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Development of nucleic acid based lateral flow assays for SARS-CoV-2 detection.

Çam Derin, Dilek Gültekin, Enes İçen Taşkın, Irmak Yakupoğulları, Yusuf

Published in Journal of bioscience and bioengineering

SARS-CoV-2 is still threat for humanity and its detection is crucial. Although real time reverse transcriptase polymerase chain reaction is the most reliable method for detection of N protein genes, alternative methods for molecular detection are still needed. Thus, lateral flow assay models for 2019-nCoV_ N3 were developed for molecular detection....

Rapid and reliable hybridoma screening method that is suitable for production of functional structure-recognizing monocl...

Sakaguchi, Atsumi Nakajima, Chika Sawano, Ayuko Tanaka, Yoichiro Kurihara, Yasuyuki

Published in Journal of Bioscience and Bioengineering

Monoclonal antibodies are extremely valuable functional biomaterials that are widely used not only in life science research but also in antibody drugs and test drugs. There is also a strong need to develop high-quality neutralizing antibodies as soon as possible in order to stop the rapid spread of new infectious diseases such as the SARS-CoV-2 vir...

Dietary trimethylamine N-oxide exacerbates impaired glucose tolerance in mice fed a high fat diet

Gao, Xiang Liu, Xiaofang Xu, Jie Xue, Changhu Xue, Yong Wang, Yuming

Published in Journal of Bioscience and Bioengineering

PLE-wu, a new member of piggyBac transposon family from insect, is active in mammalian cells

Wu, Chunxiao Wang, Shu

Published in Journal of Bioscience and Bioengineering

Molybdenum-containing membrane-bound formate dehydrogenase isolated from Citrobacter sp. S-77 having high stability agai...

Nguyen, Nga T. Yatabe, Takeshi Yoon, Ki-Seok Ogo, Seiji

Published in Journal of Bioscience and Bioengineering

Characterization of α-1,3-glucanase isozyme from Paenibacillus glycanilyticus FH11 in a new subgroup of family 87 α-1,3-...

Suyotha, Wasana Yano, Shigekazu Itoh, Takafumi Fujimoto, Hiroko Hibi, Takao Tachiki, Takashi Wakayama, Mamoru

Published in Journal of Bioscience and Bioengineering

Characterization of human papillomavirus 6b L1 virus-like particles isolated from silkworms using capillary zone electro...

Hahne, Thomas Palaniyandi, Muthukutty Kato, Tatsuya Fleischmann, Peter Wätzig, Hermann Park, Enoch Y.

Published in Journal of Bioscience and Bioengineering

Daptomycin antibiotic production processes in fed-batch fermentation by Streptomyces roseosporus NRRL11379 with precurso...

Ng, I-Son Ye, Chiming Zhang, Zhixiang Lu, Yinghua Jing, Keju

Published in Bioprocess and Biosystems Engineering

Sodium decanoate was first found to be an effective precursor for synthesis of daptomycin from Streptomyces roseosporus NRRL11379 which was increased to 71.55-fold, compared with decanoic acid. The optimal flow rate of precursor was at 600 mg/(L day) after 48 h fermentation. From protein analysis via SDS-PAGE and identification of Tandem MS/MS afte...

Microfluidic perfusion cell culture system confined in 35 mm culture dish for standard biological laboratories

Kondo, Eitaro Wada, Ken-Ichi Hosokawa, Kazuo Maeda, Mizuo

Published in Journal of Bioscience and Bioengineering

Incorporation of adenine nucleotide transporter, Ant1p, into proteoliposomes facilitates ATP translocation and activatio...

Nozawa, Akira Tozawa, Yuzuru

Published in Journal of Bioscience and Bioengineering

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