Usvalampi, Anne Ruvalcaba Medrano, Marcela Maaheimo, Hannu Salminen, Heidi Tossavainen, Olli Frey, Alexander D.
Published in
Glycoconjugate Journal
Fucosylated oligosaccharides are interesting molecules due to their bioactive properties. In particular, their application as active ingredient in milk powders is attractive for dairy industries. The objective of this study was to characterize the glycosyl hydrolase family 29 α-fucosidase produced by Aspergillus niger and test its ability to transf...
Nakamura, Yuichiro Miyata, Yasuyoshi Matsuo, Tomohiro Shida, Yohei Hakariya, Tomoaki Ohba, Kojiro Taima, Takenobu Ito, Akihiro Suda, Tetsuji Hakomori, Sen-Itiroh
...
Published in
Glycoconjugate journal
Stage-specific embryonic antigen-4 (SSEA-4), a specific marker for pluripotent stem cells, plays an important role in the malignant behavior of several cancers. Here, SSEA-4 expression was evaluated by immunohistochemistry using monoclonal antibody RM1 specific to SSEA-4 in 181 and 117 prostate cancer (PC) specimens obtained by biopsy and radical p...
Turupcu, Aysegül Poliak, Peter Margreitter, Christian Oostenbrink, Chris Staudacher, Erika
Published in
Glycoconjugate Journal
UDP-GalNAc:polypeptide GalNAc transferase (ppGalNAcT; EC 2.4.1.41) is the initiating enzyme for mucin-type O-glycosylation in animals. Members of this highly conserved glycosyltransferase family catalyse a single glycosidic linkage. They transfer an N -acetylgalactosamine (GalNAc) residue from an activated donor (UDP-GalNAc) to a serine or threonin...
Paschinger, Katharina Wilson, Iain B. H.
Published in
Glycoconjugate Journal
Glycan structures in non-vertebrates are highly variable; it can be assumed that this is a product of evolution and speciation, not that it is just a random event. However, in animals and protists, there is a relatively limited repertoire of around ten monosaccharide building blocks, most of which are neutral in terms of charge. While two monosacch...
Du, Jiayan Liu, Su Liang, Quntao Lin, Jianghui Jiang, Lilong Chen, Fener Wei, Zheng
Published in
Glycoconjugate Journal
Colla corii asini (CCA) made from donkey-hide has been widely used as a health-care food and an ingredient of traditional Chinese medicine. Heparan sulfate (HS)/heparin is a structurally complex class of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities. However, their presence within CCA, and their possib...
Tanaka, Kyoko Suzuki, Akemi Aoki, Daisuke Iwamori, Masao
Published in
Glycoconjugate Journal
Humanized monoclonal antibody HMMC-1 established by immunizing transchromosomal mice with a human uterine endometrial cancer cell line has been found to react with the H-antigen carried on core l O-glycans through cotransfection of glycosyltransferases for O-glycans and inhibition of antibody-binding with synthetic oligosaccharides. However, direct...
Matsuoka, Yuko Kawauchi, Yoko Kawauchi, Kiyotaka Takiyama, Akari Kojima, Shohei Kuroda, Yasuhiro Kojima, Naoya
Published in
Glycoconjugate Journal
In this study, we assessed the potential of liposomes coated with a neoglycolipid containing α1–3,α1–6-mannotriose residues (Man3-DPPE; Manα1–6(Manα1–3)Manitol-DPPE) for in vitro activation and maturation of human mononuclear phagocytes. In response to treatment with Man3-DPPE-coated liposomes (Man3-OMLs), PMA-stimulated human THP-1 cells showed en...
Hof, Danique J. Versteeg, Elly M. M. van de Lest, Chris H. A. Daamen, Willeke F. van Kuppevelt, Toin H.
Published in
Glycoconjugate Journal
Glycosaminoglycans (GAGs) are known to play pivotal roles in physiological processes and pathological conditions. To study interactions of GAGs with proteins, immobilization of GAGs is often required. Current methodologies for immobilization involve modification of GAGs and/or surfaces, which can be time-consuming and may involve specialized equipm...
Oh, So-Young Park, Myeong-Soo Lee, Yeong-Geun Thi, Nhan Nguyen Baek, Nam-In Ji, Geun-Eog
Published in
Glycoconjugate Journal
Breast-fed infants have Bifidobacterium-rich gut microbiota compared to infants fed formula. Fucosylated oligosaccharides are the major components of human milk oligosaccharide (HMO) which confer various beneficial effects including prebiotic effect and protection from pathogenic infection on the host. A novel prebiotics was developed using bifidob...
Wu, Albert M. Liu, Jia Hau
Published in
Glycoconjugate Journal
Lectins, in combination with our established enzyme-linked lectin sorbent assay (ELLSA) and inhibition study, have been used as powerful tools in many glycoconjugate recognition studies. In this short review, we highlight the following: (i) The recognition profiles of Gal/GalNAc-specific lectins were updated and upgraded. (ii) Based on the cross-sp...
