Kudryashev, Mikhail Diepold, Andreas Amstutz, Marlise Armitage, Judith P Stahlberg, Henning Cornelis, Guy R
Published in
Molecular microbiology
Bacterial type III secretion systems or injectisomes are multiprotein complexes directly transporting bacterial effector proteins into eukaryotic host cells. To investigate the distribution of injectisomes in the bacterium and the influence of activation of the system on that distribution, we combined in vivo fluorescent imaging and high-resolution...
Augagneur, Yoann King, Alyssa N Germain-Amiot, Noëlla Sassi, Mohamed Fitzgerald, John W Sahukhal, Gyan S Elasri, Mohamed O Felden, Brice Brinsmade, Shaun R
Published in
Molecular microbiology
In Staphylococcus aureus, the transcription factor CodY modulates the expression of hundreds of genes, including most virulence factors, in response to the availability of key nutrients like GTP and branched-chain amino acids. Despite numerous studies examining how CodY controls gene expression directly or indirectly, virtually nothing is known abo...
Yuda, Masao Kaneko, Izumi Iwanaga, Shiroh Murata, Yuho Kato, Tomomi
Published in
Molecular microbiology
The malaria gametocyte, the gamete precursor, is the essential stage for malaria transmission to the mosquito vector. In the vertebrate host's blood, it develops into a mature male or female capable of transforming into a gamete in the mosquito blood meal. Despite the importance of this stage in the malaria life cycle, the genetic regulation of gam...
Zhang, Ruibin Slamti, Leyla Tong, Lei Verplaetse, Emilie Ma, Lixia Lemy, Christelle Peng, Qi Guo, Shuyuan Zhang, Jie Song, Fuping
...
Published in
Molecular microbiology
Cell differentiation within an isogenic population allows the specialisation of subpopulations and a division of labour. Bacillus thuringiensis is a spore-forming bacterium that produces insecticidal crystal proteins (Cry proteins) in sporulating cells. We recently reported that strain B. thuringiensis LM1212 presents the unique ability to differen...
Ates, Louis S
Published in
Molecular microbiology
The PE and PPE proteins of Mycobacterium tuberculosis have been studied with great interest since their discovery. Named after the conserved proline (P) and glutamic acid (E) residues in their N-terminal domains, these proteins are postulated to perform wide-ranging roles in virulence and immune modulation. However, technical challenges in studying...
Pu, Tianning Mei, Zhiling Zhang, Wei Liang, Wei-Jun Zhou, Xiufen Liang, Jingdan Deng, Zixin Wang, Zhijun
Published in
Molecular microbiology
Phosphorothioation (PT) involves the replacement of a nonbridging phosphate oxygen on the DNA backbone with sulfur. In bacteria, the procedure is both sequence- and stereo-specific. We reconstituted the PT reaction using purified DndCDE from Salmonella enterica and IscS from Escherichia coli. We determined that the in vitro process of PT was oxygen...
Jain, Ira Danger, Jessica L Burgess, Cameron Uppal, Timsy Sumby, Paul
Published in
Molecular microbiology
The group A Streptococcus (GAS) causes diseases that range from mild (e.g. pharyngitis) to severely invasive (e.g. necrotizing fasciitis). Strain- and serotype-specific differences influence the ability of isolates to cause individual diseases. At the center of this variability is the CovR/S two-component system and the accessory protein RocA. Thro...
Tatip, Supinda Taggart, Janet Wang, Yirong MacDiarmid, Colin W Eide, David J
Published in
Molecular microbiology
Changes in RNA are often poor predictors of protein accumulation. One factor disrupting this relationship are changes in transcription start sites (TSSs). Therefore, we explored how alterations in TSS affected expression of genes regulated by the Zap1 transcriptional activator of Saccharomyces cerevisiae. Zap1 controls their response to zinc defici...
Wang, Chu Lunelli, Michele Zschieschang, Erik Bosse, Jens Bernhard Thuenauer, Roland Kolbe, Michael
Published in
Molecular microbiology
Bacterial flagellar filaments are assembled by tens of thousands flagellin subunits, forming 11 helically arranged protofilaments. Each protofilament can take either of the two bistable forms L-type or R-type, having slightly different conformations and inter-protofilaments interactions. By mixing different ratios of L-type and R-type protofilament...
Yan, Hao Lu, Xiaorui Sun, Di Zhuang, Shuai Chen, Qiong Chen, Zhi Li, Jilun Wen, Ying
Published in
Molecular microbiology
BldD generally functions as a repressor controlling morphological development of Streptomyces. In this work, evidences that BldD also activates antibiotic production are provided. In Streptomyces roseosporus (which produces daptomycin widely used for treatment of human infections), deletion of bldD notably reduced daptomycin production, but enhance...
