Yousefzadeh, Nasibeh Jeddi, Sajad Ghiasi, Rafighe Alipour, Mohammad Reza
Published in
Molecular and cellular biochemistry
Thyroid hormone deficiency during fetal life (fetal hypothyroidism) causes intrauterine growth restriction (IUGR). Fetal hypothyroidism (FH) could attenuate normal cardiac functions in the later life of the offspring rats. The aim of this study was to evaluate the contribution of myomiR network and its target gene expression in cardiac dysfunction ...
Jovanovic, Aleksandra Obradovic, Milan Milovanovic, Emina Sudar Stewart, Alan J Pitt, Samantha J Alavantic, Dragan Aleksic, Ema Isenovic, Esma R
Published in
Molecular and cellular biochemistry
The aim of this study was to investigate whether the presence of endogenous estradiol alters the effects of a high-fat (HF) diet on activity/expression of the cardiac Na+/K+-ATPase, via PI3K/IRS and RhoA/ROCK signalling cascades in female rats. For this study, female Wistar rats (8 weeks old, 150-200 g) were fed a standard diet or a HF diet (balanc...
Schaefer, Susanne Guerra, Barbara
Published in
Molecular and cellular biochemistry
Oxygen consumption is particularly elevated in cardiac cells as they are equipped with a large number of mitochondria and high levels of respiratory chain components. Consequently, production of reactive oxygen species (ROS) is tightly controlled as an imbalance in redox reactions can lead to irreversible cellular damage. siRNA-mediated down-regula...
Luo, Jixian Li, Dingyun Wei, Dan Wang, Xiaoguang Wang, Lan Zeng, Xianlu
Published in
Molecular and cellular biochemistry
Stromal cell-derived factor-1 (SDF-1) signaling is important to the maintenance and progression of T-cell acute lymphoblastic leukemia by inducing chemotaxis migration. To identify the mechanism of SDF-1 signaling in the migration of T-ALL, Jurkat acute lymphoblastic leukemia cells were used. Results showed that SDF-1 induces Jurkat cell migration ...
Singh, Minu Mukhopadhyay, Tapas
Published in
Molecular and cellular biochemistry
Here, we have studied in p53 null H1299 lung carcinoma cells, the dominant-negative effect of human p53 (h-p53) on buffalo p53 (b-p53) induced nuclear transactivation-dependent function. Recently, we have isolated and cloned the full-length cDNA of buffalo p53. Buffalo and human p53 proteins exhibit a high degree of structural and functional simila...
Sun, Cheng Wang, Shimin Hu, Wei
Published in
Molecular and cellular biochemistry
The acid-sensing ion channel 1a (ASIC1a), which is activated by extracellular acid, contributes to the pathogenesis of rheumatoid arthritis. However, it remains unclear whether ASIC1a mediates acid-induced matrix metabolism in rat articular chondrocytes via activation of the MAPK signaling pathway. In the current study, we found that extracellular ...
Souza, Carine F Baldissera, Matheus D Bottari, Nathiele B Moreira, Karen L S da Rocha, Maria Izabel U M da Veiga, Marcelo L Santos, Roberto C V Baldisserotto, Bernardo
Published in
Molecular and cellular biochemistry
Appropriate control of the immune response is a critical determinant of fish health, and the purinergic cascade has an important role in the immune and inflammatory responses. This cascade regulates the levels of adenosine triphosphate (ATP), adenosine diphosphate, adenosine monophosphate and adenosine (Ado), molecules involved in physiological or ...
Chopra, Nisha Kaur, Jagdeep
Published in
Molecular and cellular biochemistry
In order to design proteins with improved properties i.e. thermostability, catalytic efficiency and to understand the mechanisms underlying, a thermostable variant of Bacillus lipase was generated by site-directed mutagenesis with enhanced thermal (∆Tm = + 12 °C) and chemical (∆Cm denaturation for Gdmcl = + 1.75 M) stability as compared to WT. Arg1...
Soares, Deividi C S Portela, José L R Roos, Daniel H Rodrigues, Nathane R Gomes, Karen K Macedo, Giulianna E Posser, Thais Franco, Jeferson L Hassan, Waseem Puntel, Robson L
...
Published in
Molecular and cellular biochemistry
PTZ is a convulsive agent that acts via selective blockage of GABAA receptor channels, whereas 4-AP leads to a convulsive episode via blockage of K+ channels. However, the mechanism(s) by which pentylenetetrazole (PTZ) and 4-aminopyridine (4-AP) cause toxicity to Drosophila melanogaster needs to be properly explored, once it will help in establishi...
Choi, Jaewoo Yin, Tai Shinozaki, Koichiro Lampe, Joshua W Stevens, Jan F Becker, Lance B Kim, Junhwan
Published in
Molecular and cellular biochemistry
It is commonly accepted that brain phospholipids are highly enriched with long-chain polyunsaturated fatty acids (PUFAs). However, the evidence for this remains unclear. We used HPLC-MS to analyze the content and composition of phospholipids in rat brain and compared it to the heart, kidney, and liver. Phospholipids typically contain one PUFA, such...
