Xiong, Hai Huang, Te-Yang Chang, Yu-Lo Su, Wen-Ta
Published in
Journal of bioscience and bioengineering
Osteoarthritis, the most common joint disease worldwide, is a degenerative disease characterized by cartilage degeneration and inflammation. The active ingredients in the traditional Chinese medicinal plant Achyranthes bidentate can be used to treat waist, leg, and joint pain caused by rheumatism arthralgia. In this study, we identified the optimal...
Moriyama, Kousuke Inomoto, Noe Moriuchi, Hidetoshi Nihei, Masanobu Sato, Miku Miyagi, Yoshiki Tajiri, Ayaka Sato, Takeshi Tanaka, Yasuhiko Johno, Yuuki
...
Published in
Journal of bioscience and bioengineering
Albumin is an attractive component for the development of biomaterials applied as biomedical implants, including drug carriers and tissue engineering scaffolds, because of its high biocompatibility and low immunogenicity. Additionally, albumin-based gelators facilitate cross-linking reactions under mild conditions, which maintains the high viabilit...
Nishitani, Atsushi Hiramatsu, Kentaro Kadooka, Chihiro Mori, Kazuki Okutsu, Kayu Yoshizaki, Yumiko Takamine, Kazunori Tashiro, Kosuke Goto, Masatoshi Tamaki, Hisanori
...
Published in
Journal of bioscience and bioengineering
A putative methyltransferase, LaeA, controls citric acid production through epigenetic regulation of the citrate exporter gene, cexA, in the white koji fungus Aspergillus luchuensis mut. kawachii. In this study, we investigated the role of another epigenetic regulator, heterochromatin protein 1, HepA, in citric acid production. The ΔhepA strain exh...
Mei, Jiajia Liu, Xiaojie Liu, Xinyi Bao, Yihong Luo, Ting Wang, Jinling
Published in
Journal of bioscience and bioengineering
In current research, yeast species Issatchenkia terricola WJL-G4 was shown to be capable of degrading citric acid, especially in the processing of fruit juice and wine. I. terricola WJL-G4 was able to use citric acid as a carbon source, but the metabolic effects of citric acid on yeast remained unclear. In this study, the metabolic effects of citri...
Cao, Wei Watanabe, Risa Ishii, Yoshitaka Kirimura, Kohtaro
Published in
Journal of bioscience and bioengineering
Several alkyl glucosides exhibit various bioactivities. 1-Octyl β-d-glucopyranoside produced by organic synthesis is used as a nonionic surfactant. However, no convenient method has been developed for the selective production of alkyl α-glucosides (α-AGs), such as 1-octyl α-d-glucopyranoside (α-OG). Therefore, we developed a simple method for selec...
Kaneko, Masahiro Moriguchi, Hiroaki Futatsubashi, Ryo Ayano, Satoru Kobayashi, Goro Ito, Akira
Published in
Journal of bioscience and bioengineering
Cell-based therapy is an attractive approach, and encapsulation of therapeutic cells is a promising strategy because it prevents immune responses and allows transplanted cells to be retrieved in case of dysfunction. Bioartificial pancreas, in which insulin-secreting cells are encapsulated in a semipermeable membrane bag, is a new class of medical d...
Kojima, Kenji Morimoto, Kenta Juma, Kevin Maafu Takita, Teisuke Saito, Kazuki Yanagihara, Itaru Fujiwara, Shinsuke Yasukawa, Kiyoshi
Published in
Journal of bioscience and bioengineering
Recombinase polymerase amplification (RPA) is an isothermal DNA amplification reaction at around 41°C using recombinase (Rec), single-stranded DNA-binding protein (SSB), strand-displacing DNA polymerase (Pol), and an ATP-regenerating enzyme. In this study, we attempted to use pyruvate kinase instead of creatine kinase (CK) that has been consistentl...
Kirimoto, Yutaka Yamano-Adachi, Noriko Koga, Yuichi Omasa, Takeshi
Published in
Journal of bioscience and bioengineering
Therapeutic antibodies are attractive biopharmaceuticals because of their high therapeutic effects, fewer side effects, and prolonged half-life in the blood. Chinese hamster ovary (CHO) cells are the most widely used host cell lines to produce therapeutic antibodies in industries. High-producing recombinant CHO cells can be established via overexpr...
Kaneko, Masahiro Sato, Airi Ayano, Satoru Fujita, Akio Kobayashi, Goro Ito, Akira
Published in
Journal of bioscience and bioengineering
Microcarriers provide a high surface-area-to-volume ratio that can realize high yields of cell products, including human mesenchymal stem cells (hMSCs). Here, we report a novel poly(vinyl alcohol) (PVA)-based microcarrier for hMSC expansion in suspension culture. PVA microcarriers were prepared as collagen-coated PVA hydrogels 181 μm in size and a ...
Mao, Fengjiao Huang, Jun Zhou, Rongqing Qin, Hui Zhang, Suyi Cai, Xiaobo Qiu, Chuanfeng
Published in
Journal of bioscience and bioengineering
Complex microbiomes of pit mud play significant roles in imbuing flavors and qualities of Nongxiang Baijiu during fermentation. However, pit mud microbial enrichment and succession is a long process that is also accompanied by aging. Development of high-quality artificial pit mud becomes an urgent problem. In this study, a new medium based on space...
