Yang, Jie Lu, Jing Zhu, Qingzheng Tao, Yang Zhu, Qiang Guo, Changming Fang, Yaowei Chen, Li Koyande, Apurav Krishna Wang, Shujun
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Published in
Journal of bioscience and bioengineering
As one of Lianyungang's most famous specialties, Acanthogobius hasta is delicious and nutritious fish, but is extremely susceptible to spoilage during transportation and storage. In this study, Lactobacillus plantarum MMB-07 was isolated from traditional fermented sour fish to reduce losses and improve the utilization and food value of A. hasta. L....
Poornima, Shanmugam Divya, Palanisamy Karmegam, Natchimuthu Karthik, Vivekanandhan Subbaiya, Ramasamy
Published in
Journal of bioscience and bioengineering
Microbial production of xylanase is gaining the commercial importance, due to its wide range of applications from paper and pulp to food and feed industries. Streptomyces geysiriensis was used for the production of extracellular xylanase from lignocellulosic substrates such as rice bran and saw dust, under solid-state fermentation. The influence of...
Doi, Shiori Komatsu, Mamoru Ikeda, Haruo
Published in
Journal of bioscience and bioengineering
To improve the production of secondary metabolites by alternation of the carbon metabolic flux, two types of deletion mutants of the central metabolic pathway, the Embden-Meyerhof (EM) or pentose phosphate (PP) pathway, in the genetically engineered Streptomyces avermitilis were constructed. Double-deletion mutants of phosphofructokinase (ΔpfkA1Δpf...
Ye, Bin Luo, Yanfei Zhong, Bangchao Zhu, Jianchun Huang, Junwei Gu, Jiayu Jiang, Jiandong Yan, Xin He, Jian He, Qin
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Published in
Journal of bioscience and bioengineering
To achieve the high-level stable expression of chlorothalonil hydrolytic dehalogenase (Chd), the gene chd was first integrated into the chromosome of Bacillus subtilis WB800. High generation stability was achieved by almost no gene lost after six generations but Chd activity decreased. aprE promoter alteration, translation initiation region modific...
Yamamoto, Chiaki Toyoshima, Masakazu Kitamura, Sayaka Ueno, Yoshifumi Akimoto, Seiji Toya, Yoshihiro Shimizu, Hiroshi
Published in
Journal of bioscience and bioengineering
Photosynthetic organisms produce ATP and NADPH using light as an energy source and further utilize these cofactors during metabolism. Photosynthesis involves linear and cyclic electron flows; as the cyclic electron flow produces ATP more effectively than the linear electron flow without NADPH, the cell efficiently adjusts ATP and NADPH production u...
Chen, Lurui Du, Shiyun Xie, Li
Published in
Journal of bioscience and bioengineering
Ex-situ biogas upgrading based on hydrogenotrophic methanogenic process has attracted much attention with the depletion of fossil fuels. Consumption of CO2 leads to the pH increase in the mixed cultures of biogas upgrading system. The hydrogenotrophic methanogens were enriched at pH 5.5-6.0, 7.0-7.5, and 8.5-9.0 and at 55°C and 70°C. The methane pr...
Kitamura, Sayaka Shimizu, Hiroshi Toya, Yoshihiro
Published in
Journal of bioscience and bioengineering
Identification of the rate-limiting step in a metabolic pathway is an important challenge in metabolic engineering for enhancing pathway flow. Although specific enzyme activities (Vmax) provide valuable clues for the identification, it is time-consuming and difficult to measure multiple enzymes in the pathway because different assay protocols are r...
Matsudaira, Akiko Hoshino, Yasushi Uesaka, Kazuma Takatani, Nobuyuki Omata, Tatsuo Usuda, Yoshihiro
Published in
Journal of bioscience and bioengineering
Cyanobacteria can grow photoautotrophically, producing a range of substances by absorbing sunlight and utilizing carbon dioxide, and can potentially be used as industrial microbes that have minimal sugar requirements. To evaluate this potential, we explored the possibility of l-glutamate production using the Synechocystis sp. PCC6803. The ybjL gene...
Jing, Chang-Qin Guo, Meng-Long Wang, Chong Ni, Tian-Jun Guo, Xiao Wang, Tian-Yun
Published in
Journal of bioscience and bioengineering
Like endogenous proteins, recombinant foreign proteins produced in human cell lines also need post-translational modifications. However, high and long-term expression of a gene of interest (GOI) presents significant challenges for recombinant protein production in human cells. In this work, the effect of human matrix attachment region elements (MAR...
Yoshida, Chihiro Murakami, Miyabi Niwa, Anna Takeya, Masahiro Osanai, Takashi
Published in
Journal of bioscience and bioengineering
C-Phycocyanin (PC) is a protein used commercially as a natural blue pigment produced by cyanobacteria, cryptophytes, and rhodophytes. Although it is industrially synthesized from the cyanobacterium Arthrospira platensis, PC requires high levels of energy for its extraction, which involves freezing of cells. However, as a protein, PC is easily denat...
