New rules for genomics-informed COVID-19 responses-Lessons learned from the first waves of the Omicron variant in Austra...
Published in PLoS genetics
New rules for genomics-informed COVID-19 responses-Lessons learned from the first waves of the Omicron variant in Austra...
The entry of unclosed autophagosomes into vacuoles and its physiological relevance.
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Published in PLoS genetics
It is widely stated in the literature that closed mature autophagosomes (APs) fuse with lysosomes/vacuoles during macroautophagy/autophagy. Previously, we showed that unclosed APs accumulated as clusters outside vacuoles in Vps21/Rab5 and ESCRT mutants after a short period of nitrogen starvation. However, the fate of such unclosed APs remains uncle...
Phosphorylation of MSI-1 is implicated in the regulation of associative memory in Caenorhabditis elegans.
Published in PLoS genetics
The Musashi family of RNA-binding proteins controls several biological processes including stem cell maintenance, cell division and neural function. Previously, we demonstrated that the C. elegans Musashi ortholog, msi-1, regulates forgetting via translational repression of the Arp2/3 actin-branching complex. However, the mechanisms controlling MSI...
A two–tiered system for selective receptor and transporter protein degradation
Published in PLoS Genetics
Receptor, transporter and channel proteins on the plasma membranes (or surface) of all cells mediate extensive physiology. This requires precise control of their numbers, and damaged copies must be removed to prevent cytotoxicity. Their downregulation and degradation is mediated by lysosomes after endocytosis and entry into the multi–vesicular body...
Antisense non-coding transcription represses the PHO5 model gene at the level of promoter chromatin structure
Published in PLoS Genetics
Non-coding transcripts synthesized by RNA Poll are short-lived because they are rapidly terminated by the NNS complex and degraded by the RNA exosome. However, some non-coding transcripts can regulate the expression of coding genes at whose loci they are initiated. In this work, we show that a non-coding antisense transcript regulates the expressio...
Nuclear position modulates long-range chromatin interactions.
Published in PLoS genetics
The human genome is non-randomly organized within the cell nucleus. Spatial mapping of genome folding by biochemical methods and imaging has revealed extensive variation in locus interaction frequencies between cells in a population and between homologs within an individual cell. Commonly used mapping approaches typically examine either the relativ...
Functional analysis of a bitter gustatory receptor highly expressed in the larval maxillary galea of Helicoverpa armiger...
Published in PLoS Genetics
Phytophagous insects avoid eating most plants because of the presence of distasteful secondary compounds, which introduces the question of how these compounds are perceived by insects. The cotton bollworm is a very serious crop pest in the Old World, and its caterpillars mainly feed on the reproductive organs of many host plants. Here, we study the...
The RNA helicase DDX6 controls early mouse embryogenesis by repressing aberrant inhibition of BMP signaling through miRN...
Published in PLoS genetics
The evolutionarily conserved RNA helicase DDX6 is a central player in post-transcriptional regulation, but its role during embryogenesis remains elusive. We here show that DDX6 enables proper cell lineage specification from pluripotent cells by analyzing Ddx6 knockout (KO) mouse embryos and employing an in vitro epiblast-like cell (EpiLC) induction...
miR-275/305 cluster is essential for maintaining energy metabolic homeostasis by the insulin signaling pathway in Bactro...
Published in PLoS genetics
Increasing evidence indicates that miRNAs play crucial regulatory roles in various physiological processes of insects, including systemic metabolism. However, the molecular mechanisms of how specific miRNAs regulate energy metabolic homeostasis remain largely unknown. In the present study, we found that an evolutionarily conserved miR-275/305 clust...
Phosphorylated histone variant γH2Av is associated with chromatin insulators in Drosophila.
Published in PLoS genetics
Chromatin insulators are responsible for orchestrating long-range interactions between enhancers and promoters throughout the genome and align with the boundaries of Topologically Associating Domains (TADs). Here, we demonstrate an association between gypsy insulator proteins and the phosphorylated histone variant H2Av (γH2Av), normally a marker of...
