Wu, Zhijie Jin, Yutong Chen, Bifan Gugger, Morgan K. Wilkinson-Johnson, Chance L. Tiambeng, Timothy N. Jin, Song Ge, Ying
Published in
Journal of The American Society for Mass Spectrometry
Reversible phosphorylation plays critical roles in cell growth, division, and signal transduction. Kinases which catalyze the transfer of γ-phosphate groups of nucleotide triphosphates to their substrates are central to the regulation of protein phosphorylation and are therefore important therapeutic targets. Top-down mass spectrometry (MS) present...
Janssen, Kevin A. Coradin, Mariel Lu, Congcong Sidoli, Simone Garcia, Benjamin A.
Published in
Journal of The American Society for Mass Spectrometry
The analysis of histone post-translational modifications (PTMs) by mass spectrometry (MS) has been critical to the advancement of the field of epigenetics. The most sensitive and accurate workflow is similar to the canonical proteomics analysis workflow (bottom-up MS), where histones are digested into short peptides (4-20 aa) and quantitated in ext...
Abdelhamid, Hani Nasser Wu, Hui-Fen
Published in
Journal of The American Society for Mass Spectrometry
The development of simple, low-cost, and specific detection method for mercury (Hg(II)) ions in aqueous media using matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) is a challenge due to matrix interferences and acidity that destroy weak interactions. Herein, a new binary matrix consists of mefenamic acid, and thymine (T) is...
McCool, Elijah N. Lodge, Jean M. Basharat, Abdul Rehman Liu, Xiaowen Coon, Joshua J. Sun, Liangliang
Published in
Journal of The American Society for Mass Spectrometry
Capillary zone electrophoresis (CZE)-tandem mass spectrometry (MS/MS) has been recognized as an efficient approach for top-down proteomics recently for its high-capacity separation and highly sensitive detection of proteoforms. However, the commonly used collision-based dissociation methods often cannot provide extensive fragmentation of proteoform...
Camarillo, Jeannie M. Swaminathan, Suchitra Abshiru, Nebiyu A. Sikora, Jacek W. Thomas, Paul M. Kelleher, Neil L.
Published in
Journal of The American Society for Mass Spectrometry
Histone posttranslational modifications (PTMs) are essential for regulating chromatin and maintaining gene expression throughout cell differentiation. Despite the deep level of understanding of immunophenotypic differentiation pathways in hematopoietic cells, few studies have investigated global levels of histone PTMs required for differentiation a...
Sun, Fangxu Suttapitugsakul, Suttipong Xiao, Haopeng Wu, Ronghu
Published in
Journal of The American Society for Mass Spectrometry
Glycation as a type of non-enzymatic protein modification is related to aging and chronic diseases, especially diabetes. Global analysis of protein glycation will aid in a better understanding of its formation mechanism and biological significance. In this work, we comprehensively investigated protein glycation in human cells (HEK293T, Jurkat, and ...
Haack, Alexander Crouse, Jeff Schlüter, Femke-Jutta Benter, Thorsten Hopkins, W. Scott
Published in
Journal of The American Society for Mass Spectrometry
The use of differential mobility spectrometry (DMS) as a separation tool prior to mass analysis has increased in popularity over the years. However, the fundamental principles behind the difference between high- and low-field mobility is still a matter of debate—especially regarding the strong impact of solvent molecules added to the gas phase in c...
Jin, Yutong Diffee, Gary M. Colman, Ricki J. Anderson, Rozalyn M. Ge, Ying
Published in
Journal of The American Society for Mass Spectrometry
Sarcomeric proteins, including myofilament and Z-disk proteins, play critical roles in regulating muscle contractile properties. A variety of isoforms and post-translational modifications (PTMs) of sarcomeric proteins have been shown to be associated with modulation of muscle functions and the occurrence of muscle diseases. Non-human primates (NHPs...
Cui, Yusi Yang, Ka Tabang, Dylan Nicholas Huang, Junfeng Tang, Weiping Li, Lingjun
Published in
Journal of The American Society for Mass Spectrometry
Simultaneous enrichment of glyco- and phosphopeptides will benefit the studies of biological processes regulated by these posttranslational modifications (PTMs). It will also reveal potential crosstalk between these two ubiquitous PTMs. Unlike custom-designed multifunctional solid phase extraction (SPE) materials, operating strong anion exchange (S...
Javanshad, Roshan Maser, Tara L. Honarvar, Elahe Venter, Andre R.
Published in
Journal of The American Society for Mass Spectrometry
Exposure of electrospray droplets to organic vapors was shown to dramatically reduce alkali-metal adduction on protein ions and shift protein charge states. Since DESI-MS is affected by similar adduct species as ESI-MS and shares similar ionization mechanisms, polar organic vapor additives should likewise also improve the DESI-MS analysis of protei...
