Stalder, Danièle Yakunin, Igor Pereira, Conceição Eden, Jessica Gershlick, David C.
Published in
Molecular Biology of the Cell
ACBD3, a Golgi-localized protein, plays a vital role in recruiting essential proteins like PI4KIIIβ. However, how ACBD3 itself gets to the Golgi was previously unclear. Our study sheds light on a two-step mechanism for ACBD3’s Golgi recruitment. We identified a crucial MWT motif in ACBD3 and discovered a new interacting partner, SCFD1 and SEC22B, t...
Sun, Yaohui Reid, Brian Zhang, Yan Zhu, Kan Ferreira, Fernando Estrada, Alejandro Sun, Yuxin Draper, Bruce W Yue, Haicen Copos, Calina
...
Published in
Molecular biology of the cell
Recent research has elucidated mechanochemical pathways of single cell polarization, but much less is known about collective motility initiation in adhesive cell groups. We used galvanotactic assays of zebrafish keratocyte cell groups, pharmacological perturbations, electric field switches, particle imaging velocimetry, and cell tracking to show th...
Novais-Cruz, Marco Pombinho, António Sousa, Mafalda Maia, André F Maiato, Helder Ferrás, Cristina
Published in
Molecular biology of the cell
DNA damage response (DDR) during interphase involves active signaling and repair to ensure genomic stability. However, how mitotic cells respond to DNA damage remains poorly understood. Supported by correlative live-/fixed-cell microscopy, it was found that mitotic cells exposed to several cancer chemotherapy compounds acquire and signal DNA damage...
Kusumi, Akihiro Tsunoyama, Taka A Tang, Bo Hirosawa, Koichiro M Morone, Nobuhiro Fujiwara, Takahiro K Suzuki, Kenichi G N
Published in
Molecular biology of the cell
Two very polarized views exist for understanding the cellular plasma membrane (PM). For some, it is the simple fluid described by the original Singer-Nicolson fluid mosaic model. For others, due to the presence of thousands of molecular species that extensively interact with each other, the PM forms various clusters and domains that are constantly ...
van den Berg, Sebastiaan J W East, Samuel Mitra, Sreyoshi Jansen, Lars E T
Published in
Molecular biology of the cell
The centromere is a unique chromatin domain that links sister chromatids and forms the attachment site for spindle microtubules in mitosis. Centromere inheritance is largely DNA sequence-independent but strongly reliant on a self-propagating chromatin domain featuring nucleosomes containing the H3 variant CENP-A. Unlike other histones, CENP-A is ma...
Cabrera, Alejandra J H Gumbiner, Barry M Kwon, Young V
Published in
Molecular biology of the cell
Given the role of E-cadherin (E-cad) in holding epithelial cells together, an inverse relationship between E-cad levels and cell invasion during the epithelial-mesenchymal transition and cancer metastasis has been well recognized. Here we report that E-cad is necessary for the invasiveness of RasV12-transformed intestinal epithelial cells in Drosop...
Gunkel, Philip Iino, Haruki Krull, Sandra Cordes, Volker C
Published in
Molecular biology of the cell
The proteins ZC3HC1 and TPR are structural components of the nuclear basket (NB), a fibrillar structure attached to the nucleoplasmic side of the nuclear pore complex (NPC). ZC3HC1 initially binds to the NB in a TPR-dependent manner and can subsequently recruit additional TPR polypeptides to this structure. Here, we examined the molecular propertie...
Mfarej, Michael G. Hyland, Caitlin A. Sanchez, Annie C. Falk, Matthias M. Iovine, M. Kathryn Skibbens, Robert V.
Published in
Molecular Biology of the Cell
Cohesins are ATPase complexes that play central roles in cellular processes such as chromosome division, DNA repair, and gene expression. Cohesinopathies arise from mutations in cohesin proteins or cohesin complex regulators and encompass a family of related developmental disorders that present with a range of severe birth defects, affect many diff...
Kochendoerfer, Antje M. Keegan, Rachel S. Dunleavy, Elaine M.
Published in
Molecular Biology of the Cell
Stem cells can undergo asymmetric cell division (ACD) giving rise to one new stem cell and one differentiating daughter cell. In Drosophila germline stem cells (GSCs), the centromeric histone CENP-A (CID in flies) is asymmetrically distributed between sister chromatids such that chromosomes that end up in the GSC harbor more CID at centromeres. A m...
Cordova-Burgos, Luigy Rao, Deepti Egwuonwu, Joshua Borinskaya, Sofya Sasidharan, Shashikala Soto, Martha
Published in
Molecular Biology of the Cell
Cadherin dynamics drive morphogenesis, while defects in cadherin polarity contribute to diseases, including cancers. However, the forces polarizing cadherin membrane distribution are not well understood. We previously showed that WAVE-dependent branched actin polarizes cadherin distribution and suggested that one mechanism is protein transport. Whi...
