Cui, Jian Lan, Zirui Liu, Yisi Li, Ruilin Li, Fan Sourina, Olga Müller-Wittig, Wolfgang
Published in
Methods (San Diego, Calif.)
Driver drowsiness is one of the main factors leading to road fatalities and hazards in the transportation industry. Electroencephalography (EEG) has been considered as one of the best physiological signals to detect drivers' drowsy states, since it directly measures neurophysiological activities in the brain. However, designing a calibration-free s...
Jingxin, Liu Mengchao, Zhang Yuchen, Liu Jinglei, Cui Yutong, Zhong Zhong, Zhang Lihui, Zu
Published in
Methods (San Diego, Calif.)
In this paper, we utilized deep learning methods to screen the positive COVID-19 cases in chest CT. Our primary goal is to supply rapid and precise assistance for disease surveillance on the medical imaging aspect. Basing on deep learning, we combined semantic segmentation and object detection methods to study the lesion performance of COVID-19. We...
Falak, Samreen Macdonald, Rainer Busby, Eloise J O'Sullivan, Denise M Milavec, Mojca Plauth, Annabell Kammel, Martin Zeichhardt, Heinz Grunert, Hans-Peter Kummrow, Andreas
...
Published in
Methods (San Diego, Calif.)
Viral load monitoring in human immunodeficiency virus type 1 (HIV-1) infection is often performed using reverse transcription quantitative PCR (RT-qPCR) to observe response to treatment and identify the development of resistance. Traceability is achieved using a calibration hierarchy traceable to the International Unit (IU). IU values are determine...
Long, Samuel
Published in
Methods (San Diego, Calif.)
Sensitive PCR detection of viral nucleic acids plays a critical role in infectious disease research, diagnosis and monitoring. In the context of SARS-CoV-2 detection, recent reports indicate that digital PCR-based tests are significantly more sensitive than traditional qPCR tests. Numerous factors can influence digital PCR reaction sensitivity. In ...
Whale, Alexandra S von der Heide, Eva K Kohlenberg, Max Brinckmann, Anja Baedker, Silke Karalay, Oezlem Fernandez-Gonzalez, Ana Busby, Eloise J Bustin, Stephen A Hauser, Heiko
...
Published in
Methods (San Diego, Calif.)
Coronavirus disease 2019 (COVID-19) is an infectious, acute respiratory disease caused mainly by person-to-person transmission of the coronavirus SARS-CoV-2. Its emergence has caused a world-wide acute health crisis, intensified by the challenge of reliably identifying individuals likely to transmit the disease. Diagnosis is hampered by the many un...
van Snippenberg, Willem Gleerup, David Rutsaert, Sofie Vandekerckhove, Linos De Spiegelaere, Ward Trypsteen, Wim
Published in
Methods (San Diego, Calif.)
The development of an HIV-1 cure is hampered by the existence of a persistent (latent) reservoir that contains a small proportion of replication-competent intact proviruses which refuels viral replication upon treatment discontinuation. Therefore, an accurate evaluation and quantification of these (intact) proviruses is essential to determine the e...
Milavec, Mojca Pavšič, Jernej Bogožalec Košir, Alexandra Jones, Gerwyn M O'Sullivan, Denise M Devonshire, Alison S Van Heuverswyn, Fran Karczmarczyk, Maria Neeb, Jannika Plauth, Annabell
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Published in
Methods (San Diego, Calif.)
A candidate digital PCR (dPCR)-based reference measurement procedure for quantification of human cytomegalovirus (hCMV) was evaluated in 10 viral load comparison schemes (seven external quality assessment (EQA) and three additional training schemes) organized by INSTAND e.V. over four years (between September 2014 and March 2018). Four metrology in...
Long, Samuel Berkemeier, Brian
Published in
Methods (San Diego, Calif.)
Sensitive detection of viral nucleic acids is critically important for diagnosis and monitoring of the progression of infectious diseases such as those caused by SARS-CoV2, HIV-1, and other viruses. In HIV-1 infection cases, assessing the efficacy of treatment interventions that are superimposed on combination antiretroviral therapy (cART) has bene...
Telwatte, Sushama Martin, Holly Anne Marczak, Ryan Fozouni, Parinaz Vallejo-Gracia, Albert Kumar, G Renuka Murray, Victoria Lee, Sulggi Ott, Melanie Wong, Joseph K
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Published in
Methods (San Diego, Calif.)
The replication of SARS-CoV-2 and other coronaviruses depends on transcription of negative-sense RNA intermediates that serve as the templates for the synthesis of positive-sense genomic RNA (gRNA) and multiple different subgenomic mRNAs (sgRNAs) encompassing fragments arising from discontinuous transcription. Recent studies have aimed to character...
Piermatteo, Lorenzo Scutari, Rossana Chirichiello, Riccardo Alkhatib, Mohammad Malagnino, Vincenzo Bertoli, Ada Iapadre, Nerio Ciotti, Marco Sarmati, Loredana Andreoni, Massimo
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Published in
Methods (San Diego, Calif.)
Droplet digital PCR is an innovative and promising approach for highly sensitive quantification of nucleic acids that is being increasingly used in the field of clinical virology, including the setting of hepatitis B virus (HBV). Here, we comprehensively report a robust and reproducible ddPCR assay for the highly sensitive quantification of serum H...
