Najafabadi, Zeinab Yousefi Fanuel, Songwe Falak, Reza Kaboli, Saeed Kardar, Gholam Ali
Published in
Molecular biotechnology
Biotechnological approaches have always sought to utilize novel and efficient methods in the prevention, diagnosis, and treatment of diseases. This science has consistently tried to revolutionize medical science by employing state-of-the-art technologies in genomic and proteomic engineering. CRISPR-Cas system is one of the emerging techniques in th...
Akram, Fatima Sahreen, Sania Aamir, Farheen Haq, Ikram Ul Malik, Kausar Imtiaz, Memoona Naseem, Waqas Nasir, Narmeen Waheed, Hafiza Mariam
Published in
Molecular biotechnology
Genome-editing technology has enabled scientists to make changes in model organisms' DNA at the genomic level to get biotechnologically important products from them. Most commonly employed technologies for this purpose are transcription activator like effector nucleases (TALENs), homing-endonucleases or meganucleases, zinc finger nucleases (ZFNs), ...
Ashwath, Priyanka Somanath, Disha Sannejal, Akhila Dharnappa
Published in
Molecular biotechnology
Pathogenic bacteria constitute a significant threat to mankind and at the same time represent a huge reservoir of abeyant therapeutics to prevent and treat various diseases. The concept of virulence determinants has been a compelling tool in driving research in the field of bacterial pathogenesis and infectious diseases. In this review, we highligh...
Askarian, Saeedeh Nasab, Niloofar Khandan Aghaee-Bakhtiari, Seyed Hamid Abadi, Mohammad Hassan Jafari Najaf Oskuee, Reza Kazemi
Published in
Molecular biotechnology
Selection of targeted and efficient carriers to deliver drugs and genes to cells and tissues is still a major challenge and to overcome this obstacle, aptamers conjugated to nanoparticles have been broadly examined. To assess whether polycation of aptamers can improve plasmid delivery efficacy, we investigated the effect of three DNA-aptamers (AS14...
Abula, Abudureyimu Yang, Tingting Zhang, Yingxin Li, Tinghan Ji, Xiaoyun
Published in
Molecular biotechnology
Exoribonucleases are frequently used as nuclei acids detection tools for their sequences, modifications, and structures. Escherichia coli ribonuclease R (EcR) is the prototypical exoribonuclease of the RNase II/RNB family degrading RNA in the 3'-5' direction. Different from RNase II, EcR is capable of degrading structured RNA efficiently, which mak...
Qiu, Xiangmei Zhang, Qiang Deng, Qingfang Li, Quan
Published in
Molecular biotechnology
Circular RNAs (circRNAs) have been reported to exert critical roles in human cancers. In this study, we investigated the role and molecular mechanism of hsa_circ_0012673 in breast cancer. Herein, we found that the expression of hsa_circ_0012673 was upregulated in breast cancer tissues and cell lines. Knockdown of hsa_circ_0012673 using RNA interfer...
Lv, Dingding Wang, Yuhong Li, Shiyan Shao, Xiao Jin, Qinghua
Published in
Molecular biotechnology
Lung cancer represents the most prevalent cancer worldwide and causes the death of many patients. Cancer stem cells (CSCs), a subpopulation of cancer cells, have the capacities of self-renewal, unlimited proliferation, and multiple differentiation potential. The purpose of this study was to explore the potential role of long noncoding RNA (lncRNA) ...
Saha, Ujjayani Gondi, Rashmi Patil, Amrita Saroj, Sunil D
Published in
Molecular biotechnology
The ESKAPE (Enterococcus spp., Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp.) isolates both from the clinical settings and food products are demonstrated to gain resistance to multiple antimicrobials. Therefore, the ESKAPE pathogens pose a serious threat to public health, which ...
Heo, Mi-Young Kim, Bit-Na Lee, Jaewoong Choi, Wooil Kim, Yang-Hoon Min, Jiho
Published in
Molecular biotechnology
Sharma, Achla Arif, Mian A R Shamshad, M Rawale, Kanwardeep S Brar, Anureet Burgueño, Juan Shokat, Sajid Kaur, Ravinder Vikram, Parsahnt Srivastava, Puja
...
Published in
Molecular biotechnology
Development of nutrient efficient cultivars depends on effective identification and utilization of genetic variation. We characterized a set of 276 pre-breeding lines (PBLs) for several traits at different levels of nitrogen application. These PBLs originate from synthetic wheats and landraces. We witnessed significant variation in various traits a...