Corrigendum to "A high-performance liquid chromatography-tandem mass spectrometry method for the analysis of lifirafenib...
Published in Journal of pharmaceutical and biomedical analysis
Structural characterization and discrimination of Ophiopogon japonicas (Liliaceae) from different geographical origins b...
...Published in Journal of pharmaceutical and biomedical analysis
The root tuber of Ophiopogon japonicus (Thunb.) Ker-Gawl ("Maidong" in Chinese), with steroidal saponins and homoisoflavonoids as its representative chemical compositions, is a representative medicinal herbs with multiple major producing areas. This study aimed to distinguish the O. japonicas samples from Zhejiang and Sichuan by using an ultra-perf...
Coupling non-denaturing chromatography to mass spectrometry for the characterization of monoclonal antibodies and relate...
Published in Journal of pharmaceutical and biomedical analysis
The hyphenation of non-denaturing liquid chromatographic (LC) modes (ion exchange (IEX), size exclusion (SEC) and hydrophobic interaction chromatography (HIC)) with mass spectrometry (MS) has attracted significant attention in the last few years. The inherent problem of these couplings is that non-denaturing LC separations have tended to use non-vo...
Universal quantification method of degradation impurities in 16-membered macrolides using HPLC-CAD and study on source o...
Published in Journal of pharmaceutical and biomedical analysis
In terms of risk assessment especially for the impurities with different ultraviolet (UV) absorptions in 16-membered macrolides produced by fermentation, quantification without the availability of corresponding reference substances currently poses a challenge. In this study, a reliable method was established for the analysis of impurities in 16-mem...
Development of an ICP-MS/MS approach for absolute quantification and determination of phosphodiester to phosphorothioate...
Published in Journal of pharmaceutical and biomedical analysis
A new analytical method based on ICP-MS/MS is proposed for the characterization of synthetic phosphorothioate oligonucleotides. Absolute quantification of oligonucleotides is challenging, as well as the determination of phosphodiester to phosphorothioate ratio for phosphorothioate oligonucleotides. Both are considered as critical quality attributes...
Magnetic solid phase extraction followed with LC-MS/MS for determination of glimepiride in beagle dog plasma and its app...
Published in Journal of pharmaceutical and biomedical analysis
In this study, the core-shell polydopamine-coated magnetic nanomaterials were synthesized with one-step approach and applied as magnetic solid phase extraction adsorbents combined with LCMS/MS for quantification of the diabetes drug glimepiride in beagle dog plasma. The [email protected] nanomaterials have strong magnetic response, good dispersibility in a...
UPLC-MS identification and anticomplement activity of the metabolites of Sophora tonkinensis flavonoids treated with hum...
Published in Journal of pharmaceutical and biomedical analysis
Anticomplement activity played an important role in anti-inflammatory effects of traditional Chinese herbs. The total flavonoids of Sophora tonkinensis (TFST) were inactive on the complement system but showed obvious anticomplement activity after incubated with human intestinal bacteria in vitro. In order to discover the metabolic activation of TFS...
Quantitation of serum pregnanediol-3-glucuronide level in different endocrine statuses by LC-MS/MS.
...Published in Journal of pharmaceutical and biomedical analysis
Pregnanediol-3-glucuronide (PdG) is the major terminal metabolite of progesterone, playing an important role in physiological processes, such as the female menstrual cycle, pregnancy (supports gestation), embryogenesis and maternal immune response of humans and other species. Hence, accurate measurement of PdG in serum/plasma is needed for the eval...
Conventional and nonconventional extraction techniques for optimal extraction processes of rosmarinic acid from six Lami...
Published in Journal of pharmaceutical and biomedical analysis
The goal of this study was to improve the extraction efficiency of rosmarinic acid (RA) from Lamiaceae herbs (lemon balm, peppermint, oregano, rosemary, sage, and thyme) using various extraction techniques (maceration with stirring, MACS; heat reflux, HRE; and microwave-assisted extraction, MAE) and extraction conditions (solvent acidity, solvent t...
Mass spectrometry-based methods in characterization of the higher order structure of protein therapeutics.
Published in Journal of pharmaceutical and biomedical analysis
Higher order structure of protein therapeutics is an important quality attribute, which dictates both potency and safety. While modern experimental biophysics offers an impressive arsenal of state-of-the-art tools that can be used for the characterization of higher order structure, many of them are poorly suited for the characterization of biopharm...
