Rakshit, Tatini Melters, Daniël P Dimitriadis, Emilios K Dalal, Yamini
Published in
Nucleus (Austin, Tex.)
The interplay between transcription factors, chromatin remodelers, 3-D organization, and mechanical properties of the chromatin fiber controls genome function in eukaryotes. Besides the canonical histones which fold the bulk of the chromatin into nucleosomes, histone variants create distinctive chromatin domains that are thought to regulate transcr...
Koch, Bailey A Staley, Elizabeth Jin, Hui Yu, Hong-Guo
Published in
Nucleus (Austin, Tex.)
Cellular aging occurs as a cell loses its ability to maintain homeostasis. Aging cells eliminate damaged cellular compartments and other senescence factors via self-renewal. The mechanism that regulates cellular rejuvenation remains to be further elucidated. Using budding yeast gametogenesis as a model, we show here that the endosomal sorting compl...
Burla, Romina La Torre, Mattia Maccaroni, Klizia Verni, Fiammetta Giunta, Simona Saggio, Isabella
Published in
Nucleus (Austin, Tex.)
The nuclear envelope compartmentalizes chromatin in eukaryotic cells. The main nuclear envelope components are lamins that associate with a panoply of factors, including the LEM domain proteins. The nuclear envelope of mammalian cells opens up during cell division. It is reassembled and associated with chromatin at the end of mitosis when telomeres...
Murawska, Magdalena Ladurner, Andreas G
Published in
Nucleus (Austin, Tex.)
Bromodomain AAA+ ATPases (ATPases associated with diverse cellular activities) are emerging as oncogenic proteins and compelling targets for anticancer therapies. However, structural and biochemical insight into these machines is missing. A recent study by Cho et al. reports the first cryo-EM structure of a bromodomain AAA+ ATPase and provides firs...
Takahashi, Yuto Hiratsuka, Shogo Machida, Nanako Takahashi, Daisuke Matsushita, Junpei Hozak, Pavel Misteli, Tom Miyamoto, Kei Harata, Masahiko
Published in
Nucleus (Austin, Tex.)
Hutchinson-Gilford progeria syndrome (HGPS) is a premature aging disorder caused by a mutation of lamin A, which contributes to nuclear architecture and the spatial organization of chromatin in the nucleus. The expression of a lamin A mutant, named progerin, leads to functional and structural disruption of nuclear organization. Since progerin lacks...
Gumber, Hardeep K McKenna, Joseph F Tolmie, Andrea F Jalovec, Alexis M Kartick, Andre C Graumann, Katja Bass, Hank W
Published in
Nucleus (Austin, Tex.)
The linker of nucleoskeleton and cytoskeleton (LINC) complex is an essential multi-protein structure spanning the eukaryotic nuclear envelope. The LINC complex functions to maintain nuclear architecture, positioning, and mobility, along with specialized functions in meiotic prophase and chromosome segregation. Members of the LINC complex were recen...
Kim, Youngjo Zheng, Xiaobin Zheng, Yixian
Published in
Nucleus (Austin, Tex.)
Genome-wide mapping of lamin-B1-genome interactions has shown that gene-poor and transcriptionally inactive genomic regions are associated with the nuclear lamina. Numerous studies have suggested that lamins, the major structural components of the nuclear lamina, play a role in global chromatin organization and gene expression. How lamins could inf...
Milbury, Karissa L Paul, Biplab Lari, Azra Fowler, Claire Montpetit, Ben Stirling, Peter C
Published in
Nucleus (Austin, Tex.)
The exosome functions to regulate the cellular transcriptome through RNA biogenesis, surveillance, and decay. Mutations in Dis3, a catalytic subunit of the RNA exosome with separable endonuclease and exonuclease activities, are linked to multiple myeloma. Here we report that a cancer-associated DIS3 allele, dis3E729K, provides evidence for DIS3 fun...
Paouneskou, Dimitra Jantsch, Verena
Published in
Nucleus (Austin, Tex.)
Active meiotic chromosome movements are a universally conserved feature. They occur at the early stages of prophase of the first meiotic division and support the chromosome pairing process by (1) efficiently installing the synaptonemal complex between homologous chromosomes, (2) discouraging inadvertent chromosome interactions and (3) bringing homo...
Dreger, Marcel Madrazo, Elena Hurlstone, Adam Redondo-Muñoz, Javier
Published in
Nucleus (Austin, Tex.)
Migrating cells have to cross many physical barriers and confined in 3D environments. The surrounding environment promotes mechano- and biological signals that orchestrate cellular changes, such as cytoskeletal and adhesion rearrangements and proteolytic digestion. Recent studies provide new insights into how the nucleus must alter its shape, local...