Maulana, Aulia Adiandri, Resa Setia Boopathy, Ramaraj Setiadi, Tjandra
Published in
Journal of bioscience and bioengineering
Textile wastewater treatment generates sludge that needs to be disposed of safely. The cost of sludge management is 50% of the total treatment cost of the wastewater. To reduce the expense, the sludge can be repurposed as a valuable resource by extracting extracellular polymeric substance (EPS). EPS contains polysaccharides, proteins, and humic sub...
Yuan, Lin Qin, Yan-Li Zou, Zhi-Cheng Appiah, Bright Huang, Hao Yang, Zhong-Hua Qun, Can
Published in
Journal of bioscience and bioengineering
The intracellular NAD(P)H insufficiency is the key factor which limits the reduced product (such as chiral alcohols) synthesis by whole cell biocatalysis or microbial cell factory. In this paper, we reported a novel solution to increase NADPH supply through strengthening the pentose phosphate pathway (PPP) flux with overexpression of extra zwf (gen...
Kageyama, Tatsuto Anakama, Riki Togashi, Hideru Fukuda, Junji
Published in
Journal of bioscience and bioengineering
Hair follicle morphogenesis is triggered by epithelial-mesenchymal interactions. Several approaches have been developed for preparing hair follicle organoids using epithelial and mesenchymal cells; however, the current understanding of the relevance of in vitro spontaneous organization processes to hair regeneration is limited. In the present study...
Taokaew, Siriporn Nakson, Nawachon Thienchaimongkol, Jirath Kobayashi, Takaomi
Published in
Journal of bioscience and bioengineering
In Gluconacetobacter xylinus cultivation for bacterial nanocellulose production, agro-industrial wastes, soybean residual okara, okara extracted protein, and modified okara protein, were used as a protein source. In comparison with homogenized raw okara and protein extracted from raw okara, acetic-acid modified protein provided the higher cellulose...
Çam Derin, Dilek Gültekin, Enes İçen Taşkın, Irmak Yakupoğulları, Yusuf
Published in
Journal of bioscience and bioengineering
SARS-CoV-2 is still threat for humanity and its detection is crucial. Although real time reverse transcriptase polymerase chain reaction is the most reliable method for detection of N protein genes, alternative methods for molecular detection are still needed. Thus, lateral flow assay models for 2019-nCoV_ N3 were developed for molecular detection....
Suzuki, Hiromitsu Mori, Reini Kato, Masashi Shimizu, Motoyuki
Published in
Journal of bioscience and bioengineering
The white-rot fungus Phanerochaete chrysosporium can degrade lignin polymers using extracellular, non-specific, one-electron oxidizing enzymes. This results in the formation of guaiacyl (G), syringyl (S), and hydroxyphenyl (H) units, such as vanillic acid, syringic acid, and p-hydroxybenzoic acid (p-HBA) and the corresponding aldehydes, which are f...
Yamamoto, Yotaro Kajiura, Hiroyuki Nishibu, Takahiro Fujiyama, Kazuhito
Published in
Journal of bioscience and bioengineering
The production of recombinant proteins using insect cells has been widely used for over 30 years, which contributing to life science research and biotechnology. Insect cells exhibiting enhanced N-glycosylation and recombinant protein productivity enhance the productivity of the baculovirus-insect cell system (BICS). A new highly proliferative insec...
Meng, Guoliang Ye, Liyun Ma, Shuili Wu, Longyue Deng, Youjin Wu, Xiaoping
Published in
Journal of bioscience and bioengineering
Several mitogenomes of the genus Ganoderma have been assembled, but intraspecific comparisons of mitogenomes in Ganoderma lingzhi have not been reported. In this study, 19 G. lingzhi mitogenomes were assembled and analyzed combined with three mitogenomes of G. lingzhi from GenBank in term of the characteristics, evolution, and phylogeny. The result...
Dong, Dejin Wang, Xinyi Zong, Hong Lu, Xinyao Zhuge, Bin
Published in
Journal of bioscience and bioengineering
Due to the lack of available episomal plasmid, the improvement of many industrial strains, especially exogenous gene expression, is severely restricted. The failure of autonomous replication or low copy number of episomal plasmids is the main reason for the failure of many episomal plasmids construction. In this paper, Candida glycerinogenes, an in...
Sugiura, Shinji Shin, Kazumi Kanamori, Toshiyuki
Published in
Journal of bioscience and bioengineering
This paper reports perfusion culture of human umbilical vein endothelial cells (HUVECs) on a microporous membrane in a pressure-driven microphysiological system (PD-MPS), which we developed previously as a multi-throughput perfusion culture platform. We designed fluidic culture unit with microporous membrane to culture HUVECs under fluidic shear st...
