Li, Bin-Bin Chen, Zheng-Yang Jiang, Nan Guo, Song Yang, Jia-Qi Chai, Shao-Bin Yan, Hong-Feng Sun, Pei-Ming Hu, Gang Zhang, Tao
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In Vitro Cellular & Developmental Biology - Animal
Simulated microgravity can significantly affect various cell types and multiple systems of the human body, such as cardiovascular system, skeletal muscle system, and immune system, and is known to cause anemia and loss of electrolyte and fluids. Epidermal stem cells (EpSCs) were cultured in a rotary cell culture system (RCCS) bioreactor to simulate...
Zhao, YanChun Li, Kunlun Zhao, BaoXiang Su, Le
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In Vitro Cellular & Developmental Biology - Animal
In our previous study, we proved that a novel Heat shock protein 90 (HSP90) inhibitor 4-(3-(7-(diethylamino)-2-oxo-2H-chromen-3-yl)-5-phenyl-4,5-dihydro-1H-pyrazol-1-yl) benzoic acid (DPB) could inhibit A549 lung cancer cell growth via inducing apoptosis. However, whether DPB affects autophagy is still unknown. Here, we investigated the effects of ...
Wei, Wenzhen Wang, Gang Cheng, Yunyun Yang, Rui Song, Jie Huang, Shan Li, Haoyang Geng, Hongwei Yu, Hao Liu, Songcai
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In Vitro Cellular & Developmental Biology - Animal
Insulin-like growth factor-1 (IGF-1) is a functional candidate gene for pig growth and development due to its crucial role in the growth axis of growth hormone-IGF-1. Considering that the 3′ untranslated region (3′UTR) of gene may affect its expression, we analyzed the effect of a single-nucleotide polymorphism (SNP) (rs34142920, c.674C > T) on gen...
N., Bhagya Chandrashekar, K. R. Prabhu, Ashwini Rekha, P. D.
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In Vitro Cellular & Developmental Biology - Animal
Tetrandrine is a bisbenzylisoquinoline alkaloid known to exhibit anticancer activity against different cancers. In the present study, the cytotoxic effect of tetrandrine isolated from Cyclea peltata on pancreatic (PANC-1) and breast (MDA-MB-231) cancer cells was evaluated in vitro with an attempt to understand the role of tetrandrine on the generat...
Kang, Dong Young Sp, Nipin Do Park, Kyung Lee, Hak Kyo Song, Ki-Duk Yang, Young Mok
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In Vitro Cellular & Developmental Biology - Animal
Ketosis is a condition where ketone bodies are produced as an alternative energy source, due to insufficient glucose for energy production so that the body switches from carbohydrate metabolism to mostly fat metabolism. In this study, we examined the anti-ketosis effects of silibinin, a major active component of silymarin. We induced ketosis in FL8...
Li, Mingyi Luo, Ruolan Yang, Wenjian Zhou, Zhen Li, Chenxia
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In Vitro Cellular & Developmental Biology - Animal
Osteoporosis results from the imbalance between osteogenesis and bone resorption mediated by osteoblasts and osteoclasts. During the disease process of osteoporosis, the alteration of gene expression occurs, which lead to the disease progression. MicroRNAs (miRNAs) have been previously demonstrated to be modulators for bone metabolism via regulatio...
Liao, Zhihong Dai, Zhenkai Cai, Chenyu Zhang, Xinheng Li, Aijun Zhang, Huanmin Yan, Yiming Lin, Wencheng Wu, Yu Li, Hongxin
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In Vitro Cellular & Developmental Biology - Animal
Atg5, as a switch of cell autophagy and apoptosis, plays an important regulatory role in the occurrence and development of autophagy. Atg5 has been reported to involve the autophagy process but little in the apoptotic process. Here, we constructed an Atg5−/− DF-1 cell line using the CRISPR/Cas9 assay and confirmed the significant difference in grow...
Takekoshi, Daisuke Tokuzawa, Yoshimi Sakanaka, Masahiro Kato, Hidemasa
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In Vitro Cellular & Developmental Biology - Animal
N-terminal acetylation (Nt-acetylation) refers to the acetylation of the free α-amino group at the N-terminus of a polypeptide. While the effects of Nt-acetylation are multifaceted, its most known function is in the acetylation-dependent N-end rule protein degradation pathway (Ac/N-end rule pathway), where Nt-acetylation is recognized as a degron b...
Safavi, Atiyeh Sadat Rouhi, Gholamreza Haghighipour, Nooshin Bagheri, Fatemeh Eslaminejad, Mohamadreza Baghaban Sayahpour, Frough Azam
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In Vitro Cellular & Developmental Biology - Animal
This study aimed at investigating the expression of osteoblast and chondrocyte-related genes in mesenchymal stem cells (MSCs), derived from rabbit adipose tissue, under mechanical vibration. The cells were placed securely on a vibrator’s platform and subjected to 300 Hz of sinusoidal vibration, with a maximum amplitude of 10 μm, for 45 min per day,...
Talbot, Neil C. Shannon, Amy E. Garrett, Wesley M.
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In Vitro Cellular & Developmental Biology - Animal
The isolation of a cell line, PICM-31D, with phenotypic characteristics like pancreatic duct cells is described. The PICM-31D cell line was derived from the previously described pig embryonic stem cell-derived exocrine pancreatic cell line, PICM-31. The PICM-31D cell line was morphologically distinct from the parental cells in growing as a monolaye...
