Eiffert, H Quentin, E Decker, J Hillemeir, S Hufschmidt, M Klingmüller, D Weber, M H Hilschmann, N
Published in
Hoppe-Seyler's Zeitschrift für physiologische Chemie
The amino-acid sequence and the arrangement of the disulfide bonds of the human free secretory component were completely elucidated by the methods of protein chemistry. The free secretory component is a monomeric glycoprotein (Mr approximately 86000), consisting of 558 amino acids with 7 carbohydrate chains bound to asparagine. The protein contains...
Williams, J F Clark, M G Arora, K K Reichstein, I C
Published in
Hoppe-Seyler's Zeitschrift für physiologische Chemie
An investigation of the mechanism in vitro of the non-oxidative segment of the pentose phosphate pathway using [5-14C]ribose 5-phosphate as a prediction labelling substrate with rat liver enzyme preparation is reported. Glucose 6-phosphate formed during the initial 0.5 h of reaction was heavily labelled in C-1 and thus is consistent with the predic...
Zachau, H G Pech, M Klobeck, H G Pohlenz, H D Straubinger, B Falkner, F G
Published in
Hoppe-Seyler's Zeitschrift für physiologische Chemie
Schubert, D Maurer, E Boss, K Pfleiderer, G
Published in
Hoppe-Seyler's Zeitschrift für physiologische Chemie
The self-association of glucose dehydrogenase (beta-D-glucose:NAD(P) 1-oxidoreductase, EC 1.1.1.47) from Bacillus megaterium was studied by analytical ultracentrifugation. The pH and composition of the buffer used were such that, owing to a reversible partial dissociation of the tetrameric enzyme, enzyme activity was reduced. It was found that unde...
Nöhle, U Schauer, R
Published in
Hoppe-Seyler's Zeitschrift für physiologische Chemie
A mixture of N-acetyl-[4,5,6,7,8,9-14C]neuraminosyl-alpha (2-3(6]-galactosyl-beta (1-4-glucose[( 14C]sialyl-lactose) and N-acetylneuraminosyl-alpha (2-3(6]-galactosyl-beta(1-4)-glucit-1-[3H]ol(sialyl-[3H]lactitol) as well as porcine submandibular gland mucin labeled with N-acetyl- and N-glycoloyl-[9-(3)H]neuraminic acid were administered orally to ...
Aebersold, R Herbst, H Chang, J Y Braun, D G
Published in
Hoppe-Seyler's Zeitschrift für physiologische Chemie
Antibody 50S10.1 is a hybridoma-derived gamma 3 kappa antibody of BAB-14 mouse strain origin, with specificity for N-acetylglucosamine beta 1----3 linked to L-rhamnose, the immunodeterminant of the streptococcal Group A polysaccharide. The VL50S10.1 amino acid sequence is the fourth complete one reported with this specificity and the first fully de...
Conti, A Godovac-Zimmermann, J Liberatori, J Braunitzer, G
Published in
Hoppe-Seyler's Zeitschrift für physiologische Chemie
beta-Lactoglobulin-like proteins were detected in horse colostrum and normal milk using immunological techniques. In contrast to the beta-lactoglobulins sequenced so far these proteins are monomeric and genetically not homogenous. In this paper we report the first primary structure of a monomeric beta-lactoglobulin from horse colostrum. By means of...
Hallenbach, W Horner, L
Published in
Hoppe-Seyler's Zeitschrift für physiologische Chemie
The inhibition of acetylcholinesterase with fluorophores type II and III linked with the OH-selective fluorophosphonoyl groups is kinetically investigated by comparing the changes in activity and fluorescence. The hydrolysis of the fluorescent phosphonoylfluorides 1 and 4 to 7 in aqueous buffer solutions does not interfere with the inhibition kinet...
Odum, L Byrjalsen, I
Published in
Hoppe-Seyler's Zeitschrift für physiologische Chemie
By means of a sensitive electrophoretic technique for the detection of proteinase inhibitors three slowly migrating proteinase inhibitors (SMPI) were discovered in some samples of pathological urine. SMPI 1 migrated in the beta 2-zone whereas SMPI 2 and SMPI 3 appeared in the anodal and cathodal gamma-zone, respectively. Only SMPI 1 and 2 were exam...
Ziegler, R Kegel, G Keller, R
Published in
Hoppe-Seyler's Zeitschrift für physiologische Chemie
An adipokinetic hormone was purified from corpora cardiaca of au adult Manduca sexta (tobacco hornworm)by reversed-phased high performance liquid chromatography. The following amino-acid composition was determined: 2 Thr, 2 Ser, Glx, Gly, Leu, Phe, Trp. The adipokinetic activity was tested in adult Manduca. In a bioassay in which activation of fat ...
