Published in Nature Methods
A multi-laboratory study in the form of a community challenge assesses the quality of models that can be produced from cryo-EM maps using different software tools, the reproducibility of models generated by different users and the performance of metrics used for model validation.
Published in Nature Methods
A two-photon miniature microscope with enlarged field of view and axial scanning capabilities has been developed and applied in freely moving mice.
Published in Nature Methods
Tessa, a Bayesian model, integrates TCR sequencing data with gene expression data to analyze the effect of the TCR repertoire on T cell functionality.
Published in Nature Methods
“We demand rigidly defined areas of doubt and uncertainty!” —D. Adams
Published in Nature Methods
Researchers are putting new spins on familiar dyes and showing their versatility for labeling living systems.
Published in Nature Methods
As single-cell omics continue to advance, the field of spatially resolved transcriptomics has emerged with a set of experimental and computational methods to map out the positions of cells and their gene expression profiles in space. Here we summarize current transcriptome-wide and sequencing-based methodologies and their applications in genomics r...
Published in Nature Methods
Microfluidic channels provide a means to deliver barcodes encoding spatial information to a tissue, which allows co-profiling of gene expression and proteins of interest in a spatially resolved manner.
Published in Nature Methods
Megabodies, built by grafting nanobodies onto larger protein scaffolds, help alleviate problems of particle size and preferential orientation at the water–air interfaces during cryo-EM based structure determination experiments and are shown to be generalizable to soluble and membrane-bound proteins.
Published in Nature Methods
Computational approaches help us explore complexities of the T cell receptor repertoire.
Published in Nature Methods
Single-objective light sheet fluorescence microscopes are driving innovation in volumetric imaging.
