Smith, Benjamin A. Gelles, Jeff Goode, Bruce L.
Published in
Methods in Enzymology
The actin cytoskeleton is very dynamic and highly regulated by multiple associated proteins in vivo. Understanding how this system of proteins functions in the processes of actin network assembly and disassembly requires methods to dissect the mechanisms of activity of individual factors and of multiple factors acting in concert. The advent of sing...
Ana Ramos Coelho Candeias, Marco M. Menezes, Juliane Lacerda, Rafaela Willcocks, Margaret Teixeira, Alexandre Locker, Nicolas Romão, Luísa
Published in
RNA
The mechanistic/mammalian target of rapamycin (mTOR) is a conserved serine/threonine kinase that integrates cellular signals from the nutrient and energy status to act, namely, on the protein synthesis machinery. While major advances have emerged regarding the regulators and effects of the mTOR signaling pathway, little is known about the regulatio...
Ana Ramos Coelho
Published in
RNA
The mechanistic/mammalian target of rapamycin (mTOR) is a conserved serine/threonine kinase that integrates cellular signals from the nutrient and energy status to act, namely, on the protein synthesis machinery. While major advances have emerged regarding the regulators and effects of the mTOR signaling pathway, little is known about the regulatio...
Noller, Harry F
Published in
RNA (New York, N.Y.)
Ares, Manuel Jr
Published in
RNA (New York, N.Y.)
Turner, Rushia Shefer, Kinneret Ares, Manuel Jr
Published in
RNA (New York, N.Y.)
Estimating the reactivity of 2'-hydroxyl groups along an RNA chain of interest aids in the modeling of the folded RNA structure; flexible loops tend to be reactive, whereas duplex regions are generally not. Among the most useful reagents for probing 2'-hydroxyl reactivity is 1-methyl-7-nitroisatoic anhydride (1m7), but the absence of a reliable, in...
Hall, Megan P Nagel, Roland J Fagg, W Samuel Shiue, Lily Cline, Melissa S Perriman, Rhonda J Donohue, John Paul Ares, Manuel Jr
Published in
RNA (New York, N.Y.)
Alternative splicing contributes to muscle development, but a complete set of muscle-splicing factors and their combinatorial interactions are unknown. Previous work identified ACUAA ("STAR" motif) as an enriched intron sequence near muscle-specific alternative exons such as Capzb exon 9. Mass spectrometry of myoblast proteins selected by the Capzb...
Machado-Pinilla, Rosario Liger, Dominique Leulliot, Nicolas Meier, U Thomas
Published in
RNA (New York, N.Y.)
The AAA+ ATPases pontin and reptin function in a staggering array of cellular processes including chromatin remodeling, transcriptional regulation, DNA damage repair, and assembly of macromolecular complexes, such as RNA polymerase II and small nucleolar (sno) RNPs. However, the molecular mechanism for all of these AAA+ ATPase associated activities...
Muerdter, Felix Olovnikov, Ivan Molaro, Antoine Rozhkov, Nikolay V Czech, Benjamin Gordon, Assaf Hannon, Gregory J Aravin, Alexei A
Published in
RNA (New York, N.Y.)
In animals a discrete class of small RNAs, the piwi-interacting RNAs (piRNAs), guard germ cell genomes against the activity of mobile genetic elements. piRNAs are generated, via an unknown mechanism, from apparently single-stranded precursors that arise from discrete genomic loci, termed piRNA clusters. Presently, little is known about the signals ...
Bateman, Alex Agrawal, Shipra Birney, Ewan Bruford, Elspeth A Bujnicki, Janusz M Cochrane, Guy Cole, James R Dinger, Marcel E Enright, Anton J Gardner, Paul P
...
Published in
RNA (New York, N.Y.)
During the last decade there has been a great increase in the number of noncoding RNA genes identified, including new classes such as microRNAs and piRNAs. There is also a large growth in the amount of experimental characterization of these RNA components. Despite this growth in information, it is still difficult for researchers to access RNA data,...