Lysosomal agents inhibit store-operated Ca 2+ entry
Published in Journal of Cell Science
Summary: Lysosomal agents uncouple ER Ca2+-release from store-operated Ca2+ entry, predominantly by inhibiting Stim1 oligomerization and its activation of Orai.
Mutational inactivation of Apc in the intestinal epithelia compromises cellular organisation
Published in Journal of Cell Science
Highlighted Article: APC control of intestinal epithelia structure and function is regulated through three independent effector pathways specifying cell proliferation, epithelial morphology and intracellular organisation.
Hydrostatic pressure prevents chondrocyte differentiation through heterochromatin remodeling
Published in Journal of Cell Science
Highlighted Article: Hydrostatic pressure promotes chondrocyte quiescence and immature chondrocyte state through reducing the amount of H3K9me3-marked heterochromatin.
A glucose-starvation response governs endocytic trafficking and eisosomal retention of surface cargoes in budding yeast
Published in Journal of Cell Science
Summary: Endocytosis of many cell surface proteins is triggered following glucose starvation, with recovery from starvation mediated by a small fraction of nutrient transporters preserved at the surface in eisosomes.
The association of Plk1 with the Astrin-Kinastrin complex promotes formation and maintenance of a metaphase plate.
Published in Journal of cell science
Errors in mitotic chromosome segregation can lead to DNA damage and aneuploidy, both hallmarks of cancer. To achieve synchronous error-free segregation, mitotic chromosomes must align at the metaphase plate with stable amphitelic attachments to microtubules emanating from opposing spindle poles. The Astrin-Kinastrin/SKAP complex, also containing DY...
Disrupting Polycystin-2 EF hand Ca2+ affinity does not alter channel function or contribute to polycystic kidney disease...
...Published in Journal of cell science
Approximately 15% of autosomal dominant polycystic kidney disease (ADPKD) is caused by variants in PKD2 PKD2 encodes polycystin-2, which forms an ion channel in primary cilia and endoplasmic reticulum (ER) membranes of renal collecting duct cells. Elevated internal Ca2+ modulates polycystin-2 voltage dependent gating and subsequent desensitization-...
The budding yeast Start repressor Whi7 differs in regulation from Whi5, emerging as a major cell cycle brake in response...
Published in Journal of Cell Science
Highlighted Article: Cells can use the interplay between functionally redundant but differentially regulated cell-cycle repressors in order to confer new repression capabilities and to respond to specific cellular conditions.
A piggybacking mechanism enables peroxisomal localization of the glyoxylate cycle enzyme Mdh2 in yeast.
...Published in Journal of cell science
Eukaryotic cells have evolved organelles that allow the compartmentalization and regulation of metabolic processes. Knowledge of molecular mechanisms that allow temporal and spatial organization of enzymes within organelles is therefore crucial for understanding eukaryotic metabolism. Here, we show that the yeast malate dehydrogenase 2 (Mdh2) is du...
Hyperpolarized mitochondria accumulate in Drosophila Hipk-overexpressing cells to drive tumor-like growth.
Published in Journal of cell science
Both functional and dysfunctional mitochondria are known to underlie tumor progression. Here, we establish use of the proto-oncogene Drosophila Homeodomain-interacting protein kinase (Hipk) as a new tool to address this paradox. We find that, in Hipk-overexpressing tumor-like cells, mitochondria accumulate and switch from fragmented to highly fused...
Endophilin-A2-dependent tubular endocytosis promotes plasma membrane repair and parasite invasion
Published in Journal of Cell Science
Summary: Crosstalk between endophilin-A2 and caveolin-1, proteins implicated in clathrin-independent endocytosis, regulates a tubular endocytic pathway important for plasma membrane repair and trypanosome invasion.
