Published in
Journal of Virological Methods
Abstract An oligonucleotide ligation assay (OLA) designed to detect Human Immunodeficiency Virus type-1 (HIV)-drug-resistance to the nevirapine (NVP) selected mutations K103N, Y181C, V106M and G190A was used to evaluate 200 archived dried blood spots (DBS) from infected infants participating in the Zimbabwean Early Infant Diagnosis (EID) Program. C...
Zhang, Qingli Standish, Isaac Winters, Andrew D Puzach, Corey Ulferts, Rachel Ziebuhr, John Faisal, Mohamed
Published in
Journal of virological methods
Fathead minnow nidovirus (FHMNV) is a serious baitfish-pathogenic virus in North America. Studies to trace the spread of the virus and determine its host range are hampered by the absence of reliable diagnostic assays. In this study, a one-step, reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed that targets ...
Kania, Dramane Ottomani, Laure Meda, Nicolas Peries, Marianne Dujols, Pierre Bolloré, Karine Rénier, Wendy Viljoen, Johannes Ducos, Jacques Van de Perre, Philippe
...
Published in
Journal of virological methods
In-house developed real-time PCR (qPCR) techniques could be useful conjunctives to the management of hepatitis B virus (HBV) infection in resource-limited settings with high prevalence. Two qPCR assays (qPCR1 and qPCR2), based on primers/probes targeting conserved regions of the X and S genes of HBV respectively, were evaluated using clinical sampl...
Chang, Chia-Yi Deng, Ming-Chung Wang, Fun-In Tsai, Hsiang-Jung Yang, Chia-Huei Chang, Chieh Huang, Yu-Liang
Published in
Journal of virological methods
The porcine respiratory disease complex (PRDC) is the most common disease in commercial pork production worldwide. Porcine circovirus type 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV), the most important agents of PRDC, usually co-infect in the same pigs. In order to survey the prevalence of PCV2 and PRRSV in pigs of var...
Segawa, Takao Kobayashi, Yuki Sase, Yukina Itou, Takuya Suzuki, Miwa Endoh, Tomoko Nakanishi, Teruyuki Sakai, Takeo
Published in
Journal of virological methods
The development of rapid and simple gene amplification tests is required for detection of pathogens to prevent transmission of infectious diseases between animals or from animals to humans. An easy-to-use rapid gene amplification method that can directly detect RNA and DNA viruses in clinical samples was developed. This method is based on combining...
Liang, Xiao Chigerwe, Munashe Hietala, Sharon K Crossley, Beate M
Published in
Journal of virological methods
In order to improve the analytic quality of respiratory specimens collected from cattle for nucleic acid-based diagnosis, a study was undertaken to verify realtime PCR efficiency of specimens collected and stabilized on FTA Cards™, filter paper which is treated chemically. Nucleic acids collected using FTA Cards without the need for a cold-chain or...
Kandeil, Ahmed Bagato, Ola Zaraket, Hassan Debeauchamp, Jennifer Krauss, Scott El-Shesheny, Rabeh Webby, Richard J Ali, Mohamed A Kayali, Ghazi
Published in
Journal of virological methods
Low pathogenic influenza viruses grow readily in embryonated chicken eggs but require the addition of exogenous proteases to grow in MDCK cell culture. In this study, we found that the influenza viruses propagated previously in eggs, can grow for up to two passages in cell culture without the addition of exogenous proteolytic enzymes. These results...
de Vries, Rory D Ludlow, Martin Verburgh, R Joyce van Amerongen, Geert Yüksel, Selma Nguyen, D Tien McQuaid, Stephen Osterhaus, Albert D M E Duprex, W Paul de Swart, Rik L
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Published in
Journal of virology
Throughout history viral zoonoses have had a substantial impact on human health. Given the drive toward global eradication of measles, it is essential to understand the zoonotic potential of animal morbilliviruses. Morbilliviruses are thought to have evolved from a common ancestral virus that jumped species and adapted to new hosts. Recently, canin...
Hue, Erika S Fortier, Guillaume D Fortier, Christine I Leon, Albertine M Richard, Eric A Legrand, Loïc J Pronost, Stéphane L
Published in
Journal of virological methods
Equid gammaherpesviruses-2 and -5 are involved in respiratory problems, with potential clinical manifestations such as nasal discharge, pharyngitis and swollen lymph nodes. These viruses are sometimes associated with a poor-performance syndrome, which may result in a significant and negative economic impact for the horse industry. The aim of the pr...
Fábryová, H Celec, P
Published in
Oral diseases
Saliva as a diagnostic fluid enables non-invasive sampling, which can be performed even by an untrained person. Saliva is, thus, particularly useful for large population screenings, for children, elderly and whenever repeated samplings are needed. Saliva is a plasma filtrate actively modified by the salivary glands. Saliva could replace some routin...
