Lasson, A Balldin, G Ohlsson, K
Published in
Hoppe-Seyler's Zeitschrift für physiologische Chemie
The effect of gabexate mesilate (FOY) was studied in vitro in human and canine serum upon the addition of trypsin, and in vivo in dogs during intravenous trypsin infusion. The effect of FOY was compared with the effect of aprotinin. FOY did not show any protection against trypsin-induced activation of the complement and kinin systems in vitro or in...
Nöhle, U Schauer, R
Published in
Hoppe-Seyler's Zeitschrift für physiologische Chemie
A mixture of N-acetyl-[4,5,6,7,8,9-14C]neuraminosyl-alpha (2-3(6]-galactosyl-beta (1-4-glucose[( 14C]sialyl-lactose) and N-acetylneuraminosyl-alpha (2-3(6]-galactosyl-beta(1-4)-glucit-1-[3H]ol(sialyl-[3H]lactitol) as well as porcine submandibular gland mucin labeled with N-acetyl- and N-glycoloyl-[9-(3)H]neuraminic acid were administered orally to ...
Schubert, D Maurer, E Boss, K Pfleiderer, G
Published in
Hoppe-Seyler's Zeitschrift für physiologische Chemie
The self-association of glucose dehydrogenase (beta-D-glucose:NAD(P) 1-oxidoreductase, EC 1.1.1.47) from Bacillus megaterium was studied by analytical ultracentrifugation. The pH and composition of the buffer used were such that, owing to a reversible partial dissociation of the tetrameric enzyme, enzyme activity was reduced. It was found that unde...
Kasche, V Haufler, U Zöllner, R
Published in
Hoppe-Seyler's Zeitschrift für physiologische Chemie
Hydrophobic protein chromatography was used to prepare homogeneous fractions of penicillin amidase (EC 3.5.1.11) from E. coli. The apparent ratios of the rate constants for the deacylation of the acyl-penicillin amidase formed in the hydrolysis of phenylacetylglycine or D-phenylglycine methyl ester, by H2O and 6-aminopenicillanic acid (6-APA), were...
Hallenbach, W Horner, L
Published in
Hoppe-Seyler's Zeitschrift für physiologische Chemie
The inhibition of acetylcholinesterase with fluorophores type II and III linked with the OH-selective fluorophosphonoyl groups is kinetically investigated by comparing the changes in activity and fluorescence. The hydrolysis of the fluorescent phosphonoylfluorides 1 and 4 to 7 in aqueous buffer solutions does not interfere with the inhibition kinet...
Aebersold, R Herbst, H Grütter, T Chang, J Y Braun, D G
Published in
Hoppe-Seyler's Zeitschrift für physiologische Chemie
Antibodies 17S29.1 and 22S25.1 are monoclonal, hybridoma-derived gamma 3 kappa murine immunoglobulins with specificity for N-acetyl-glucosamine beta 1----3-linked to the L-rhamnose backbone structure, the immunodeterminant of the streptococcal Group A polysaccharide. The VL 17S29.1 amino-acid sequence is the third complete one reported from an anti...
Aebersold, R Herbst, H Chang, J Y Braun, D G
Published in
Hoppe-Seyler's Zeitschrift für physiologische Chemie
Antibody 50S10.1 is a hybridoma-derived gamma 3 kappa antibody of BAB-14 mouse strain origin, with specificity for N-acetylglucosamine beta 1----3 linked to L-rhamnose, the immunodeterminant of the streptococcal Group A polysaccharide. The VL50S10.1 amino acid sequence is the fourth complete one reported with this specificity and the first fully de...
Eiffert, H Quentin, E Decker, J Hillemeir, S Hufschmidt, M Klingmüller, D Weber, M H Hilschmann, N
Published in
Hoppe-Seyler's Zeitschrift für physiologische Chemie
The amino-acid sequence and the arrangement of the disulfide bonds of the human free secretory component were completely elucidated by the methods of protein chemistry. The free secretory component is a monomeric glycoprotein (Mr approximately 86000), consisting of 558 amino acids with 7 carbohydrate chains bound to asparagine. The protein contains...
Williams, J F Clark, M G Arora, K K Reichstein, I C
Published in
Hoppe-Seyler's Zeitschrift für physiologische Chemie
An investigation of the mechanism in vitro of the non-oxidative segment of the pentose phosphate pathway using [5-14C]ribose 5-phosphate as a prediction labelling substrate with rat liver enzyme preparation is reported. Glucose 6-phosphate formed during the initial 0.5 h of reaction was heavily labelled in C-1 and thus is consistent with the predic...
Zachau, H G Pech, M Klobeck, H G Pohlenz, H D Straubinger, B Falkner, F G
Published in
Hoppe-Seyler's Zeitschrift für physiologische Chemie
