Celebi, Beyzanur Morgan, Bruce Leichert, Lars I
Published in
Redox biology
The thiol redox balance in the periplasm of E. coli depends on the DsbA/B pair for oxidative power and the DsbC/D system as its complement for isomerization of non-native disulfides. While the standard redox potentials of those systems are known, the in vivo "steady state" redox potential imposed onto protein thiol disulfide pairs in the periplasm ...
