Anabelle Perrier Bonnin, Ariane Desmarets, Lowiese Danneels, Adeline Goffard, Anne Rouillé, Yves Dubuisson, Jean Belouzard, Sandrine
Published in
Journal of Biological Chemistry
Coronavirus M proteins represent the major protein component of the viral envelope. They play an essential role during viral assembly by interacting with all the other structural proteins. Coronaviruses bud into the endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC), but the mechanisms by which M proteins are transported from their s...
Pertusa, María Rivera, Bastián Gonzalez, Alejandro Ugarte, Gonzalo Madrid, Rodolfo
Published in
Journal of Biological Chemistry
In mammals, the main molecular entity involved in innocuous cold transduction is TRPM8. This polymodal ion channel is activated by cold, cooling compounds such as menthol and voltage. Despite its relevance, the molecular determinants involved in its activation by cold remain elusive. In this study we explored the use of TRPM8 orthologs with differe...
Mathieu, Cécile Bui, Linh-Chi Petit, Emile Haddad, Iman Agbulut, Onnik Vinh, Joelle Rodrigues-Lima, Fernando
Published in
Journal of Biological Chemistry
Dithiocarbamates (DTCs) are important industrial chemicals used extensively as pesticides and in a variety of therapeutic applications. However, they have also been associated with neurotoxic effects and in particular with the development of Parkinson-like neuropathy. Although different pathways and enzymes (such as ubiquitin ligases or the proteas...
Mathieu, Cécile de la Sierra-Gallay, Ines Li Duval, Romain Xu, Ximing Cocaign, Angélique Léger, Thibaut Woffendin, Gary Camadro, Jean-Michel Etchebest, Catherine Haouz, Ahmed
...
Published in
Journal of Biological Chemistry
Brain glycogen metabolism plays a critical role in major brain functions such as learning or memory consolidation. However, alteration of glycogen metabolism and glycogen accumulation in the brain contributes to neurodegeneration as observed in Lafora disease. Glycogen phosphorylase (GP), a key enzyme in glycogen metabolism, catalyzes the rate-limi...
Martin, Katherine R Kantari-Mimoun, Chahrazade Yin, Min Pederzoli-Ribeil, Magali Angelot-Delettre, Fanny Ceroi, Adam Grauffel, Cédric Benhamou, Marc Reuter, Nathalie Saas, Philippe
...
Published in
The Journal of biological chemistry
Proteinase 3 (PR3), the autoantigen in granulomatosis with polyangiitis, is expressed at the plasma membrane of resting neutrophils, and this membrane expression increases during both activation and apoptosis. Using surface plasmon resonance and protein-lipid overlay assays, this study demonstrates that PR3 is a phosphatidylserine-binding protein a...
Butkinaree, Chutikarn Canuel, Maryssa Essalmani, Rachid Poirier, Steve Benjannet, Suzanne Asselin, Marie-Claude Roubtsova, Anna Hamelin, Josée Marcinkiewicz, Jadwiga Chamberland, Ann
...
Published in
Journal of Biological Chemistry
Amyloid precursor-like protein 2 (APLP2) and sortilin were reported to individually bind the proprotein convertase subtilisin/kexin type 9 (PCSK9) and regulate its activity on the low-density lipoprotein receptor (LDLR). The data presented herein demonstrate that mRNA knockdowns of APLP2, sortilin, or both in the human hepatocyte cell lines HepG2 a...
Puto, Lorena A Brognard, John Hunter, Tony
Published in
The Journal of biological chemistry
The DAXX transcriptional repressor was originally associated with apoptotic cell death. However, recent evidence that DAXX represses several tumor suppressor genes, including the DAPK1 and DAPK3 protein kinases, and is up-regulated in many cancers argues that a pro-survival role may predominate in a cancer context. Here, we report that DAXX has pot...
Civra, Andrea Giuffrida, Maria Gabriella Donalisio, Manuela Napolitano, Lorenzo Takada, Yoshikazu Coulson, Barbara S Conti, Amedeo Lembo, David
Published in
The Journal of biological chemistry
Human rotavirus is the leading cause of severe gastroenteritis in infants and children under the age of 5 years in both developed and developing countries. Human lactadherin, a milk fat globule membrane glycoprotein, inhibits human rotavirus infection in vitro, whereas bovine lactadherin is not active. Moreover, it protects breastfed infants agains...
Batchu, Krishna Chaithanya Hokynar, Kati Michael Jeltsch Mattonet, Kenny Somerharju, Pentti
Published in
Journal of Biological Chemistry
The A-type phospholipases (PLAs) are key players in glycerophospholipid (GPL) homeostasis and in mammalian cells; Ca(2+)-independent PLA-β (iPLAβ) in particular has been implicated in this essential process. However, the regulation of this enzyme, which is necessary to avoid futile competition between synthesis and degradation, is not understood. R...
Tenopoulou, Margarita Chen, Jie Jean Bastin Bennett, Michael J Ischiropoulos, Harry Doulias, Paschalis-Thomas
Published in
Journal of Biological Chemistry
Very long acyl-CoA dehydrogenase (VLCAD) deficiency is a genetic pediatric disorder presenting with a spectrum of phenotypes that remains for the most part untreatable. Here, we present a novel strategy for the correction of VLCAD deficiency by increasing mutant VLCAD enzymatic activity. Treatment of VLCAD-deficient fibroblasts, which express disti...
