Senescent bone marrow microenvironment promotes Nras-mutant leukemia.
Published in Journal of molecular cell biology
QKI-5 regulates the alternative splicing of cytoskeletal gene ADD3 in lung cancer.
...Published in Journal of molecular cell biology
Accumulating evidence indicates that the alternative splicing program undergoes extensive changes during cancer development and progression. The RNA-binding protein QKI-5 is frequently down-regulated and exhibits anti-tumor activity in lung cancer. However, little is known about the functional targets and regulatory mechanism of QKI-5. Here, we rep...
Efficient replacement of long DNA fragments via non-homologous end joining at non-coding regions.
...Published in Journal of molecular cell biology
Genomic DNA replacement for achieving sophisticated genetic manipulation is implemented currently through homogenous recombination/homology-dependent repair (HR/HDR). Here we report a novel DNA fragment replacement method that is mediated by non-homologous end joining (NHEJ)-dependent DNA repair at two sites of CRISPR/Cas9-induced double-strand bre...
A novel isoform of ATOH8 promotes the metastasis of breast cancer by regulating RhoC.
...Published in Journal of molecular cell biology
Metastases are the main cause of cancer-related mortality in breast cancer. Although significant progress has been made in the field of tumor metastasis, the exact molecular mechanisms involved in tumor metastasis are still unclear. Here, we report that ATOH8-V1, a novel isoform of ATOH8, is highly expressed in breast cancer and is a negative progn...
LncRNA Platr22 promotes super-enhancer activity and stem cell pluripotency.
Published in Journal of molecular cell biology
Super-enhancers (SEs) comprise large clusters of enhancers, which are co-occupied by multiple lineage-specific and master transcription factors, and play pivotal roles in regulating gene expression and cell fate determination. However, it is still largely unknown whether and how SEs are regulated by the non-coding portion of the genome. Here, throu...
A 'Goldmine' for digging cancer-specific targets: the genes essential for embryo development but non-essential for adult...
Published in Journal of molecular cell biology
Inhibition of tumor suppressor p73 by nerve growth factor receptor via chaperone-mediated autophagy.
Published in Journal of molecular cell biology
The tumor suppressr p73 is a homolog of p53 and is capable of inducing cell cycle arrest and apoptosis. Here, we identify nerve growth factor receptor (NGFR, p75NTR, or CD271) as a novel negative p73 regulator. p73 activates NGFR transcription, which, in turn, promotes p73 degradation in a negative feedback loop. NGFR directly binds to p73 central ...
Abl1 deletion in gut stem cells suppresses p53 induction and promotes colitis-associated tumor formation.
Published in Journal of molecular cell biology
MDMX phosphorylation-dependent p53 downregulation contributes to an immunosuppressive tumor microenvironment.
Published in Journal of molecular cell biology
A role of tumor-suppressive activity of p53 in the tumor microenvironment (TME) has been implicated but remains fairly understudied. To address this knowledge gap, we leveraged our MdmxS314A mice as recipients to investigate how implanted tumor cells incapacitate host p53 creating a conducive TME for tumor progression. We found that tumor cell-asso...
Cancer-associated adipocyte-derived G-CSF promotes breast cancer malignancy via Stat3 signaling.
...Published in Journal of molecular cell biology
Adipocyte is the most predominant cell type in the tumor microenvironment of breast cancer and plays a pivotal role in cancer progression, yet the underlying mechanisms and functional mediators remain elusive. We isolated primary preadipocytes from mammary fat pads of human breast cancer patients and generated mature adipocytes and cancer-associate...
