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Postmortem survey of haemoglobin A1c, non-alcoholic steatohepatitis and liver fibrosis within a general population.

Published in Journal of clinical pathology

Non-alcoholic steatohepatitis (NASH), fatty liver disease and fibrosis are associated with diabetes mellitus and obesity. Previous autopsy series have reported prevalence of fatty liver disease to be 11%-24%. Recent studies, using imaging and serology, suggest a prevalence of 20%-35%, NASH of 5% and advanced fibrosis of 2%-3%. We examined the preva...

Implementation of the updated NICE haematological cancers (NG47) improving outcomes guidelines across Specialist Integra...

Published in Journal of clinical pathology

Haematological malignancies represent a diverse group of diseases with complex diagnostic requirements. National Institute for Health and Care Excellence (NICE) Haematological Cancer: Improving Outcomes Guidance was published in 2003 and updated in 2016 (NG47), providing recommendations for service delivery including Specialist Integrated Haematolo...

Beyond the routine CBC: machine learning and statistical analyses identify research CBC parameter associations with myel...

Published in Journal of clinical pathology

Given the time, expense and clinical expertise required for a myelodysplastic syndrome (MDS) diagnosis, there is a clear need for a cost-effective screening laboratory test that can rapidly and accurately distinguish patients with cytopenias related to MDS from other causes. We measured conventional and research use only complete blood cell (CBC) p...

Quantitative p53 immunostaining aids in the detection of prevalent dysplasia.

Published in Journal of clinical pathology

The lack of accepted scoring criteria has precluded the use of p53 in routine practice. We evaluate the utility of automated quantitative p53 analysis in risk stratifying Barrett's oesophagus (BE) patients using non-dysplastic BE (NDBE) biopsies in a multicentric cohort of BE progressor (P) and non-progressor (NP) patients. NDBE biopsies prior to t...

Improving care of melanoma patients through efficient, integrated cellular-molecular pathology workflows using tissue sa...

Published in Journal of clinical pathology

The aim of this quality improvement project was to improve the turnaround time of B-raf proto-oncogene (BRAF) mutation testing in patients with malignant melanoma to support oncologists in making timely treatment decisions. This is a prospective in-house verification of the Idylla BRAF test as compared with DNA panel next-generation sequencing (NGS...

MYCN amplification and International Neuroblastoma Risk Group stratification on fine-needle aspiration biopsy and their ...

Published in Journal of clinical pathology

Risk stratification as per the International Neuroblastoma Risk Group (INRG) stratification is important for management of neuroblastoma. INRG incorporates various parameters including histological category as per the International Neuroblastoma Pathology Classification (INPC) and MYCN amplification, which were evaluated in fine needle aspiration b...

Ketamine: friend or foe?

Published in Journal of clinical pathology

One-step genotyping of α-thalassaemia by multiplex symmetric PCR melting curve.

Published in Journal of clinical pathology

Alpha-thalassaemia is one of the most common monogenic disorders worldwide. Due to high guanine-cytosine (GC) content and high mutation diversity in α-globin gene cluster, deletional and non-deletional mutations were usually separately detected with different methods. The aim of this study was to develop a novel one-step method for α-thalassaemia g...

Failure to thrive in a man in his late forties.

Published in Journal of clinical pathology

Development of a LAG-3 immunohistochemistry assay for melanoma.

Published in Journal of clinical pathology

A robust immunohistochemistry (IHC) assay was developed to detect lymphocyte-activation gene 3 (LAG-3) expression by immune cells (ICs) in tumour tissues. LAG-3 is an immuno-oncology target with demonstrable clinical benefit, and there is a need for a standardised, well-characterised assay to measure its expression. This study aims to describe LAG-...