Molecular and Functional Diversity of The Oxytocinase Subfamily of M1 Aminopeptidases.
Published in Journal of biochemistry
circGFRA1 affects the sensitivity of triple negative breast cancer cells to paclitaxel via the miR-361-5p/TLR4 pathway.
Published in Journal of biochemistry
In recent years, the role of circular RNAs (circRNAs) in tumors has attracted widespread attention. Some circRNAs have been reported to play a role in triple negative breast cancer (TNBC), however, circRNAs have rarely been reported in terms of TNBC resistance. This study aimed to clarify that circGFRA1 affects the sensitivity of TNBC cells to pacl...
Silent information regulator type-1 mediates amelioration of inflammatory response and oxidative stress in lipopolysacch...
Published in Journal of biochemistry
Silent information regulator type-1 (SIRT1) is crucial during the development of acute respiratory distress syndrome (ARDS). We aimed to explore whether SIRT1 activation could protect against ARDS. SIRT1 was activated by its agonist SRT1720. ARDS was induced by intraperitoneal injection of 5 mg/kg lipopolysaccharide (LPS). Lung injuries were determ...
Long non-coding RNA DANCR accelerates colorectal cancer progression via regulating the miR-185-5p/HMGA2 axis.
Published in Journal of biochemistry
Long non-coding RNAs (lncRNAs) are crucial players in tumor progression. Herein, this work was designated to decipher the clinical significance, function and molecular mechanism of an lncRNA, differentiation antagonizing non-coding RNA (DANCR) in colorectal cancer (CRC). Quantitative real-time PCR (qRT-PCR) was adopted to examine DANCR, miR-185-5p ...
Escherichia coli amino acid auxotrophic expression host strains for investigating protein structure-function relationshi...
...Published in Journal of biochemistry
A set of C43(DE3) and BL21(DE3) Escherichia coli host strains that are auxotrophic for various amino acids is briefly reviewed. These strains require the addition of a defined set of one or more amino acids in the growth medium, and have been specifically designed for overproduction of membrane or water-soluble proteins selectively labeled with sta...
Emerging roles of extracellular vesicles in physiology and disease.
Published in Journal of biochemistry
Extracellular vesicles (EVs), such as exosomes and microvesicles, are small membrane vesicles secreted by almost all cell types and are abundant in blood; body fluids such as urine, spinal fluid, tears, and saliva; and cell culture media. From an evolutionary perspective, they are biologically significant as a means for expelling unwanted cellular ...
Pathogenic and protective roles of extracellular vesicles in neurodegenerative diseases.
Published in Journal of biochemistry
Neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis and polyglutamine diseases are caused by aggregation and abnormal accumulation of the disease-causative proteins in brain and spinal cord. Recent studies have suggested that proteins associated with neurodegenerative diseases are secreted and ...
Involvement of GcvB small RNA in intrinsic resistance to multiple aminoglycoside antibiotics in Escherichia coli.
Published in Journal of biochemistry
Deleting the gene for small RNA GcvB in Esherichia coli was found to increase the sensitivity to several aminoglycoside antibiotics, such as neomycin, streptomycin, kanamycin, kasugamycin and spectinomycin, at low concentrations. GcvB, conserved in gram-negative enteric bacteria, is known to negatively control the expression of many genes for amino...
Visualization of endogenous nuclear F-actin in mouse embryos reveals abnormal actin assembly after somatic cell nuclear ...
Published in Journal of biochemistry
Actin in the nucleus, referred to as nuclear actin, is involved in a variety of nuclear events. Nuclear actin is present as a globular (G-actin) and filamentous form (F-actin), and dynamic assembly/disassembly of nuclear actin profoundly affects nuclear functions. However, it is still challenging to observe endogenous nuclear F-actin. Here, we pres...
G-quadruplex binding protein Rif1, a key regulator of replication timing.
Published in Journal of biochemistry
DNA replication is spatially and temporally regulated during S phase to execute efficient and coordinated duplication of entire genome. Various epigenomic mechanisms operate to regulate the timing and locations of replication. Among them, Rif1 plays a major role to shape the "replication domains" that dictate which segments of the genome are replic...
