Boontun, Chayanee Vatanyoopaisarn, Savitri Hankla, Sungwarn Kuraya, Eisuke Tamaki, Yasutomo
Published in
Preparative biochemistry & biotechnology
Probiotic bacteria continue to receive increasing attention in the food and feed industries. However, the production of Bifidobacterium and Lactobacillus at an industrial scale is challenging because of specific nutrient requirements and conditions, which are complicated and costly. We developed low-cost culture media by modifying the carbon and ni...
Tayebati, Hanieh Pajoum Shariati, Farshid Soltani, Neda Sepasi Tehrani, Hessam
Published in
Preparative biochemistry & biotechnology
Today, the use of nutrients derived from natural bioactive compounds application in the food, pharmaceutical, and cosmetic industries is on the increase. This paper aimed to evaluate the amino acids profile (essential and non-essential) and pigments composition (chlorophyll a, carotenoids, and phycocyanin) of Arthrospira platensis (a blue-green mic...
Rmili, Fatma Krayem, Najeh Loiseau, Celine Gauvry, Laurent Frikha, Fakher Ergan, Françoise Chamkha, Mohamed Sayari, Adel Fendri, Ahmed
Published in
Preparative biochemistry & biotechnology
The study illustrated here aims on an organic solvent tolerant lipase from Staphylococcus capitis (SCL). The gene part, encoding the mature lipase, was cloned and sequenced. The concluded polypeptide sequence, equivalent to the protein, consist of 388 amino acid residues with a molecular mass of about 45 kDa. A structure-based alignment of the SCL ...
Letchumanan, Kahviyaah Abdullah, Nor Hayati Abdul-Aziz, Azila
Published in
Preparative biochemistry & biotechnology
Dynamic maceration facilitates diffusion in solid-liquid extraction through controlling temperature and providing agitation. However, equipment design for dynamic maceration in previous investigations resulted in inadequate homogeneity of temperature and solid dispersion. A laboratory scale extractor was designed to aid the heat and mass transfer p...
Nasser, Tarlan Emamshoushtari, Mir Mehrshad Helchi, Salar Saeidi, Ardeshir Pajoum Shariati, Farshid
Published in
Preparative biochemistry & biotechnology
Membrane photobioreactors (MPBRs) have gained significant attention due to their ability to support microalgae activities such as cultivation, harvesting, and production of beneficial products. Despite various efforts to mitigate membrane fouling, a fundamental issue in membrane processes, in these systems, a cost-effective and less energy-consumin...
Kidd, E Meimaridou, E Williams, J Metherell, L A Walley, A J Fairbrother, U L
Published in
Preparative biochemistry & biotechnology
Telomere Length (TL) and integrity is significantly associated with age-related disease, multiple genetic and environmental factors. We observe mouse genomic DNA (gDNA) isolation methods to have a significant impact on average TL estimates. The canonical qPCR method does not measure TL directly but via the ratio of telomere repeats to a single copy...
Vieira, Matheus Maitan Valadares, Fernanda Lima Velasco, Josman da Silva, Silvio S Segato, Fernando Chandel, Anuj K
Published in
Preparative biochemistry & biotechnology
Aureobasidium pullulans LB83 is a versatile biocatalyst that produces a plethora of bioactive products thriving on a variety of feedstocks under the varying culture conditions. In our last study using this microorganism, we found cellulase activity (FPase, 2.27 U/ml; CMCase, 7.42 U/ml) and other plant cell wall degrading enzyme activities grown on ...
Verma, Himanshu Mihooliya, Kanti N Nandal, Jitender Sahoo, Debendra K
Published in
Preparative biochemistry & biotechnology
The marine environment is known for its vast diversity of the microbial population; however, less explored for bioactive compounds. In this study, an AMP produced by a new marine isolate, Vibrio proteolyticus MT110, showed broad-spectrum antimicrobial activity against Gram-positive and Gram-negative bacteria. The AMP was purified to homogeneity usi...
Che Hussian, Che Haznie Ayu Leong, Wai Yie
Published in
Preparative biochemistry & biotechnology
Therapeutic proteins are recombinant proteins generated through recombinant DNA technology and have attracted a great deal of interest in numerous applications, including pharmaceutical, cosmetic, human and animal health, agriculture, food, and bioremediation. Producing therapeutic proteins on a large scale, mainly in the pharmaceutical industry, n...
Costa, Elizianne Pereira Brandão-Costa, Romero Marcos Pedrosa Albuquerque, Wendell Wagner Campos Nascimento, Thiago Pajeú Sales Conniff, Amanda Emmanuelle Cardoso, Kethylen Barbara Barbosa Neves, Anna Gabrielly Duarte Batista, Juanize Matias da Silva Porto, Ana Lúcia Figueiredo
Published in
Preparative biochemistry & biotechnology
Collagenases are proteases able to degrade native and denatured collagen, with broad applications such as leather, food, and pharmaceutical industries. The aim of this research was to purify and characterize a collagenase from Streptomyces antibioticus. In the present work, the coffee ground substrate provided conditions to obtaining high collagena...
