Pyke, Rachel Marty Mellacheruvu, Datta Dea, Steven Abbott, Charles Zhang, Simo V Phillips, Nick A Harris, Jason Bartha, Gabor Desai, Sejal McClory, Rena
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Molecular & cellular proteomics : MCP
Major histocompatibility complex (MHC)-bound peptides that originate from tumor-specific genetic alterations, known as neoantigens, are an important class of anti-cancer therapeutic targets. Accurately predicting peptide presentation by MHC complexes is a key aspect of discovering therapeutically relevant neoantigens. Technological improvements in ...
Burnap, Sean A Mayr, Ursula Shankar-Hari, Manu Cuello, Friederike Thomas, Mark R Shah, Ajay M Sabroe, Ian Storey, Robert F Mayr, Manuel
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Molecular & cellular proteomics : MCP
We have previously shown that multimers of plasma pentraxin-3 (PTX3) were predictive of survival in patients with sepsis. To characterize the release kinetics and cellular source of plasma protein changes in sepsis, serial samples were obtained from healthy volunteers (n = 10; three time points) injected with low-dose endotoxin (lipopolysaccharide ...
Delafield, Daniel G Li, Lingjun
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Molecular & cellular proteomics : MCP
Growing implications of glycosylation in physiological occurrences and human disease have prompted intensive focus on revealing glycomic perturbations through absolute and relative quantification. Empowered by seminal methodologies and increasing capacity for detection, identification, and characterization, the past decade has provided a significan...
Zhang, Yong Zhao, Wanjun Mao, Yonghong Chen, Yaohui Wang, Shisheng Zhong, Yi Su, Tao Gong, Meng Du, Dan Lu, Xiaofeng
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Molecular & cellular proteomics : MCP
The glycoprotein spike (S) on the surface of SARS-CoV-2 is a determinant for viral invasion and host immune response. Herein, we characterized the site-specific N-glycosylation of S protein at the level of intact glycopeptides. All 22 potential N-glycosites were identified in the S-protein protomer and were found to be preserved among the 753 SARS-...
Qi, Huan Ma, Mingliang Hu, Chuansheng Xu, Zhao-Wei Wu, Fan-Lin Wang, Nan Lai, Dan-Yun Li, Yang Zhang, Hainan Jiang, He-Wei
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Molecular & cellular proteomics : MCP
Antibodies play essential roles in both diagnostics and therapeutics. Epitope mapping is essential to understand how an antibody works and to protect intellectual property. Given the millions of antibodies for which epitope information is lacking, there is a need for high-throughput epitope mapping. To address this, we developed a strategy, Antibod...
Goodman, Craig A Davey, Jonathan R Hagg, Adam Parker, Benjamin L Gregorevic, Paul
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Molecular & cellular proteomics : MCP
Ubiquitination is a posttranslational protein modification that has been shown to have a range of effects, including regulation of protein function, interaction, localization, and degradation. We have previously shown that the muscle-specific ubiquitin E3 ligase, ASB2β, is downregulated in models of muscle growth and that overexpression ASB2β is su...
Zhang, Xinting Chang, Yuyang Zhai, Wanying Qian, Feng Zhang, Yingqing Xu, Shumei Guo, Haiyan Wang, Siyu Hu, Ruiqin Zhong, Xiaozhu
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Molecular & cellular proteomics : MCP
Gonadal soma-derived factor (gsdf) has been demonstrated to be essential for testicular differentiation in medaka (Oryzias latipes). To understand the protein dynamics of Gsdf in spermatogenesis regulation, we used a His-tag "pull-down" assay coupled with shotgun LC-MS/MS to identify a group of potential interacting partners for Gsdf, which include...
Pepi, Lauren E Sanderson, Patience Stickney, Morgan Amster, I Jonathan
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Molecular & cellular proteomics : MCP
This review covers recent developments in glycosaminoglycan (GAG) analysis via mass spectrometry (MS). GAGs participate in a variety of biological functions, including cellular communication, wound healing, and anticoagulation, and are important targets for structural characterization. GAGs exhibit a diverse range of structural features due to the ...
West, Christopher M Malzl, Daniel Hykollari, Alba Wilson, Iain B H
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Molecular & cellular proteomics : MCP
Glycosylation is a highly diverse set of co- and posttranslational modifications of proteins. For mammalian glycoproteins, glycosylation is often site-, tissue-, and species-specific and diversified by microheterogeneity. Multitudinous biochemical, cellular, physiological, and organismic effects of their glycans have been revealed, either intrinsic...
Fang, Kailun Li, Qidan Wei, Yu Zhou, Changyang Guo, Wenhui Shen, Jiaqi Wu, Ruoxi Ying, Wenqin Yu, Lu Zi, Jin
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Molecular & cellular proteomics : MCP
The molecular mechanism associated with mammalian meiosis has yet to be fully explored, and one of the main reasons for this lack of exploration is that some meiosis-essential genes are still unknown. The profiling of gene expression during spermatogenesis has been performed in previous studies, yet few studies have aimed to find new functional gen...
