Cox, A D St Michael, F Aubry, A Strong, P C R Hayes, A C Logan, S M
Published in
Glycoconjugate journal
Two known Clostridiodes (Clostridium) difficile surface antigens, a lipoteichoic acid (LTA) and a polysaccharide (PS-II) were isolated and purified in order to prepare glycoconjugate vaccines to the carrier protein human serum albumin utilising a reductive amination strategy. Mice and rabbits were immunized with a prime and two boost strategy and t...
Nandi, Sandip K Rankenberg, Johanna Rakete, Stefan Nahomi, Rooban B Glomb, Marcus A Linetsky, Mikhail D Nagaraj, Ram H
Published in
Glycoconjugate journal
Proteins in the eye lens have negligible turnover and therefore progressively accumulate chemical modifications during aging. Carbonyls and oxidative stresses, which are intricately linked to one another, predominantly drive such modifications. Oxidative stress leads to the loss of glutathione (GSH) and ascorbate degradation; this in turn leads to ...
Leerach, Nontaphat Harashima, Ai Munesue, Seiichi Kimura, Kumi Oshima, Yu Goto, Hisanori Yamamoto, Hiroshi Higashida, Haruhiro Yamamoto, Yasuhiko
Published in
Glycoconjugate journal
The receptor for advanced glycation end-products (receptor for AGEs, RAGE) is a pattern recognition receptor. The interaction of RAGE with its ligands, such as AGEs, S100 proteins, high mobility group box-1 (HMGB1), and lipopolysaccharides (LPS), is known to play a pivotal role in the propagation of immune responses and inflammatory reactions. The ...
Yamaguchi, Hiroko Nagai, Mime Sugawa, Hikari Yasuda, Hisataka Nagai, Ryoji
Published in
Glycoconjugate journal
Methylglyoxal (MGO) produced during glycolysis is known to react with arginine residues on proteins to generate advanced glycation end products, such as Nδ-(5-hydro-5-methyl-4-imidazolone-2-yl)-ornithine (MG-H1). Since the production of MGO is increased during hyperglycemia or metabolic disorders in vivo, it is considered that the measurement of MG...
Gensberger-Reigl, Sabrina Auditore, Andrea Huppert, Jochen Pischetsrieder, Monika
Published in
Glycoconjugate journal
Heat sterilization of peritoneal dialysis fluids (PDFs) leads to the formation of glucose degradation products (GDPs), which impair long-term peritoneal dialysis. The current study investigated the effects of metal ions, which occur as trace impurities in the fluids, on the formation of six major α-dicarbonyl GDPs, namely glucosone, glyoxal, methyl...
Rabbani, Naila Xue, Mingzhan Thornalley, Paul J
Published in
Glycoconjugate journal
The reactive dicarbonyl metabolite, methylglyoxal (MG), is increased in obesity and diabetes and is implicated in the development of insulin resistance, type 2 diabetes mellitus and vascular complications of diabetes. Dicarbonyl stress is the metabolic state of abnormal high MG concentration. MG is an arginine-directed glycating agent and precursor...
Tessier, Frederic J Boulanger, Eric Howsam, Michael
Published in
Glycoconjugate journal
The Maillard reaction, also called glycation, is one of the major chemical reactions responsible for most yellow-to-brown colors and aromas in cooked foods. This reaction between reducing sugars and amino functions on proteins affects not only the flavor of food, but also leads to the formation of an heterogenous group of structurally-modified amin...
Murata, Masatsune
Published in
Glycoconjugate journal
The Maillard reaction was discovered in 1912 by Louis C. Maillard when he observed the browning phenomena with aroma formation in a heated solution containing a sugar and an amino acid. The Maillard reaction starts from the reactions between carbonyl groups of various sugars and amino groups of amino acids/ proteins, following the formation of inte...
Pongracz, Tamas Verhoeven, Aswin Wuhrer, Manfred de Haan, Noortje
Published in
Glycoconjugate journal
Sialic acids occur ubiquitously throughout vertebrate glycomes and often endcap glycans in either α2,3- or α2,6-linkage with diverse biological roles. Linkage-specific sialic acid characterization is increasingly performed by mass spectrometry, aided by differential sialic acid derivatization to discriminate between linkage isomers. Typically, duri...
Huang, Shih-Pei Hsu, Hsu Chen Liew, Chia Yen Tsai, Shang-Ting Ni, Chi-Kung
Published in
Glycoconjugate journal
Mass spectrometry has high sensitivity and is widely used in the identification of molecular structures, however, the structural determination of oligosaccharides through mass spectrometry is still challenging. A novel method, namely the logically derived sequence (LODES) tandem mass spectrometry (MSn), for the structural determination of underivat...
