Evidence of Slow Motions by Cross-Correlated Chemical Shift Modulation in Deuterated and Protonated Proteins
Similarities between intra- and intermolecular hydrogen bonds in RNA kissing complexes found by means of cross-correlate...
Accurate measurement of residual dipolar couplings in anisotropic phase
Super resolution NOESY spectra of proteins
Published in Journal of Biomolecular NMR
Spectral resolution remains one of the most significant limitations in the NMR study of biomolecules. We present the srNOESY (super resolution nuclear overhauser effect spectroscopy) experiment, which enhances the resolution of NOESY cross-peaks at the expense of the diagonal peak line-width. We studied two proteins, ubiquitin and the influenza hem...
Measurement of residual dipolar couplings in methyl groups via carbon detection
Published in Journal of Biomolecular NMR
Residual dipolar couplings (RDCs) provide both structural and dynamical information useful in the characterization of biological macromolecules. While most data come from the interaction of simple pairs of directly bonded spin-1/2 nuclei (1H–15N, 1H–13C, 1H–1H), it is possible to acquire data from interactions among the multiple spins of 13C-labele...
Selective isotope labeling for NMR structure determination of proteins in complex with unlabeled ligands
Published in Journal of Biomolecular NMR
The physiological role of proteins is frequently linked to interactions with non-protein ligands or posttranslational modifications. Structural characterization of these complexes or modified proteins by NMR may be difficult as the ligands are usually not available in an isotope-labeled form and NMR spectra may suffer from signal overlap. Here, we ...
Maximizing accuracy of RNA structure in refinement against residual dipolar couplings
Published in Journal of Biomolecular NMR
Structural information about ribonucleic acid (RNA) is lagging behind that of proteins, in part due to its high charge and conformational variability. Molecular dynamics (MD) has played an important role in describing RNA structure, complementing information from both nuclear magnetic resonance (NMR), or X-ray crystallography. We examine the impact...
Improving the sensitivity of FT-NMR spectroscopy by apodization weighted sampling
Published in Journal of Biomolecular NMR
Apodization weighted acquisition is a simple approach to enhance the sensitivity of multidimensional NMR spectra by scaling the number of scans during acquisition of the indirect dimension(s). The signal content of the resulting spectra is identical to conventionally sampled data, yet the spectra show improved signal-to-noise ratios. There are no s...
Time-domain signal modelling in multidimensional NMR experiments for estimation of relaxation parameters
Published in Journal of Biomolecular NMR
We present a model-based method for estimation of relaxation parameters from time-domain NMR data specifically suitable for processing data in popular 2D phase-sensitive experiments. Our model is formulated in terms of commutative bicomplex algebra, which allows us to use the complete information available in an NMR signal acquired with principles ...
19F-substituted amino acids as an alternative to fluorophore labels: monitoring of degradation and cellular uptake of an...
Published in Journal of Biomolecular NMR
Current methods for assessment of cellular uptake of cell-penetrating peptides (CPPs) often rely on detection of fluorophore-labeled CPPs. However, introduction of the fluorescent probe often confers changed physicochemical properties, so that the fluorophore-CPP conjugate may exhibit cytotoxic effects and membrane damage not exerted by the native ...
