Mulji, Alpa Haslam, Carl Brown, Fiona Randle, Rebecca Karamshi, Bhumika Smith, Julia Eagle, Robert Munoz-Muriedas, Jordi Taylor, Joanna Sheikh, Arshad
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Published in
Journal of Biomolecular Screening
The biological complexity associated with the regulation of histone demethylases makes it desirable to configure a cellular mechanistic assay format that simultaneously encompasses as many of the relevant cellular processes as possible. In this report, the authors describe the configuration of a JMJD3 high-content cellular mechanistic imaging assay...
Genovesio, Auguste Kwon, Yong-Jun Windisch, Marc P Kim, Nam Youl Choi, Seo Yeon Kim, Hi Chul Jung, Sungyong Fabrizio Mammano Perrin, Virginie Boese, Annette S
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Published in
Journal of Biomolecular Screening
Recent genome-wide RNAi screens have identified >842 human genes that affect the human immunodeficiency virus (HIV) cycle. The list of genes implicated in infection differs between screens, and there is minimal overlap. A reason for this variance is the interdependence of HIV infection and host cell function, producing a multitude of indirect or pl...
Woehrmann, Marcos H Gassner, Nadine C Bray, Walter M Stuart, Joshua M Lokey, Scott
Published in
Journal of biomolecular screening
A high-throughput (HT) agar-based halo assay is described, which allows for rapid screening of chemical libraries for bioactivity in microorganisms such as yeast and bacteria. A pattern recognition algorithm was developed to identify halo-like shapes in plate reader optical density (OD) measurements. The authors find that the total growth inhibitio...
Liu, Yaping Lacson, Raul Cassaday, Jason Ross, David A Kreamer, Anthony Hudak, Edward Peltier, Richard McLaren, Donna Muñoz-Sanjuan, Ignacio Santini, Francesca
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Published in
Journal of Biomolecular Screening
Adult mouse subventricular zone (SVZ) neural stem/progenitor cells are multipotent self-renewing cells that retain the capacity to generate the major cell types of the central nervous system in vitro and in vivo. The relative ease of expanding SVZ cells in culture as neurospheres makes them an ideal model for carrying out large-scale screening to i...
Esposito, Edward A Shrout, Anthony L Weis, Robert M
Published in
Journal of Biomolecular Screening
Receptor tyrosine kinases have become important therapeutic targets because of their involvement in diseases, including cancer. Kinase domains, which are soluble and easily purified, have found widespread use in enzyme inhibitor assays, but these domains do not exhibit full function because they are isolated from the membrane. To address this short...
Ross, D A Lee, S Reiser, V Xue, J Alves, K Vaidya, S Kreamer, A Mull, R Hudak, E Hare, T
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Published in
Journal of Biomolecular Screening
G-protein-coupled receptors (GPCR) participate in many disease pathways and represent the largest family of therapeutic targets. Thus, great investments are made to discover drugs modulating GPCR-mediated events. Among functional assays for screening GPCRs, the Transfluor imaging assay is based on redistribution of cytosolic beta-arrestin to an act...
Gaarde, W A Tony Hunter Brady, H Murray, B W Goldman, M E
Published in
Journal of Biomolecular Screening
Effenberger, Ka Perriman, Rj Bray, Wm Lokey, Rs Ares M, Jr Jurica, Ms
Published in
Journal of Biomolecular Screening
The spliceosome is the macromolecular machine responsible for pre-mRNA splicing, an essential step in eukaryotic gene expression. During splicing, myriad subunits join and leave the spliceosome as it works on the pre-mRNA substrate. Strikingly, there are very few small molecules known to interact with the spliceosome. Splicing inhibitors are needed...
Effenberger, Ka Perriman, Rj Bray, Wm Lokey, Rs Ares M, Jr Jurica, Ms
Published in
Journal of Biomolecular Screening
The spliceosome is the macromolecular machine responsible for pre-mRNA splicing, an essential step in eukaryotic gene expression. During splicing, myriad subunits join and leave the spliceosome as it works on the pre-mRNA substrate. Strikingly, there are very few small molecules known to interact with the spliceosome. Splicing inhibitors are needed...
