Kamp, Juliette A Lemmens, Bennie B L G Romeijn, Ron J González-Prieto, Román Olsen, Jesper V Vertegaal, Alfred C O van Schendel, Robin Tijsterman, Marcel
Published in
Nucleic acids research
The integrity and proper expression of genomes are safeguarded by DNA and RNA surveillance pathways. While many RNA surveillance factors have additional functions in the nucleus, little is known about the incidence and physiological impact of converging RNA and DNA signals. Here, using genetic screens and genome-wide analyses, we identified unfores...
Vasileva, Delyana P Streich, Jared C Burdick, Leah H Klingeman, Dawn M Chhetri, Hari B Brelsford, Christa M Ellis, J Christopher Close, Dan M Jacobson, Daniel A Michener, Joshua K
...
Published in
Nucleic acids research
In eukaryotes, fine-scale maps of meiotic recombination events have greatly advanced our understanding of the factors that affect genomic variation patterns and evolution of traits. However, in bacteria that lack natural systems for sexual reproduction, unbiased characterization of recombination landscapes has remained challenging due to variable r...
Ai, Yuxi Liang, Dongming Wilusz, Jeremy E
Published in
Nucleic acids research
CRISPR/Cas13 effectors have garnered increasing attention as easily customizable tools for detecting and depleting RNAs of interest. Near perfect complementarity between a target RNA and the Cas13-associated guide RNA is required for activation of Cas13 ribonuclease activity. Nonetheless, the specificity of Cas13 effectors in eukaryotic cells has b...
Gorbea, Carlos Elhakiem, Abdalla Cazalla, Demián
Published in
Nucleic acids research
HSUR1 and HSUR2, two noncoding RNAs expressed by the oncogenic Herpesvirus saimiri, bind host microRNAs miR-142-3p, miR-16, and miR-27 with different purposes. While binding of miR-27 to HSUR1 triggers the degradation of the microRNA, miR-16 is tethered by HSUR2 to target host mRNAs to repress their expression. Here we show that the interaction wit...
Yang, Mingming Wang, Chenliang Zhou, Mi Bao, Lei Wang, Yanan Kumar, Ashwani Xing, Chao Luo, Weibo Wang, Yingfei
Published in
Nucleic acids research
Poly(ADP-ribose) polymerase-1 (PARP-1) is a DNA damage sensor and contributes to both DNA repair and cell death processes. However, how PARP-1 signaling is regulated to switch its function from DNA repair to cell death remains largely unknown. Here, we found that PARP-1 plays a central role in alkylating agent-induced PARthanatic cancer cell death....
Tomkuvienė, Miglė Meier, Markus Ikasalaitė, Diana Wildenauer, Julia Kairys, Visvaldas Klimašauskas, Saulius Manelytė, Laura
Published in
Nucleic acids research
Methylation of cytosine to 5-methylcytosine (mC) at CpG sites is a prevalent reversible epigenetic mark in vertebrates established by DNA methyltransferases (MTases); the attached methyl groups can alter local structure of DNA and chromatin as well as binding of dedicated proteins. Nucleosome assembly on methylated DNA has been studied extensively,...
Riehl, Kevin Riccio, Cristian Miska, Eric A Hemberg, Martin
Published in
Nucleic acids research
Most genomes harbor a large number of transposons, and they play an important role in evolution and gene regulation. They are also of interest to clinicians as they are involved in several diseases, including cancer and neurodegeneration. Although several methods for transposon identification are available, they are often highly specialised towards...
Miele, Solange Provan, James Iain Vergne, Justine Possoz, Christophe Ochsenbein, Françoise Barre, François-Xavier
Published in
Nucleic Acids Research
The chromosome dimer resolution machinery of bacteria is generally composed of two tyrosine recombinases, XerC and XerD. They resolve chromosome dimers by adding a crossover between sister copies of a specific site, dif . The reaction depends on a cell division protein, FtsK, which activates XerD by protein-protein interactions. The toxin-linked cr...
Bortolin-Cavaillé, Marie-Line Quillien, Aurélie Thalalla Gamage, Supuni Thomas, Justin M Sas-Chen, Aldema Sharma, Sunny Plisson-Chastang, Célia Vandel, Laurence Blader, Patrick Lafontaine, Denis L J
...
Published in
Nucleic acids research
NAT10 is an essential enzyme that catalyzes N4-acetylcytidine (ac4C) in eukaryotic transfer RNA and 18S ribosomal RNA. Recent studies suggested that rRNA acetylation is dependent on SNORD13, a box C/D small nucleolar RNA predicted to base-pair with 18S rRNA via two antisense elements. However, the selectivity of SNORD13-dependent cytidine acetylati...
Mestre, Mario Rodríguez Gao, Linyi Alex Shah, Shiraz A López-Beltrán, Adrián González-Delgado, Alejandro Martínez-Abarca, Francisco Iranzo, Jaime Redrejo-Rodríguez, Modesto Zhang, Feng Toro, Nicolás
...
Published in
Nucleic acids research
Reverse transcriptases (RTs) are enzymes capable of synthesizing DNA using RNA as a template. Within the last few years, a burst of research has led to the discovery of novel prokaryotic RTs with diverse antiviral properties, such as DRTs (Defense-associated RTs), which belong to the so-called group of unknown RTs (UG) and are closely related to th...
