Fang, Ziyan Lagier, Margaux Méresse, Stéphane
Published in
Methods in molecular biology (Clifton, N.J.)
Salmonella enterica is a Gram-negative intracellular pathogen that causes a range of life-threatening diseases in humans and animals worldwide. In a systemic infection, the ability of Salmonella to survive/replicate in macrophages, particularly in the liver and spleen, is crucial for virulence. Transformed macrophage cell lines and primary macropha...
Piazza, Aurèle Rajput, Pallavi Heyer, Wolf-Dietrich
Published in
Methods in molecular biology (Clifton, N.J.)
DNA double-strand breaks (DSBs) are genotoxic lesions that can be repaired in a templated fashion by homologous recombination (HR). HR is a complex pathway that involves the formation of DNA joint molecules (JMs) containing heteroduplex DNA. Various types of JMs are formed throughout the pathway, including displacement loops (D-loops), multi-invasi...
Vugic, Domagoj Ehlén, Åsa Carreira, Aura
Published in
Methods in molecular biology (Clifton, N.J.)
DNA double-strand breaks (DSBs) are among the most toxic lesions. This type of DNA damage is repaired by two major pathways, homologous recombination (HR), operating only in S/G2 cell-cycle phases and nonhomologous end joining (NHEJ) which is operative throughout the cell cycle. Because HR is a template-directed repair, it is generally less prone t...
Arnould, Coline Rocher, Vincent Legube, Gaëlle
Published in
Methods in molecular biology (Clifton, N.J.)
Among the types of damage, DNA double-strand breaks (DSBs) (provoked by various environmental stresses, but also during normal cell metabolic activity) are the most deleterious, as illustrated by the variety of human diseases associated with DSB repair defects. DSBs are repaired by two groups of pathways: homologous recombination (HR) and nonhomolo...
Gemble, Simon Amor-Guéret, Mounira
Published in
Methods in molecular biology (Clifton, N.J.)
Ultrafine anaphase bridges (UFBs) result from a defect in sister chromatid segregation during anaphase. They arise from particular DNA structures, mostly generated at specific loci in the human genome, such as centromeres, common fragile sites, telomeres, or ribosomal DNA. Increases in UFB frequency are a marker of genetic instability, and their de...
Hinze, Claudia McGourty, Kieran Boucrot, Emmanuel
Published in
Methods in molecular biology (Clifton, N.J.)
Quiescence (also called "G0") is the state in which cells have exited the cell cycle but are capable to reenter as required. Though poorly understood, it represents one of the most prevalent cell states across all life. Many biologically important cell types reside in quiescence including mature hepatocytes, endothelial cells, and dormant adult ste...
Johnson, Jennifer L Pestonjamasp, Kersi Kiosses, William B Catz, Sergio D
Published in
Methods in molecular biology (Clifton, N.J.)
Neutrophils are short-lived cells after isolation. The analysis of neutrophil vesicular trafficking requires rapid and gentle handling. Recently developed super-resolution microscopy technologies have generated unparalleled opportunities to help understand the molecular mechanisms regulating neutrophil vesicular trafficking, exocytosis, and associa...
Mao, Ying Qing Petritis, Brianne Huang, Ruo-Pan
Published in
Methods in molecular biology (Clifton, N.J.)
Sandwich-based antibody arrays enable the detection of multiple proteins simultaneously, thus offering a time- and cost-effective alternative to single-plex platforms. The protein of interest is "sandwiched" between an antibody that captures it to the array and a second antibody that is used for detection. Here we describe a 1-day procedure to proc...
Tang, Hao Duan, Chaohui Kuang, Zhizhou Huang, Ruo-Pan
Published in
Methods in molecular biology (Clifton, N.J.)
Cell signaling is comprised of complex networks that regulate homeostasis and human diseases. The analyses of such pathways would improve our understanding of disease pathology and direct drug development. However, it remains a great challenge to study pathways using traditional methods. We developed a high-throughput sandwich-based antibody array ...
Owens, Joshua A Jones, Rheinallt M
Published in
Methods in molecular biology (Clifton, N.J.)
Gut mucosal immune cells play an essential role in health due to their ability to orchestrate host signaling events in response to exogenous antigens. These antigens may originate from microorganisms including viruses, commensal or pathogenic bacteria, or single-celled eukaryotes, as well as from dietary foodstuff-derived proteins or products. A cr...
