Glässner, Andreas Wurpts, Gerda Röseler, Stefanie Yazdi, Amir S Krämer, Christine Fatangare, Amol Sickmann, Albert Hoffmann, Per Nöthen, Markus Sachs, Bernhardt
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Published in
Journal of immunological methods
The lymphocyte transformation test (LTT) is used for the in vitro detection of a drug sensitization in assumed drug allergic patients. It is based on the detection of antigen (drug)-specific activation of T cells indicated by e.g. proliferation or cytokine secretion. However, occasional stimulatory effects of the drug unrelated to specific drug-all...
Linggi, Bryan Cremer, Jonathan Wang, Zhongya Van Viegen, Tanja Vermeire, Séverine Lefevre, Pavine Shackelton, Lisa M Jairath, Vipul Teft, Wendy Vande Casteele, Niels
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Published in
Journal of immunological methods
Clinical trials of novel therapies for the treatment of ulcerative colitis (UC) may benefit from immune cell profiling, however implementation of this methodology is limited in the multicenter trial setting by necessity of timely (within 6 to 8 h) isolation and processing of peripheral blood mononuclear cells (PBMC) from whole blood samples. Becton...
Fernandez, Zoraida de Arruda Rodrigues, Rudielle Torres, Jaire Marinho Marcon, Gláucia Elisete Barbosa de Castro Ferreira, Eduardo de Souza, Vanessa Felipe Sarti, Elaine Fernandes Baez Bertolli, Guilherme Ferminao Araujo, Daniel Demarchi, Luiz Henrique Ferraz
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Published in
Journal of immunological methods
Serological tests developed for COVID-19 diagnostic are based on antibodies specific for SARS-CoV-2 antigens. Most of the antigens consist of a fragment or a whole amino acid sequence of the nucleocapsid or spike proteins. We evaluated a chimeric recombinant protein as an antigen in an ELISA test, using the most conserved and hydrophilic portions o...
Zetlen, Hilary L Cao, Kevin T Schichlein, Kevin D Knight, Noelle Maecker, Holden T Nadeau, Kari C Rebuli, Meghan E Rice, Mary B
Published in
Journal of immunological methods
Multiplexed protein analysis platforms are a novel and efficient way to characterize biomarkers in a variety of biological samples. Few studies have compared protein quantitation and reproducibility of results across platforms. We utilize a novel nasosorption technique to collect nasal epithelial lining fluid (NELF) from healthy subjects, and compa...
Samsel, Jakob Boswell, Kristin L Watkins, Timothy Ambrozak, David R Mason, Rosemarie Yamamoto, Takuya Ko, Sungyoul Yang, Yongping Zhou, Tongqing Doria-Rose, Nicole A
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Published in
Journal of immunological methods
Many HIV-1 vaccines are designed to elicit neutralizing antibodies, and pre-clinical testing is often carried out in rhesus macaques (RMs). We have therefore adapted a method of B cell immortalization for use with RM B cells. In this system, RM B cells are activated with CD40 ligand and RM IL-21 before transduction with a retroviral vector encoding...
Chatterjee, Dipankor Al Rimon, Razoan Chowdhury, Umar Faruq Islam, Mohammad Riazul
Published in
Journal of immunological methods
Entamoeba histolytica, an anaerobic parasite, infects humans and other primates and causes fatal diseases, such as amebiasis, amebic liver abscesses, and many others. Thousands of people are infected and dying due to the need for a proper protective cure, especially in poor sanitizing regions, such as Latin America, Asia, and Africa. Around 10% of ...
Varela, Vanessa Araújo da Silva Heinen, Letícia Borges Marti, Luciana Cavalheiro Caraciolo, Victória Bulcão Datoguia, Tarcila Santos Amano, Mariane Tami Pereira, Welbert Oliveira
Published in
Journal of immunological methods
Myeloid-derived suppressor cells (MDSCs) are a heterogeneous population with a potent suppressor profile that regulates immune responses. These cells are one of the main components of the microenvironment of several diseases, including solid and hematologic tumors, autoimmunities, and chronic inflammation. However, their wide use in studies is limi...
Wu, Xuesong Clarke, William R Koplinski, Chad A Peterson, Francis C Dwinell, Michael B Wei, Grace Chao, Ellen Huynh, Mindy Yamada, Daisuke Volkman, Brian F
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Published in
Journal of immunological methods
A novel engineered CCL20 locked dimer (CCL20LD) is nearly identical to the naturally occurring chemokine CCL20 but blocks CCR6-mediated chemotaxis and offers a new approach to treat the diseases of psoriasis and psoriatic arthritis. Methods for quantifying CCL20LD serum levels are needed to assess pharmacokinetics parameters and evaluate drug deliv...
Alobaid, Meshal A
Published in
Journal of immunological methods
The utilization of T cells is becoming increasingly prominent in both clinical and research domains. However, the need to optimize preservation methodologies for extended periods of time remains unmet. To address this issue, we have developed a protocol for the handling and preservation of T cells that facilitates successful donor homologous co-cul...
Schwab, Lisa Bühler, Sarah Biedritzky, Anna Schmidt, Marina Andre, Maya C
Published in
Journal of immunological methods
Recent studies indicate that under certain conditions such as viral infection or exposure to pro-inflammatory cytokines, NK cells may acquire features of adaptive immune cells. In this context, various forms of adaptive NK cells have been described, i.e. "liver-resident" memory-like NK cells, cytomegalovirus (CMV)-induced memory NK cells and interl...
