Muranaka, Tomoaki Oyama, Tokitaka
Published in
Methods in molecular biology (Clifton, N.J.)
The bioluminescent reporter system is a powerful tool for the long-term monitoring of gene expression because of its noninvasive nature. Furthermore, in combination with high-sensitive imaging technology, spatiotemporal analysis on regulation and heterogeneity in gene expression is possible. We developed a single-cell bioluminescent imaging system ...
Zhang, Linwen Allworth, Linda L Vertes, Akos
Published in
Methods in molecular biology (Clifton, N.J.)
Non-targeted metabolic analysis of single cells by mass spectrometry (MS) is important for understanding individual cell functions and characterizing cell-to-cell heterogeneity. However, identifying biomolecules in single cells presents significant challenges due to the low picoliter volume samples and the structural diversity of metabolites. Capil...
Le, Phuong Chitoor, Shweta Tu, Chunlai Lim, Sung Jun Smith, Andrew M
Published in
Methods in molecular biology (Clifton, N.J.)
In this chapter, we describe the preparation of fluorescent quantum dots for imaging and measuring protein expression in cells. Quantum dots are nanocrystals that have numerous advantages for biomolecular detection compared with organic dyes and fluorescent proteins, but their large size has been a limiting factor. We describe the synthesis of nano...
Shrestha, Bindesh
Published in
Methods in molecular biology (Clifton, N.J.)
Information on cellular metabolism at the single-cell level can unravel countless biochemical process providing invaluable biomedical insight. Single-cell analysis field is at the very early stage at this moment, and all the work done so far are proof-of-principle work by early-stage researchers. In this chapter, I have outlined ten fundamental iss...
Kreth, Jens Abdelrahman, Yasser M Merritt, Justin
Published in
Methods in molecular biology (Clifton, N.J.)
Similar to other mucosal surfaces of the body, the oral cavity hosts a diverse microbial flora that live in polymicrobial biofilm communities. It is the ecology of these communities that are the primary determinants of oral health (symbiosis) or disease (dysbiosis). As such, both symbiosis and dysbiosis are inherently polymicrobial phenomena. In an...
Caridha, Diana Leed, Susan Cawlfield, Alicia
Published in
Methods in molecular biology (Clifton, N.J.)
Confirming the in vivo efficacy of potential antileishmanial compounds that display in vitro potency and good chemical characteristics is one of the most important steps in preclinical research drug discovery before human clinical trials begin. Here we describe the use of the in vivo bioluminescent monitoring of high and low inocula of luciferase-e...
Ferreira, Christina R Pirro, Valentina Jarmusch, Alan K Alfaro, Clint M Cooks, R Graham
Published in
Methods in molecular biology (Clifton, N.J.)
Desorption electrospray ionization (DESI) is a spray-based ambient ionization method for mass spectrometry (MS) that generates ions in native atmospheric conditions (e.g., pressure and temperature). Ambient ionization allows in situ analysis of unmodified samples by eliminating analyte extraction and separation steps before MS. Lipid analysis of in...
Misra, Biswapriya B
Published in
Methods in molecular biology (Clifton, N.J.)
In this age of -omics data-guided big data revolution, metabolomics has received significant attention as compared to genomics, transcriptomics, and proteomics for its proximity to the phenotype, the promises it makes and the challenges it throws. Although metabolomes of entire organisms, organs, biofluids, and tissues are of immense interest, a ce...
Krismer, Jasmin Sobek, Jens Steinhoff, Robert F Brönnimann, Rolf Pabst, Martin Zenobi, Renato
Published in
Methods in molecular biology (Clifton, N.J.)
Mass spectrometry based metabolomics is the highly multiplexed, label-free analysis of small molecules such as metabolites or lipids in biological systems, and thus one of the most direct ways to characterize phenotypes. However, the phenotyping of populations with single-cell resolution is a great challenge due to the small number of molecules con...
Li, Xiang Li, Minyong
Published in
Methods in molecular biology (Clifton, N.J.)
Bioluminescent imaging (BLI) technology has been extensively applied due to various advantages such as noninvasiveness, high sensitivity and selectivity, excellent biocompatibility and real-time visualization and monitoring. The firefly luciferase (Fluc)/luciferin system, one of the principal bioluminescent systems, has been developed as a sensor f...
