Delafield, Daniel G Li, Lingjun
Published in
Molecular & cellular proteomics : MCP
Growing implications of glycosylation in physiological occurrences and human disease have prompted intensive focus on revealing glycomic perturbations through absolute and relative quantification. Empowered by seminal methodologies and increasing capacity for detection, identification, and characterization, the past decade has provided a significan...
Zhang, Yong Zhao, Wanjun Mao, Yonghong Chen, Yaohui Wang, Shisheng Zhong, Yi Su, Tao Gong, Meng Du, Dan Lu, Xiaofeng
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Molecular & cellular proteomics : MCP
The glycoprotein spike (S) on the surface of SARS-CoV-2 is a determinant for viral invasion and host immune response. Herein, we characterized the site-specific N-glycosylation of S protein at the level of intact glycopeptides. All 22 potential N-glycosites were identified in the S-protein protomer and were found to be preserved among the 753 SARS-...
West, Christopher M Malzl, Daniel Hykollari, Alba Wilson, Iain B H
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Molecular & cellular proteomics : MCP
Glycosylation is a highly diverse set of co- and posttranslational modifications of proteins. For mammalian glycoproteins, glycosylation is often site-, tissue-, and species-specific and diversified by microheterogeneity. Multitudinous biochemical, cellular, physiological, and organismic effects of their glycans have been revealed, either intrinsic...
Pepi, Lauren E Sanderson, Patience Stickney, Morgan Amster, I Jonathan
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Molecular & cellular proteomics : MCP
This review covers recent developments in glycosaminoglycan (GAG) analysis via mass spectrometry (MS). GAGs participate in a variety of biological functions, including cellular communication, wound healing, and anticoagulation, and are important targets for structural characterization. GAGs exhibit a diverse range of structural features due to the ...
Hackett, William E Zaia, Joseph
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Molecular & cellular proteomics : MCP
Complex protein glycosylation occurs through biosynthetic steps in the secretory pathway that create macro- and microheterogeneity of structure and function. Required for all life forms, glycosylation diversifies and adapts protein interactions with binding partners that underpin interactions at cell surfaces and pericellular and extracellular envi...
Porsdam Mann, Sebastian Treit, Peter V Geyer, Philipp Emanuel Omenn, Gilbert S Mann, Matthias
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Molecular & cellular proteomics : MCP
Recent advances in MS-based proteomics have vastly increased the quality and scope of biological information that can be derived from human samples. These advances have rendered current workflows increasingly applicable in biomedical and clinical contexts. As proteomics is poised to take an important role in the clinic, associated ethical responsib...
Kurz, Simone Sheikh, M Osman Lu, Shan Wells, Lance Tiemeyer, Michael
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Molecular & cellular proteomics : MCP
High performance liquid chromatography has been employed for decades to enhance detection sensitivity and quantification of complex analytes within biological mixtures. Among these analytes, glycans released from glycoproteins and glycolipids have been characterized as underivatized or fluorescently tagged derivatives by HPLC coupled to various det...
Zhu, Wenhe Jiang, Xiaoming Sun, Hongyu Li, Yawei Shi, Wenyan Zheng, Meiyu Liu, Di Ma, Aixin Feng, Xianmin
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Molecular & cellular proteomics : MCP
Giardia lamblia (G. lamblia) disease is a zoonosis with a-infection rate affecting the general population of the world. Despite the constant possibility of damage due to their own metabolism, G. lamblia have survived and evolved to adapt to various environments. However, research on energy-metabolism conversion in G. lamblia is limited. This study ...
Keser, Toma Tijardović, Marko Gornik, Ivan Lukic, Edita Lauc, Gordan Gornik, Olga Novokmet, Mislav
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Molecular & cellular proteomics : MCP
Alpha-1-acid glycoprotein (AGP) is an acute phase glycoprotein in blood, which is primarily synthetized in the liver and whose biological role is not completely understood. It consists of 45% carbohydrates that are present in the form of five N-linked complex glycans. AGP N-glycosylation was shown to be changed in many different diseases and some c...
Struk, Sylwia De Cuyper, Carolien Jacobs, Anse Braem, Lukas Walton, Alan De Keyser, Annick Depuydt, Stephen Vu, Lam Dai De Smet, Ive Boyer, François-Didier
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Molecular & cellular proteomics : MCP
The F-box protein MORE AXILLARY GROWTH 2 (MAX2) is a central component in the signaling cascade of strigolactones (SLs) as well as of the smoke derived karrikins (KARs) and the so far unknown endogenous KAI2 ligand (KL). The two groups of molecules are involved in overlapping and unique developmental processes, and signal-specific outcomes are attr...