Melik-Nubarov, N S Grozdova, I D Lomakina, G Yu Pokrovskaya, M V Pokrovski, V S Aleksandrova, S S Abakumova, O Yu Podobed, O V Grishin, D V Sokolov, N N
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Prikladnaia biokhimiia i mikrobiologiia
N-hydroxysuccinimide ester of monomethoxy polyethylene glycol hemisuccinate was synthesized. It acylated amino groups in a molecule of recombinant L-asparaginase from Erwinia carotovora. A method of L-asparaginase modification by the obtained activated polyethylene glycol derivative was developed. The best results were produced by modification of t...
Petukhova, N I Kon'shina, I I Spivak, A Yu Odinokov, V N Zorin, V V
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Prikladnaia biokhimiia i mikrobiologiia
A novel promising strain of actinobacteria Rhodococcus sp. 77-32 was identified. Its acetonetreated biomass the could be used as a biocatalyst for production of S-(-)-2-[6-benzyloxy-2,5,7,8-tetramethylchroman-2-yl] ethanol (S-BCE), a precursor of natural α-tocols. It was established that a reaction of enantioselective hydrolysis of racemic (±)-2-(2...
Barsukova, M E Tokareva, A I Buslova, T S Malinina, L I Veselova, I A Shekhovtsova, T N
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Prikladnaia biokhimiia i mikrobiologiia
The kinetics of oxidation reactions of flavonoids, quercetin, dihydroquercetin, and epicatechin has been studied in the presence of biocatalysts of different natures: horseradish peroxidase, mushroom tyrosinase, and hemoglobin from bull blood. Comparison of the kinetic parameters of the oxidation reaction showed that peroxidase appeared to be the m...
Onishchuk, O P Vorobyov, N I Provorov, N A
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Prikladnaia biokhimiia i mikrobiologiia
The most recent data on the system of cmp (competitiveness) genes that determine the nodulation competitiveness of rhizobial strains, i.e., the ability to compete for nodule formation in leguminous plants, is analyzed. Three genetic approaches for the construction of economically valuable strains of rhizobia are proposed: the amplification of posit...
Yantsevich, A V Dzichenka, Ya V Ivanchik, A V Shapiro, M A Trawkina, M Shkel, T V Gilep, A A Sergeev, G V Usanov, S A
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Prikladnaia biokhimiia i mikrobiologiia
Contaminating proteins have been identified by “shotgun” proteomic analysis in 14 recombinant preparations of human membrane heme- and flavoproteins expressed in Escherichia coli and purified by immobilized metal ion affinity chromatography. Immobilized metal ion affinity chromatography of ten proteins was performed on Ni2+-NTA-sepharose 6B, and th...
Glyan'ko, A K Ischenko, A A
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Prikladnaia biokhimiia i mikrobiologiia
Recent studies of the immune system of leguminous plants infected with nodular bacteria (rhizobia) are summarized. The possibility of blocking the invasion of rhizobia into plant organs not affected by the primary infection is discussed. The concept of local and systemic resistance of the leguminous plant to rhizobial infection is introduced. The N...
Firsova, Yu E Torgonskaya, M L Trotsenko, Yu A
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Prikladnaia biokhimiia i mikrobiologiia
A knockout mutant of Methylobacterium dichloromethanicum DM4 with an inactivated gene of a putative transcription regulator METDI5511 (ΔMETDI5511) has been obtained. The expression of this gene increases many times when the strain is grown on dichloromethane compared to methanol. The mutant had a low growth rate on dichloromethane as compared with ...
Tikhonova, T V Slutskaya, E S Popov, V O
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Prikladnaia biokhimiia i mikrobiologiia
Closely related penta- and octaheme nitrite reductases catalyze the reduction of nitrite, nitric oxide, and hydroxylamine to ammonium and of sulfite to sulfide. NrfA pentaheme nitrite reductase plays the key role in anaerobic nitrate respiration and the protection of bacterial cells from stresses caused by nitrogen oxides and hydrogen peroxide. Oct...
Zakharchenko, N S Rukavtsova, E B Shevchuk, T V Furs, O V Pigoleva, S V Lebedeva, A A Chulina, I A Baidakova, L K Bur'yanov, Ya I
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Prikladnaia biokhimiia i mikrobiologiia
Kalanchoe pinnata L. plants bearing an artificial CP1 gene encoding the cecropin P1 antimicrobial peptide have been obtained. The presence of the CP1 gene in the plant genome has been confirmed by PCR. Cecropin P1 synthesis in transgenic plants has been shown by MALDI mass spectrometry and Western blotting. The obtained plants have been highly resi...
Gulii, O I Karavayeva, O A Pavlii, S A Sokolov, O I Bunin, V D Ignatov, O V
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Prikladnaia biokhimiia i mikrobiologiia
The dependence-of changes in the electrooptical properties of Azospirillum brasilense cell suspension Sp7 during interaction with bacteriophage ΦAb-Sp7 on the number and time of interactions was studied. Incubation of cells with bacteriophage significantly changed the electrooptical signal within one minute. The selective effect of bacteriophage ΦA...
