Hasehira, Kayo Hirabayashi, Jun Tateno, Hiroaki
Published in
Glycoconjugate journal
Human somatic stem cells such as mesenchymal stem cells (hMSCs) have the capacity to differentiate into mesenchymal tissue lineages and to alter immune regulatory functions. As such, they hold promise for use in stem cell-based therapies. However, no method is currently available to evaluate the actual differentiation capacity of hMSCs prior to cel...
Breimer, Michael E Säljö, Karin Barone, Angela Teneberg, Susann
Published in
Glycoconjugate journal
The application of human stem cell technology offers theoretically a great potential to treat various human diseases. However, to achieve this goal a large number of scientific issues remain to be solved. Cell surface carbohydrate antigens are involved in a number of biomedical phenomena that are important in clinical applications of stem cells, su...
Muramatsu, Takashi
Published in
Glycoconjugate journal
Embryonal carcinoma cells, stem cells of teratocarcinomas, are pluripotent stem cells and also prototypes of embryonic stem cells. Embryonal carcinoma cells contain large amounts of a highly branched poly-N-acetyllactosamine called embryoglycan, which has a molecular weight of approximately 10,000 or greater, and is asparagine-linked. This glycan w...
Yagi, Hirokazu Kato, Koichi
Published in
Glycoconjugate journal
Neural stem cells (NSCs) possess a high proliferative potential and capacity for self-renewal with retention of multipotency to differentiate into brain-forming cells. NSCs have gained a considerable attention because of their potential application in treatment strategies on the basis of transplantation for neurodegenerative disorders and nerve inj...
Handa, Kazuko Hakomori, Sen-Itiroh
Published in
Glycoconjugate journal
A variety of glycoconjugates, including glycosphingolipids (GSLs), expressed in mammalian tissues and cells were isolated and characterized in early biochemical studies. Later studies of virus-transformed fibroblasts demonstrated the association of GSL expression profiles with cell phenotypes. Changes of GSL expression profile were observed during ...
Wang, Haisheng He, Wenqin Jiang, Peixia Yu, Yanlei Lin, Lei Sun, Xiaojun Koffas, Mattheos Zhang, Fuming Linhardt, Robert J
Published in
Glycoconjugate journal
There is a need for degradative enzymes in the study of glycosaminoglycans. Many of these enzymes are currently available either in their natural or recombinant forms. Unfortunately, progress in structure-activity studies of keratan sulfate (KS) have been impeded by the lack of a commercially available endo-β-N-acetylglucosaminidase, keratantase II...
Bradley, Stefanie S Dick, Morag F Guglielmo, Christopher G Timoshenko, Alexander V
Published in
Glycoconjugate journal
Galectins, a family of multifunctional glycan-binding proteins, are proposed as biomarkers of cellular stress responses. Avian migration is an energetically challenging physical stress, which represents a physiological model of muscular endurance exercises. This study assesses change in galectin gene expression profiles associated with seasonal var...
Neelofar, Km Ahmad, Jamal
Published in
Glycoconjugate journal
Non-enzymatic glycation of macromolecules, especially proteins leading to their oxidation is increased in diabetes mellitus due to hyperglycaemia and play an important role in associated complications of the disease. Protein glycation mostly occurs in intra chain lysine residues resulting in the formation of early stage Amadori products which are f...
Usvalampi, Anne Maaheimo, Hannu Tossavainen, Olli Frey, Alexander D
Published in
Glycoconjugate journal
Fucosylated oligosaccharides have an important role in maintaining a healthy immune system and homeostatic gut microflora. This study employed a commercial β-galactosidase in the production of fucose-containing galacto-oligosaccharides (fGOS) from lactose and fucose. The production was optimized using experiment design and optimal conditions for a ...
Abrahams, Jodie L Campbell, Matthew P Packer, Nicolle H
Published in
Glycoconjugate journal
Porous graphitised carbon-liquid chromatography (PGC-LC) has been proven to be a powerful technique for the analysis and characterisation of complex mixtures of isomeric and isobaric glycan structures. Here we evaluate the elution behaviour of N-glycans on PGC-LC and thereby provide the potential of using chromatographic separation properties, toge...
