Maltas, Jeff Wood, Kevin B
Published in
PLoS biology
Evolved resistance to one antibiotic may be associated with "collateral" sensitivity to other drugs. Here, we provide an extensive quantitative characterization of collateral effects in Enterococcus faecalis, a gram-positive opportunistic pathogen. By combining parallel experimental evolution with high-throughput dose-response measurements, we meas...
Planade, Jessica Belbahri, Reda Boiero Sanders, Micaela Guillotin, Audrey du Roure, Olivia Michelot, Alphée Heuvingh, Julien
Published in
PLoS biology
Clathrin-mediated endocytosis involves the sequential assembly of more than 60 proteins at the plasma membrane. An important fraction of these proteins regulates the assembly of an actin-related protein 2/3 (Arp2/3)-branched actin network, which is essential to generate the force during membrane invagination. We performed, on wild-type (WT) yeast a...
Henderson, Nicholas T Pablo, Michael Ghose, Debraj Clark-Cotton, Manuella R Zyla, Trevin R Nolen, James Elston, Timothy C Lew, Daniel J
Published in
PLoS biology
Accurate detection of extracellular chemical gradients is essential for many cellular behaviors. Gradient sensing is challenging for small cells, which can experience little difference in ligand concentrations on the up-gradient and down-gradient sides of the cell. Nevertheless, the tiny cells of the yeast Saccharomyces cerevisiae reliably decode g...
Puñal, Vanessa M Paisley, Caitlin E Brecha, Federica S Lee, Monica A Perelli, Robin M Wang, Jingjing O'Koren, Emily G Ackley, Caroline R Saban, Daniel R Reese, Benjamin E
...
Published in
PLoS biology
Naturally occurring cell death is a fundamental developmental mechanism for regulating cell numbers and sculpting developing organs. This is particularly true in the nervous system, where large numbers of neurons and oligodendrocytes are eliminated via apoptosis during normal development. Given the profound impact of death upon these two major cell...
Zhou, Yandong Nwokonko, Robert M Baraniak, James H Jr Trebak, Mohamed Lee, Kenneth P K Gill, Donald L
Published in
PLoS biology
Calcium signals drive an endless array of cellular responses including secretion, contraction, transcription, cell division, and growth. The ubiquitously expressed Orai family of plasma membrane (PM) ion channels mediate Ca2+ entry signals triggered by the Ca2+ sensor Stromal Interaction Molecule (STIM) proteins of the endoplasmic reticulum (ER). T...
Kumaran, Dharshan Maguire, Eleanor A
Published in
PLoS biology
[This corrects the article DOI: 10.1371/journal.pbio.0040424.].
Beeby, Morgan
Published in
PLoS biology
Case studies of the evolution of molecular machines remain scarce. One of the most diverse and widespread homologous families of machines is the type IV filament (TFF) superfamily, comprised of type IV pili, type II secretion systems (T2SSs), archaella, and other less-well-characterized families. These families have functions including twitching mo...
Umehara, Takashi Tsujita, Natsumi Shimada, Masayuki
Published in
PLoS biology
In most mammals, the male to female sex ratio of offspring is about 50% because half of the sperm contain either the Y chromosome or X chromosome. In mice, the Y chromosome encodes fewer than 700 genes, whereas the X chromosome encodes over 3,000 genes. Although overall gene expression is lower in sperm than in somatic cells, transcription is activ...
Lee, Dongyeop An, Seon Woo A Jung, Yoonji Yamaoka, Yasuyo Ryu, Youngjae Goh, Grace Ying Shyen Beigi, Arshia Yang, Jae-Seong Jung, Gyoo Yeol Ma, Dengke K
...
Published in
PLoS biology
Low temperatures delay aging and promote longevity in many organisms. However, the metabolic and homeostatic aspects of low-temperature-induced longevity remain poorly understood. Here, we show that lipid homeostasis regulated by Caenorhabditis elegans Mediator 15 (MDT-15 or MED15), a transcriptional coregulator, is essential for low-temperature-in...
Jia, Ru Li, Dongdong Li, Ming Chai, Yongping Liu, Yufan Xie, Zhongyun Shao, Wenxin Xie, Chao Li, Liuju Huang, Xiaoshuai
...
Published in
PLoS biology
Cilia are remarkable cellular devices that power cell motility and transduce extracellular signals. To assemble a cilium, a cylindrical array of 9 doublet microtubules push out an extension of the plasma membrane. Membrane tension regulates cilium formation; however, molecular pathways that link mechanical stimuli to ciliogenesis are unclear. Using...