Dumbović, Gabrijela Sanjuan, Xavier Perucho, Manuel Forcales, Sonia-V
Published in
Methods (San Diego, Calif.)
Super resolution microscopy has changed our capability to visualize and understand spatial arrangements of RNA- and protein-containing domains in individual cells. In a previous study, we described a novel lncRNA, Tumor-associated NBL2 transcript (TNBL), which originates from a primate specific macrosatellite repeat. We aimed to describe several as...
Morselli, Marco Farrell, Colin Rubbi, Liudmilla Fehling, Heather L Henkhaus, Rebecca Pellegrini, Matteo
Published in
Methods (San Diego, Calif.)
Cytosine methylation is one of the best studied epigenetic modifications. In mammals, DNA methylation patterns vary among cells and is mainly found in the CpG context. DNA methylation is involved in important processes during development and differentiation and its dysregulation can lead to or is associated with diseases, such as cancer, loss-of-im...
Li, Yuanyuan
Published in
Methods (San Diego, Calif.)
The definition of epigenetics refers that molecular modifications on DNA that can regulate gene activity are independent of DNA sequence and mitotically stable. Notably, epigenetics studies have grown exponentially in the past few years. Recent progresses that lead to exciting discoveries and groundbreaking nature of this area demand thorough metho...
Rahman, Mohammad Mijanur Tollefsbol, Trygve O
Published in
Methods (San Diego, Calif.)
Cancer therapeutics is an ever-evolving field due to incessant demands for effective and precise treatment options. Over the last few decades, cancer treatment strategies have shifted somewhat from surgery to targeted precision medicine. CRISPR-dCas9 is an emerging version of precision cancer therapy that has been adapted from the prokaryotic CRISP...
Pajares, María J Palanca-Ballester, Cora Urtasun, Raquel Alemany-Cosme, Ester Lahoz, Agustin Sandoval, Juan
Published in
Methods (San Diego, Calif.)
Methylation of CpG dinucleotides plays a crucial role in the regulation of gene expression and therefore in the development of different pathologies. Aberrant methylation has been associated to the majority of the diseases, including cancer, neurodegenerative, cardiovascular and autoimmune disorders. Analysis of DNA methylation patterns is crucial ...
Nakato, Ryuichiro Sakata, Toyonori
Published in
Methods (San Diego, Calif.)
Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is a central method in epigenomic research. Genome-wide analysis of histone modifications, such as enhancer analysis and genome-wide chromatin state annotation, enables systematic analysis of how the epigenomic landscape contributes to cell identity, development, lineage specification,...
Kumar, Sumit Sharma, Prem Prakash Upadhyay, Charu Kempaiah, Prakasha Rathi, Brijesh Poonam,
Published in
Methods (San Diego, Calif.)
Novel coronavirus SARS-CoV-2 continues to spread rapidly worldwide and causing serious health and economic loss. In the absence of any effective treatment, various in-silico approaches are being explored towards the therapeutic discovery against COVID-19. Targeting multiple key enzymes of SARS-CoV-2 with a single potential drug could be an importan...
Ceciliani, F Ávila Morales, G De Matteis, G Grandoni, F Furioso Ferreira, R Roccabianca, P Lecchi, C
Published in
Methods (San Diego, Calif.)
Monocytes and macrophages belong to the mononuclear phagocyte system and play important roles in both physiological and pathological processes. The cells belonging to the monocyte/macrophage system are structurally and functionally heterogeneous. Several subsets of monocytes have been previously identified in mammalian blood, generating different s...
Girard, C L Graulet, B
Published in
Methods (San Diego, Calif.)
Clinical symptoms of B vitamin deficiency are rarely observed in ruminants because these vitamins are synthesized by the rumen microbiota. However, over the last decades, numerous reports of beneficial effects on production and metabolic efficiency of dairy cows have been published supporting that, under some conditions, B vitamin subclinical defic...
Andersen, Thea Os Kunath, Benoit J Hagen, Live H Arntzen, Magnus Ø Pope, Phillip B
Published in
Methods (San Diego, Calif.)
The rumen microbiome constitutes a dense and complex mixture of anaerobic bacteria, archaea, protozoa, virus and fungi. Collectively, rumen microbial populations interact closely in order to degrade and ferment complex plant material into nutrients for host metabolism, a process which also produces other by-products, such as methane gas. Our unders...
