Huang, Shih-Pei Hsu, Hsu Chen Liew, Chia Yen Tsai, Shang-Ting Ni, Chi-Kung
Published in
Glycoconjugate journal
Mass spectrometry has high sensitivity and is widely used in the identification of molecular structures, however, the structural determination of oligosaccharides through mass spectrometry is still challenging. A novel method, namely the logically derived sequence (LODES) tandem mass spectrometry (MSn), for the structural determination of underivat...
Yang, Shuang Wang, Yan Mann, Matthew Wang, Qiong Tian, E Zhang, Liping Cipollo, John F Ten Hagen, Kelly G Tabak, Lawrence A
Published in
Glycoconjugate journal
O-glycosylation is a highly diverse and complex form of protein post-translational modification. Mucin-type O-glycosylation is initiated by the transfer of N-acetyl-galactosamine (GalNAc) to the hydroxyl group of serine, threonine and tyrosine residues through catalysis by a family of glycosyltransferases, the UDP-GalNAc:polypeptide N-acetylgalacto...
Kuttel, Michelle M Berti, Francesco Ravenscroft, Neil
Published in
Glycoconjugate journal
Vaccination is the most cost-effective way to control disease caused by encapsulated bacteria; the capsular polysaccharide (CPS) is the primary virulence factor and vaccine target. Neisseria meningitidis (Nm) serogroups B, C, Y and W all contain sialic acid, a common surface feature of human pathogens. Two protein-based vaccines against serogroup B...
Cao, Ran Zhang, Tian-Chan Chen, Ya-Ran Cao, Cui Chen, Huan Huang, Yi-Fan Fujita, Morihisa Liu, Li Voglmeir, Josef
Published in
Glycoconjugate journal
β1,4-GalT1 is a type II membrane glycosyltransferase. It catalyzes the production of lactose in the lactating mammary gland and is supposedly also involved in the galactosylation of terminal GlcNAc of complex-type N-glycans. In-vitro studies of the bovine β4Gal-T1 homolog showed that replacing a single residue of tyrosine with leucine at position 2...
An, So-Young Lee, Miri Yoon, Hyun-Kyoung Abekura, Fukushi Kim, Kyoung-Sook Kim, Dong-Hyun Kim, Hyeon-Jun Lee, Kichoon Kim, Cheorl-Ho Lee, Young-Choon
...
Published in
Glycoconjugate journal
In this study, we found that gene expression of the human β-galactoside α2,6-sialyltransferase (hST6Gal I) was specifically increased during differentiation of human MG-63 osteoblastic cells by serum starvation (SS). In parallel, a distinct increase in binding to SNA, the α2,6-sialyl-specific lectin, was observed in serum-starved cells, as demonstr...
Leerach, Nontaphat Harashima, Ai Munesue, Seiichi Kimura, Kumi Oshima, Yu Goto, Hisanori Yamamoto, Hiroshi Higashida, Haruhiro Yamamoto, Yasuhiko
Published in
Glycoconjugate journal
The receptor for advanced glycation end-products (receptor for AGEs, RAGE) is a pattern recognition receptor. The interaction of RAGE with its ligands, such as AGEs, S100 proteins, high mobility group box-1 (HMGB1), and lipopolysaccharides (LPS), is known to play a pivotal role in the propagation of immune responses and inflammatory reactions. The ...
Pither, Molly D McClean, Siobhán Silipo, Alba Molinaro, Antonio Di Lorenzo, Flaviana
Published in
Glycoconjugate journal
Pandoraea sp. is an emerging Gram-negative pathogen in cystic fibrosis causing severe and persistent inflammation and damage of the lungs. The molecular mechanisms underlying the high pathogenicity of Pandoraea species are still largely unknown. As Gram-negatives, Pandoraea sp. express lipopolysaccharides (LPS) whose recognition by the host immune ...
Minakata, Shiho Inai, Yoko Manabe, Shino Nishitsuji, Kazuchika Ito, Yukishige Ihara, Yoshito
Published in
Glycoconjugate journal
C-Mannosyl tryptophan (C-Man-Trp) is a unique glycosylated amino acid present in various eukaryotes. The C-Man-Trp structure can be found as a monomeric form or a part of post-translational modifications within polypeptide chains in living organisms. However, the mechanism of how monomeric C-Man-Trp is produced has not been fully investigated. In t...
Yan, Lufeng Brodfueher, Paul Fu, Li Zhang, Fuming Chen, Shiguo Dordick, Jonathan S Linhardt, Robert J
Published in
Glycoconjugate journal
Heparosan, the capsular polysaccharide of E. coli K5 is currently used as the starting material in the chemoenzymatic synthesis of heparan sulfate and the structurally related anticoagulant drug heparin. Base hydrolysis of N-acetyl groups and their subsequent N-sulfonation, are used to prepare N-sulfoheparosan an intermediate of biosynthesis. In th...
Liu, Chao Ji, Hai-Yu Wu, Peng Yu, Juan Liu, An-Jun
Published in
Glycoconjugate journal
In this study, a novel water soluble polysaccharide (named GFP-4) was extracted from Grifola frondosa at 4 oC, and its preliminary structure and inhibitory effects on human gastric carcinoma MKN-45 cells through the Fas/FasL death receptor apoptosis pathway were investigated. High-performance gel permeation chromatography (HPGPC), fourier-transform...
