Khanam, Salma Funatsu, Takayuki Tanaka, Koji Kaneko, Yasuko
Published in
Microscopy (Oxford, England)
Lipid droplets and membranes in radicle cells from desiccated embryonic axes of soybean (Glycine max) seeds were examined by a recently developed correlative light and electron microscopy system, which has been designed to facilitate the observation of identical locations using an upright reflected light microscope and compact SEM successively with...
Yahagi, Tsukaho
Published in
Microscopy (Oxford, England)
The image contrast obtained in electron microscopy depends on the atomic number of the sample. Therefore, obtaining a clear contrast is challenging when samples composed of light elements (carbon materials and polymers) are embedded in the resin. Herein, a newly developed embedding composition exhibiting low viscosity and high electron density is r...
Sheikh, Aadil Zechmann, Bernd Sayes, Christie M Taube, Joseph H Greathouse, K Leigh
Published in
Microscopy (Oxford, England)
Biological nanoparticles, such as bacterial outer membrane vesicles (OMVs), are routinely characterized through transmission electron microscopy (TEM). In this study, we report a novel method to prepare OMVs for TEM imaging. To preserve vesicular shape and structure, we developed a dual fixation protocol involving osmium tetroxide incubation prior ...
Sasaki, Yusei Yamamoto, Kazuo Anada, Satoshi Yoshimoto, Noriyuki
Published in
Microscopy (Oxford, England)
To improve the performance of organic light-emitting diodes (OLEDs), it is essential to understand and control the electric potential in the organic semiconductor layers. Electron holography (EH) is a powerful technique for visualizing the potential distribution with a transmission electron microscope. However, it has a serious issue that high-ener...
Pivak, Yevheniy Park, Junbeom Basak, Shibabrata Eichel, Rüdiger-Albert Beker, Anne Rozene, Alejandro Pérez Garza, Héctor Hugo Sun, Hongyu
Published in
Microscopy (Oxford, England)
Liquid-phase transmission electron microscopy (LPTEM) technique has been used to perform a wide range of in situ and operando studies. While most studies are based on the sample contrast change in the liquid, acquiring high qualitative results in the native liquid environment still poses a challenge. Herein, we present a novel and facile method to ...
Grimm, Tyler J Mears, Laine
Published in
Microscopy (Oxford, England)
Electrically assisted heat treatment is the process of applying an electric current to a sample during heat treatment. Literature has generally shown there to be a difference in the resulting effects of direct current (DC) current and highly transient current (i.e. electropulsing). However, these differences are poorly characterized. In situ transm...
Nakazawa, Katsuaki Mitsuishi, Kazutaka
Published in
Microscopy (Oxford, England)
Diffraction patterns contain useful information about the materials. Recent developments in four-dimensional scanning transmission electron microscopy and the acquisition of the spatial distribution of diffraction patterns have produced significant results. The acquisition of a temporal series of diffractions is achieved for a stationary beam. Howe...
Enyama, Momoyo Nishi, Ryuji Ito, Hiroyuki Yamasaki, Jun
Published in
Microscopy (Oxford, England)
To suppress aberrations in the signal electron optics of a scanning electron microscope, we propose ExB deflector (deflector with superimposed electric and magnetic fields) optics that cancel the aberrations generated during large-angle deflection. This improves the resolution of the angle or position of the signal electrons on the sample surface, ...
Tanida, Isei Yamaguchi, Junji Suzuki, Chigure Kakuta, Soichiro Uchiyama, Yasuo
Published in
Microscopy (Oxford, England)
Correlative fluorescent and electron microscopic images of the same section of epoxy (or other polymer)-embedded samples, hereafter referred to as 'in-resin CLEM', have been developed to improve the positional accuracy and Z-axis resolution limitations of conventional correlative light and electron microscopy (CLEM). High-pressure freezing and quic...
Vandenabeele, Frank Stevens, Sjoerd Snijders, Tim Stessel, Björn Dubois, Jasperina van Loon, Luc J C Lambrichts, Ivo Agten, Anouk
Published in
Microscopy (Oxford, England)
Patients infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) who have been admitted to the intensive care unit (ICU) often face months of physical disability after discharge. To optimize recovery, it is important to understand the role of musculoskeletal alterations in critically ill patients infected with SARS-CoV-2. The mai...
