Single-Molecule Studies of Actin Assembly and Disassembly Factors
Published in Methods in Enzymology
Single-Molecule Studies of Actin Assembly and Disassembly Factors
Cap-independent translation ensures mTOR expression and function upon protein synthesis inhibition
Published in RNA
The mechanistic/mammalian target of rapamycin (mTOR) is a conserved serine/threonine kinase that integrates cellular signals from the nutrient and energy status to act, namely, on the protein synthesis machinery. While major advances have emerged regarding the regulators and effects of the mTOR signaling pathway, little is known about the regulatio...
Cap-independent translation insures mTOR expression and function upon protein synthesis inhibition.
Published in RNA
The mechanistic/mammalian target of rapamycin (mTOR) is a conserved serine/threonine kinase that integrates cellular signals from the nutrient and energy status to act, namely, on the protein synthesis machinery. While major advances have emerged regarding the regulators and effects of the mTOR signaling pathway, little is known about the regulatio...
Brave new RNA world.
Published in RNA (New York, N.Y.)
Coffee with Ribohipster.
Published in RNA (New York, N.Y.)
Safer one-pot synthesis of the 'SHAPE' reagent 1-methyl-7-nitroisatoic anhydride (1m7).
Published in RNA (New York, N.Y.)
Estimating the reactivity of 2'-hydroxyl groups along an RNA chain of interest aids in the modeling of the folded RNA structure; flexible loops tend to be reactive, whereas duplex regions are generally not. Among the most useful reagents for probing 2'-hydroxyl reactivity is 1-methyl-7-nitroisatoic anhydride (1m7), but the absence of a reliable, in...
Quaking and PTB control overlapping splicing regulatory networks during muscle cell differentiation.
Published in RNA (New York, N.Y.)
Alternative splicing contributes to muscle development, but a complete set of muscle-splicing factors and their combinatorial interactions are unknown. Previous work identified ACUAA ("STAR" motif) as an enriched intron sequence near muscle-specific alternative exons such as Capzb exon 9. Mass spectrometry of myoblast proteins selected by the Capzb...
Mechanism of the AAA+ ATPases pontin and reptin in the biogenesis of H/ACA RNPs.
Published in RNA (New York, N.Y.)
The AAA+ ATPases pontin and reptin function in a staggering array of cellular processes including chromatin remodeling, transcriptional regulation, DNA damage repair, and assembly of macromolecular complexes, such as RNA polymerase II and small nucleolar (sno) RNPs. However, the molecular mechanism for all of these AAA+ ATPase associated activities...
Discovery of Pyrobaculum small RNA families with atypical pseudouridine guide RNA features.
Published in RNA (New York, N.Y.)
In the Eukarya and Archaea, small RNA-guided pseudouridine modification is believed to be an essential step in ribosomal RNA maturation. While readily modeled and identified by computational methods in eukaryotic species, these guide RNAs have not been found in most archaeal genomes. Using high-throughput transcriptome sequencing and comparative ge...
Production of artificial piRNAs in flies and mice.
Published in RNA (New York, N.Y.)
In animals a discrete class of small RNAs, the piwi-interacting RNAs (piRNAs), guard germ cell genomes against the activity of mobile genetic elements. piRNAs are generated, via an unknown mechanism, from apparently single-stranded precursors that arise from discrete genomic loci, termed piRNA clusters. Presently, little is known about the signals ...
