Analysis of intracellular motion using Kalman filtering
Published in Journal of Biochemical and Biophysical Methods
Methodology to label mixed carbohydrate components by APTS.
Published in Journal of biochemical and biophysical methods
Labelling of carbohydrates with fluorescent dyes is of importance in the analysis of complex oligo- or polysaccharides. The complexity of the hydrolization mixtures of the oligosaccharides can be studied with separation methods after labelling. However, the procedures might provide unexpected phenomena. The experimental circumstances for labelling ...
A new approach for on-line enrichment in electrophoresis of dilute protein solutions.
Published in Journal of biochemical and biophysical methods
A method is described for on-line enrichment/zone sharpening of a sample of negatively charged proteins (an analogous method for cationic proteins can be designed). The sample is applied on the top of a 5-mm thick layer of a neutral polyacrylamide gel which rests on another 5-mm thick, large-pore polyacrylamide gel which contains positively charged...
Determination of the thermodynamics of carbonic anhydrase acid-unfolding by titration calorimetry.
Published in Journal of biochemical and biophysical methods
The enthalpy of unfolding (DeltauH) of carbonic anhydrase II was determined by titrating the protein with acid and measuring the heat using isothermal titration calorimetry (ITC) in the temperature range of 5 to 59 degrees C. By combining the ITC results with our previous findings by differential scanning calorimetry (DSC) in the temperature range ...
A method to increase the bioactivity of plasmid DNA by heat treatment.
Published in Journal of biochemical and biophysical methods
A method to increase the bioactivity of plasmid DNA by heat treatment has been developed. The structure of the heat treated plasmid DNA was investigated by electrophoresis assay and atomic force microscope (AFM) observation. Electrophoresis assay showed that the heat treated DNA consisted of three components: the supercoiled DNA (component I), the ...
The quantitative characterization of free radical sources and traps by electromigration applications.
Published in Journal of biochemical and biophysical methods
Nowadays, very diverse human activities generate urgent demands for fast, sensitive reliable innovative tools capable of detecting major industrial, military, and other dangerous products. An important part of these compounds are free radicals. Capillary electrophoresis (CE), especially in its miniaturized format (lab-on-a-chip), and other electrom...
Exploiting lectin affinity chromatography in clinical diagnosis.
Published in Journal of biochemical and biophysical methods
Lectin affinity chromatography (LAC) offers a tool that aids purification of cell surface glycoconjugates in sufficient quantities so that studies addressing their structural elucidation could be carried out. It has several advantages over the conventional biochemical methods, such as immunoprecipitation and/or immunoaffinity chromatography, used f...
Force microscopy of nonadherent cells: a comparison of leukemia cell deformability.
Published in Journal of Biochemical and Biophysical Methods
