Published in Journal of separation science
Accelerated technological progress and increased complexity of interrogated matrices imposes a demand for fast, powerful, and resolutive analysis techniques. Gas chromatography has been for a long time a 'go-to' technique for the analysis of mixtures of volatile and semi-volatile compounds. Coupling of the several dimensions of gas chromatography s...
Published in Journal of separation science
Substance P and hemokinin-1 were predominantly examined by immunoassays with their limitation to differentiate appropriately between both peptides. The use of liquid chromatography coupled with tandem mass spectrometry is a promising, highly selective alternative. Adsorption processes have been identified in preliminary experiments to play a crucia...
Published in Journal of separation science
Advances in the area of sample preparation are significant and have been growing significantly in recent years. This initial step of the analysis is essential and must be carried out properly, consisting of a complicated procedure with multiple stages. Consequently, it corresponds to a potential source of errors and will determine, at the end of th...
Published in Journal of separation science
The efficiency of a molecularly imprinted polymer as a selective packing material for the solid-phase extraction of imatinib mesylate sorption was investigated. The molecularly imprinted polymer was prepared using N,N'-methylenebisacrylamide as a cross-linker agent, N-vinylcaprolactam as a thermo-sensitive monomer, 1-vinyl-2-pyrrolidone and methyl ...
Published in Journal of separation science
To evaluate the xanthine oxidase inhibitory activity of the chemical constituents of Ligustrum lucidum in vitro, the spectrum-effect relationship was investigated. The high-performance liquid chromatography fingerprint was established by ultraviolet spectrophotometry, and the xanthine oxidase inhibitory activity was tested in vitro by a high-throug...
Published in Journal of separation science
A method of simultaneous separation and indirect ultraviolet detection of different valence iron ions Fe2+ and Fe3+ by using ionic liquids as mobile phase additives and ultraviolet absorption reagents on a cation exchange column functionalized with carboxylic acid group was developed. The effects of ionic liquids, organic acids, detection wavelengt...
Published in Journal of separation science
Microfluidic chip electrophoresis has been widely employed for separation of various biochemical species owing to its advantages of low sample consumption, low cost, fast analysis, high throughput, and integration capability. In this article, we reviewed the development of four different modes of microfluidics-based electrophoresis technologies inc...
Published in Journal of separation science
The interactive behavior of an amphipathic peptide with the Cu2+ , Ni2+ , and Zn2+ complexes of 1,4-bis(triazacyclonon-1-yl)butane), bis(tacn)but , immobilized onto Sepharose CL-4B, has been investigated. The effects of incubation time, as well as the incubation buffer pH and ionic strength, have been examined. The binding data have been interrogat...
Published in Journal of separation science
Two proposed syringe membrane filter solid phase microextraction and syringe membrane filter liquid/solid phase microextraction, coupled separately with high performance liquid chromatography, were developed for simultaneous enrichment and determination of the trace level of flavonoids in traditional Chinese medicine. In syringe membrane filter sol...
Published in Journal of separation science
Adopting a stationary phase convention circumvents problematic definition of the boundary between the stationary and the mobile phase in the liquid chromatography, resulting in thermodynamically consistent and reproducible chromatographic data. Three stationary phase definition conventions provide different retention data, but equal selectivity: (i...
