Delaunay, Tiphaine Achard, Carole Grégoire, Marc Tangy, Frédéric Boisgerault, Nicolas Fonteneau, Jean-François
Published in
Methods in molecular biology (Clifton, N.J.)
Oncolytic immunotherapy efficacy relies partially on the induction of immunogenic tumor cell death following infection with oncolytic viruses (OV) to induce an antitumor immune response. Here, we describe a method to determine if an OV is able to induce such an immunogenic tumor cell death. This method consists in testing whether tumor cells lysed ...
Meilhoc, E Teissie, J
Published in
Methods in molecular biology (Clifton, N.J.)
Intact yeast cell transformation is easily achieved by gene electrotransfer (GET). The procedure is fast and efficient in terms of transformants/μg DNA. Yeast cells in exponential growth phase are washed, treated for a short period with dithiothreitol (DTT) and then mixed with the plasmid DNA in a buffer with a low conductivity. A single well defin...
Cruz, Pamela Lemos Restrepo, María Isabel de Bernonville, Thomas Dugé Oudin, Audrey Munsch, Thibaut Lanoue, Arnaud Besseau, Sébastien Atehortùa, Lucia Giglioli-Guivarc'h, Nathalie Papon, Nicolas
...
Published in
Methods in molecular biology (Clifton, N.J.)
Monoterpene indole alkaloids (MIAs) are specialized metabolites synthesized in many plants of the Apocynaceae family including Catharanthus roseus and Rauvolfia sp. MIAs are part of the chemical arsenal that plants evolved to face pet and herbivore attacks, and their high biological activities also confer pharmaceutical properties exploited in huma...
Goldie, Kenneth N Abeyrathne, Priyanka Kebbel, Fabian Chami, Mohamed Ringler, Philippe Stahlberg, Henning
Published in
Methods in molecular biology (Clifton, N.J.)
Electron crystallography is used to study membrane proteins in the form of planar, two-dimensional (2D) crystals, or other crystalline arrays such as tubular crystals. This method has been used to determine the atomic resolution structures of bacteriorhodopsin, tubulin, aquaporins, and several other membrane proteins. In addition, a large number of...
Arheit, Marcel Castaño-Diéz, Daniel Thierry, Raphaël Abeyrathne, Priyanka Gipson, Bryant R Stahlberg, Henning
Published in
Methods in molecular biology (Clifton, N.J.)
Electron crystallography of membrane proteins uses cryo-transmission electron microscopy to record images and diffraction patterns of frozen-hydrated 2D crystals. Each two-dimensional (2D) crystal is only imaged once, at one specific tilt angle, and the recorded images can be automatically processed with the 2dx/MRC software package. Processed imag...
Arheit, Marcel Castaño-Díez, Daniel Thierry, Raphaël Gipson, Bryant R Zeng, Xiangyan Stahlberg, Henning
Published in
Methods in molecular biology (Clifton, N.J.)
Electron crystallography of membrane proteins uses cryo-transmission electron microscopy to image frozen-hydrated 2D crystals. The processing of recorded images exploits the periodic arrangement of the structures in the images to extract the amplitudes and phases of diffraction spots in Fourier space. However, image imperfections require a crystal ...
Arheit, Marcel Castaño-Díez, Daniel Thierry, Raphaël Gipson, Bryant R Zeng, Xiangyan Stahlberg, Henning
Published in
Methods in molecular biology (Clifton, N.J.)
Electron crystallography of membrane proteins records images and diffraction patterns of frozen-hydrated two-dimensional (2D) crystals. To reconstruct the high-resolution three-dimensional (3D) structure of a membrane protein, a multitude of images of 2D crystals have to be processed. Certain processing steps are thereby similar for batches of imag...
Su, Xuan Shen, Yan Weintraub, Neal L Tang, Yaoliang
Published in
Methods in molecular biology (Clifton, N.J.)
Stem cell-based therapies hold great promise as alternative therapeutic strategies for various chronic diseases, including ischemic cardiomyopathy. Tracking the engraftment of transplanted stem cells is critical to the assessment of donor cell survival in the host environment. Fluorescent proteins, such as green fluorescent protein (GFP), have been...
Han, Sang-Soo Kang, Sun-Woong
Published in
Methods in molecular biology (Clifton, N.J.)
Stem cell therapy offers promising solutions to diseases and injuries that traditional medicines and therapies can't effectively cure. To get and explain their full therapeutic potentials, the survival, viability, integration, homing, and differentiation of stem cells after transplant must be clearly understood. To meet these urgent needs, noninvas...
Muranaka, Tomoaki Oyama, Tokitaka
Published in
Methods in molecular biology (Clifton, N.J.)
The bioluminescent reporter system is a powerful tool for the long-term monitoring of gene expression because of its noninvasive nature. Furthermore, in combination with high-sensitive imaging technology, spatiotemporal analysis on regulation and heterogeneity in gene expression is possible. We developed a single-cell bioluminescent imaging system ...
