Stuible, Matthew Gervais, Christian Lord-Dufour, Simon Perret, Sylvie L’Abbé, Denis Schrag, Joseph St-Laurent, Gilles Durocher, Yves
Published in
Journal of Biotechnology
Recombinant forms of the spike protein of SARS-CoV-2 and related viruses have proven difficult to produce with good yields in mammalian cells. Given the panoply of potential COVID-19 diagnostic tools and therapeutic candidates that require purified spike protein and its importance for ongoing SARS-CoV-2 research, we have explored new approaches for...
Lothert, Keven Pagallies, Felix Feger, Thomas Amann, Ralf Wolff, Michael W.
Published in
Journal of Biotechnology
In recent years, the Orf virus has become a promising tool for protective recombinant vaccines and oncolytic therapy. However, suitable methods for an Orf virus production, including up- and downstream, are very limited. The presented study focuses on downstream processing, describing the evaluation of different chromatographic unit operations. In ...
Pažitná, Lucia Nemčovič, Marek Pakanová, Zuzana Baráth, Peter Aliev, Teimur Dolgikh, Dmitry Argentova, Victoria Katrlík, Jaroslav
Published in
Journal of Biotechnology
Glycosylation of therapeutic glycoproteins significantly affects their physico-chemical properties, bioactivity and immunogenicity. The determination of glycan composition is highly important regarding their development and production. Therefore, there is a demand for analytical techniques enabling rapid and reliable glycoprofiling of therapeutic p...
Valentina, S. Daniela, B. Mario, C.
Published in
Journal of Biotechnology
Kato, Tatsuya Takami, Yoshihiro Kumar Deo, Vipin Park, Enoch Y
Published in
Journal of biotechnology
Middle East respiratory syndrome coronavirus (MERS-CoV) first emerged in 2012, and over 2000 infections and 800 deaths have been confirmed in 27 countries. However, to date, no commercial vaccine is available. In this study, structural proteins of MERS-CoV were expressed in silkworm larvae and Bm5 cells for the development of vaccine candidates aga...
Gupta, Anjali Mishra, Anuradha Puri, Nidhi
Published in
Journal of biotechnology
Peptide Nucleic Acids (PNAs) are the DNA/RNA analogues in which sugar-phosphate backbone is replaced by N-2-aminoethylglycine repeating units. PNA contains neutral backbone hence due to the absence of electrostatic repulsion, its hybridization shows remarkable stability towards complementary oligonucleotides. PNAs are highly resistant to cleavage b...
Alkhateeb, Rabeaa S. Rückert, Christian Rupp, Oliver Boas Pucker Hublik, Gerd Wibberg, Daniel Niehaus, Karsten Pühler, Alfred Vorhölter, Frank-Jörg
Published in
Journal of Biotechnology
Bioinformatics tools and gene expression data were applied to identify new genes and to enhance the accuracy in genomic feature predictions for Xanthomonas campestris pv. campestris (Xcc) B100, a pathogen of cruciferous plants and model strain for the biosynthesis of xanthan, a polysaccharide with a multitude of commercial applications as a thicken...
Cobzariu, Dragos Baraitareanu, Stelian Leonardi, Leonardo Danes, Doina
Published in
Journal of Biotechnology
Cucos, Catalina Anca Ionaşcu, Iuliana Mocanu, Jacqueline Constantinescu, Radu Militaru, Manuella
Published in
Journal of Biotechnology
Singh-Blom, Amrita Hughes, Randall A. Ellington, Andrew D.
Published in
Journal of Biotechnology
Residue-specific incorporation of non-canonical amino acids into proteins is usually performed in vivo using amino acid auxotrophic strains and replacing the natural amino acid with an unnatural amino acid analog. Herein, we present an efficient amino acid depleted cell-free protein synthesis system that can be used to study residue-specific replac...