Keller, Andrea-Anneliese Breitling, Reinhard Hemmerich, Peter Kappe, Katarina Braun, Maria Wittig, Berith Schaefer, Buerk Stefan Lorkowski Reissmann, Siegmund
Published in
Journal of Cellular Biochemistry
Cell-penetrating peptides (CPPs) are used to transport peptides, proteins, different types of ribonucleic acids (or mimics of these molecules), and DNA into live cells, both plant and mammalian. Leishmania belongs to the class of protozoa having, in comparison to mammalian cells, a different lipid composition of the membrane, proteoglycans on the s...
Papkoff, J Lai, M H T Tony Hunter
Published in
Journal of Cellular Biochemistry
Pierschbacher, Md Hayman, Eg Erkki Ruoslahti
Published in
Journal of Cellular Biochemistry
Fibronectin possesses a domain that interacts with cell surfaces. The ability of fibronectin to promote cell attachment can be duplicated with a short amino acid sequence, glycyl-L-arginyl-glycyl-L-aspartyl-L-serine, taken from that domain. The tripeptide Arg-Gly-Asp appears to be irreplaceable for maintenance of the activity of this peptide, where...
Lee, Bh Erkki Ruoslahti
Published in
Journal of Cellular Biochemistry
CHO cells expressing alpha5beta1 integrin are more resistant to apoptosis and express more Bcl-2 than the same cells engineered to express alphavbeta1 or cytoplasmically truncated alpha5Deltacbeta1 integrin as their main fibronectin receptor. The Bcl-2 up-regulation by alpha5beta1 is mediated, at least in part, by the focal adhesion kinase (FAK) an...
Köhne, C Johnson, A Tom, S Peers, Dh Gehant, Rl Hotaling, Ta Brousseau, D Ryll, T Fox, Ja Chamow, Sm
...
Published in
Journal of Cellular Biochemistry
Strategies that prevent the attachment of N-linked carbohydrates to nascent glycoproteins often impair intracellular transport and secretion. In the present study, we describe a method to rescue the intracellular transport and secretion of glycoproteins mutagenized to delete N-linked glycosylation sites. Site-directed mutagenesis was used to delete...
Phil Berman Nakamura, Gr Riddle, L Chiu, H Fisher, K Champe, M Gray, Am Ward, P Fong, S
Published in
Journal of Cellular Biochemistry
Full-length (membrane bound) and truncated (secreted) forms of the beta 2 integrin heterodimer, CD11b/CD18 (Mac-1), were expressed in a human kidney cell line (293) that normally does not express leukocyte adhesion molecules (Leu-CAMs). The biosynthesis of recombinant Mac-1 in 293 cells differed from that reported for leukocytes in that heterodimer...
