Published in Nature Reviews Methods Primers
This Primer provides an overview of the general principles of adaptive optics and explores the different ways in which adaptive optics can correct optical aberrations for high-resolution imaging in the fields of astronomy, vision science and microscopy.
Published in Nature Reviews Methods Primers
Tissue clearing techniques render biological samples transparent to allow for the deep imaging of large tissue volumes. This Primer presents tissue clearing techniques in a modular way and underlines the main principles of each stage of clearing to help researchers to develop a custom tissue clearing workflow for their sample of choice. The authors...
Published in Frontiers in Systems Neuroscience
The honeybee standard brain (HSB) serves as an interactive tool for relating morphologies of bee brain neurons and provides a reference system for functional and bibliographical properties (http://www.neurobiologie.fu-berlin.de/beebrain/). The ultimate goal is to document not only the morphological network properties of neurons collected from separ...
Published in Frontiers in Applied Mathematics and Statistics
Many brain-based disorders are traditionally diagnosed based on clinical interviews and behavioral assessments, which are recognized to be largely imperfect. Therefore, it is necessary to establish neuroimaging-based biomarkers to improve diagnostic precision. Resting-state functional magnetic resonance imaging (rs-fMRI) is a promising technique fo...
Published in Frontiers in Systems Neuroscience
Functional MRI measured with blood oxygen dependent (BOLD) contrast in the absence of intermittent tasks reflects spontaneous activity of so-called resting state networks (RSN) of the brain. Group level independent component analysis (ICA) of BOLD data can separate the human brain cortex into 42 independent RSNs. In this study we evaluated age-rela...
Published in Nature Reviews Methods Primers
3D extrusion bioprinting methods can be used to produce tissue constructs in vitro and in situ and are arguably the most commonly used bioprinting strategies. In this Primer, Zhang and colleagues describe the variants of 3D extrusion bioprinting methods and their specific applications, considerations for the formulation of bioinks and strategies fo...
Published in Nature Reviews Methods Primers
Muon spin spectroscopy examines how muons interact with their local environment through measurement of the muon’s precession and relaxation. This provides unique information about the static and dynamic properties of a material. This Primer gives an introductory overview to muon spin spectroscopy, describing how muons are produced and used experime...
Published in Nature Reviews Methods Primers
DNA-encoded chemical libraries (DECLs) are used in the discovery of small molecules with potential therapeutic benefit. In this Primer, Satz et al. describe the best practices for DECL synthesis, selection and analysis, as well as requirements to enhance reproducibility between and within groups.
Published in Nature Reviews Methods Primers
Surface-enhanced Raman spectroscopy (SERS) uses nanostructured materials to enhance the Raman scattering of a sample, enhancing sensitivity and allowing analysis of materials in low concentration. In this Primer, Han et al. detail the use of SERS equipment and preparation of SERS-active materials, as well as recent applications in biological and ch...
Published in Nature Reviews Methods Primers
Whispering-gallery-mode sensors use multiple-cavity passes of light for very sensitive measurements at the microscale and nanoscale. This Primer introduces various whispering-gallery-mode sensor setups, describes different sensing mechanisms and highlights in vivo and single-molecule sensing applications in biology and physics.
