Publication search

Rapid, high-yield production of full-length SARS-CoV-2 spike ectodomain by transient gene expression in CHO cells

Published in Journal of Biotechnology

Recombinant forms of the spike protein of SARS-CoV-2 and related viruses have proven difficult to produce with good yields in mammalian cells. Given the panoply of potential COVID-19 diagnostic tools and therapeutic candidates that require purified spike protein and its importance for ongoing SARS-CoV-2 research, we have explored new approaches for...

Selection of chromatographic methods for the purification of cell culture-derived Orf virus for its application as a vac...

Published in Journal of Biotechnology

In recent years, the Orf virus has become a promising tool for protective recombinant vaccines and oncolytic therapy. However, suitable methods for an Orf virus production, including up- and downstream, are very limited. The presented study focuses on downstream processing, describing the evaluation of different chromatographic unit operations. In ...

Influence of media composition on recombinant monoclonal IgA1 glycosylation analysed by lectin-based protein microarray ...

Published in Journal of Biotechnology

Glycosylation of therapeutic glycoproteins significantly affects their physico-chemical properties, bioactivity and immunogenicity. The determination of glycan composition is highly important regarding their development and production. Therefore, there is a demand for analytical techniques enabling rapid and reliable glycoprofiling of therapeutic p...

Laboratory findings and clinical approaches in domestic cats with enteropathies

Published in Journal of Biotechnology

Preparation of virus-like particle mimetic nanovesicles displaying the S protein of Middle East respiratory syndrome cor...

Published in Journal of biotechnology

Middle East respiratory syndrome coronavirus (MERS-CoV) first emerged in 2012, and over 2000 infections and 800 deaths have been confirmed in 27 countries. However, to date, no commercial vaccine is available. In this study, structural proteins of MERS-CoV were expressed in silkworm larvae and Bm5 cells for the development of vaccine candidates aga...

Peptide nucleic acids: Advanced tools for biomedical applications.

Published in Journal of biotechnology

Peptide Nucleic Acids (PNAs) are the DNA/RNA analogues in which sugar-phosphate backbone is replaced by N-2-aminoethylglycine repeating units. PNA contains neutral backbone hence due to the absence of electrostatic repulsion, its hybridization shows remarkable stability towards complementary oligonucleotides. PNAs are highly resistant to cleavage b...

Refined annotation of the complete genome of the phytopathogenic and xanthan producing Xanthomonas campestris pv. camp...

Published in Journal of Biotechnology

Bioinformatics tools and gene expression data were applied to identify new genes and to enhance the accuracy in genomic feature predictions for Xanthomonas campestris pv. campestris (Xcc) B100, a pathogen of cruciferous plants and model strain for the biosynthesis of xanthan, a polysaccharide with a multitude of commercial applications as a thicken...

Feline infectious peritonitis intra vitam diagnosis: Approach and methods

Published in Journal of Biotechnology

Ocular and central nervous system involvement in non-effusive form of feline infectious peritonitis

Published in Journal of Biotechnology

An amino acid depleted cell-free protein synthesis system for the incorporation of non-canonical amino acid analogs into...

Published in Journal of Biotechnology

Residue-specific incorporation of non-canonical amino acids into proteins is usually performed in vivo using amino acid auxotrophic strains and replacing the natural amino acid with an unnatural amino acid analog. Herein, we present an efficient amino acid depleted cell-free protein synthesis system that can be used to study residue-specific replac...