Mouhib, Mohammed Chi, Celestine N
Published in
MicrobiologyOpen
Gaining structural information on membrane proteins in their native lipid environment is a long-standing challenge in molecular biology. Instead, it is common to employ membrane mimetics, which has been shown to affect protein structure, dynamics, and function severely. Here, we describe the incorporation of a bacterial outer membrane protein (OmpW...
Swanner, Elizabeth D Wüstner, Marina Leung, Tania Pust, Jürgen Fatka, Micah Lambrecht, Nick Chmiel, Hannah E Strauss, Harald
Published in
MicrobiologyOpen
Subsurface chlorophyll maxima layers (SCML) are ubiquitous features of stratified aquatic systems. Availability of the micronutrient iron is known to influence marine SCML, but iron has not been explored in detail as a factor in the development of freshwater SCML. This study investigates the relationship between dissolved iron and the SCML within t...
Pietsch, Grace M Gazis, Romina Klingeman, William E Huff, Matthew L Staton, Margaret E Kolarik, Miroslav Hadziabdic, Denita
Published in
MicrobiologyOpen
Symbioses between Geosmithia fungi and wood-boring and bark beetles seldom result in disease induction within the plant host. Yet, exceptions exist such as Geosmithia morbida, the causal agent of Thousand Cankers Disease (TCD) of walnuts and wingnuts, and Geosmithia sp. 41, the causal agent of Foamy Bark Canker disease of oaks. Isolates of G. obscu...
Goussarov, Gleb Mysara, Mohamed Vandamme, Peter Van Houdt, Rob
Published in
MicrobiologyOpen
The rise of metagenomics offers a leap forward for understanding the genetic diversity of microorganisms in many different complex environments by providing a platform that can identify potentially unlimited numbers of known and novel microorganisms. As such, it is impossible to imagine new major initiatives without metagenomics. Nevertheless, it r...
Najafpour, Babak Pinto, Patricia I S Canario, A V M Power, Deborah M
Published in
MicrobiologyOpen
The goal of this study was to design genus-specific primers for rapid evaluation of the most abundant bacterial genera identified using amplicon-based sequencing of the 16S rRNA gene in fish-related samples and surrounding water. Efficient genus-specific primers were designed for 11 bacterial genera including Alkalimarinus, Colwellia, Enterovibrio,...
Ai, Yi Luo, Tongyu Yu, Yao Zhou, Jungang Lu, Hong
Published in
MicrobiologyOpen
The growth and tolerance of Kluyveromyces marxianus at high temperatures decreased significantly in the synthetic medium (SM), which is commonly used in industrial fermentations. After 100 days of adaptive laboratory evolution, a strain named KM234 exhibited excellent tolerance at a high temperature, without loss of its growth ability at a moderate...
Ohtsuka, Hokuto Imada, Kazuki Shimasaki, Takafumi Aiba, Hirofumi
Published in
MicrobiologyOpen
The fission yeast Schizosaccharomyces pombe employs two main strategies to adapt to the environment and survive when starved for nutrients. The strategies employ sporulation via sexual differentiation and extension of the chronological lifespan. When a cell is exposed to nutrient starvation in the presence of a cell of the opposite sex, the cells u...
Steinke, Nicola Vidal-Melgosa, Silvia Schultz-Johansen, Mikkel Hehemann, Jan-Hendrik
Published in
MicrobiologyOpen
Marine algae drive the marine carbon cycle, converting carbon dioxide into organic material. A major component of this produced biomass is a variety of glycans. Marine α-glucans include a range of storage glycans from red and green algae, bacteria, fungi, and animals. Although these compounds are likely to account for a high amount of the carbon st...
Kropp, Cosimo Lipp, Julius Schmidt, Anna Lena Seisenberger, Christina Linde, Mona Hinrichs, Kai-Uwe Babinger, Patrick
Published in
MicrobiologyOpen
As a hallmark of Archaea, their cell membranes are comprised of ether lipids. However, Archaea-type ether lipids have recently been identified in Bacteria as well, with a somewhat different composition: In Bacillales, sn-glycerol 1-phosphate is etherified with one C35 isoprenoid chain, which is longer than the typical C20 chain in Archaea, and inst...
Cho, Ji Sung Oh, Hye Ji Jang, Young Eun Kim, Hyun Jin Kim, Areum Song, Jong-Am Lee, Eun Jung Lee, Jeewon
Published in
MicrobiologyOpen
Heterologous protein production in Saccharomyces cerevisiae is a useful and effective strategy with many advantages, including the secretion of proteins that require posttranslational processing. However, heterologous proteins in S. cerevisiae are often secreted at comparatively low levels. To improve the production of the heterologous protein, hum...
