Wei, Yu-Miao Li, Xiang Xu, Ming Abais, Justine M. Chen, Yang Riebling, Christopher R. Boini, Krishna M. Li, Pin-Lan Zhang, Yang
Published in
Cellular Physiology and Biochemistry
Background/Aims: In addition to their action of lowering blood cholesterol levels, statins modulate biological characteristics and functions of arterial myocytes such as viability, proliferation, apoptosis, survival and contraction. The present study tested whether simvastatin, as a prototype statin, enhances autophagy in coronary arterial myocytes...
Latorre, Isabel Chesney, Michael A Garrigues, Jacob M Stempor, Przemyslaw Appert, Alex Francesconi, Mirko Strome, Susan Ahringer, Julie
Published in
Genes & development
The DREAM (DP, Retinoblastoma [Rb]-like, E2F, and MuvB) complex controls cellular quiescence by repressing cell cycle genes, but its mechanism of action is poorly understood. Here we show that Caenorhabditis elegans DREAM targets have an unusual pattern of high gene body HTZ-1/H2A.Z. In mutants of lin-35, the sole p130/Rb-like gene in C. elegans, D...
Burke, Jason R Hura, Greg L Rubin, Seth M
Published in
Genes & development
Cyclin-dependent kinase (Cdk) phosphorylation of the Retinoblastoma protein (Rb) drives cell proliferation through inhibition of Rb complexes with E2F transcription factors and other regulatory proteins. We present the first structures of phosphorylated Rb that reveal the mechanism of its inactivation. S608 phosphorylation orders a flexible "pocket...
Gehman, Lauren T Meera, Pratap Stoilov, Peter Shiue, Lily O'Brien, Janelle E Meisler, Miriam H Ares, Manuel Jr Otis, Thomas S Black, Douglas L
Published in
Genes & development
The Rbfox proteins (Rbfox1, Rbfox2, and Rbfox3) regulate the alternative splicing of many important neuronal transcripts and have been implicated in a variety of neurological disorders. However, their roles in brain development and function are not well understood, in part due to redundancy in their activities. Here we show that, unlike Rbfox1 dele...
Nimonkar, Amitabh V Genschel, Jochen Kinoshita, Eri Polaczek, Piotr Campbell, Judith L Wyman, Claire Modrich, Paul Kowalczykowski, Stephen C
Published in
Genes & development
Repair of dsDNA breaks requires processing to produce 3'-terminated ssDNA. We biochemically reconstituted DNA end resection using purified human proteins: Bloom helicase (BLM); DNA2 helicase/nuclease; Exonuclease 1 (EXO1); the complex comprising MRE11, RAD50, and NBS1 (MRN); and Replication protein A (RPA). Resection occurs via two routes. In one, ...
Moss, Jennifer Tinline-Purvis, Helen Walker, Carol A Folkes, Lisa K Stratford, Michael R Hayles, Jacqueline Hoe, Kwang-Lae Kim, Dong-Uk Park, Han-Oh Kearsey, Stephen E
...
Published in
Genes & development
Nucleotide synthesis is a universal response to DNA damage, but how this response facilitates DNA repair and cell survival is unclear. Here we establish a role for DNA damage-induced nucleotide synthesis in homologous recombination (HR) repair in fission yeast. Using a genetic screen, we found the Ddb1-Cul4(Cdt)² ubiquitin ligase complex and ribonu...
Munding, Elizabeth M Igel, A Haller Shiue, Lily Dorighi, Kristel M Treviño, Lisa R Ares, Manuel Jr
Published in
Genes & development
Splicing regulatory networks are essential components of eukaryotic gene expression programs, yet little is known about how they are integrated with transcriptional regulatory networks into coherent gene expression programs. Here we define the MER1 splicing regulatory network and examine its role in the gene expression program during meiosis in bud...
Vidal, S Khush, R S Leulier, F Tzou, P Nakamura, M Lemaitre, B
Published in
Genes & development
In mammals, TAK1, a MAPKKK kinase, is implicated in multiple signaling processes, including the regulation of NF-kappaB activity via the IL1-R/TLR pathways. TAK1 function has largely been studied in cultured cells, and its in vivo function is not fully understood. We have isolated null mutations in the Drosophila dTAK1 gene that encodes dTAK1, a ho...
Mizuno, Ken'Ichi Lambert, Sarah Murray, Johanne M Carr, Antony M
Published in
Genes & Development
Gene amplification plays important roles in the progression of cancer and contributes to acquired drug resistance during treatment. Amplification can initiate via dicentric palindromic chromosome production and subsequent breakage-fusion-bridge cycles. Here we show that, in fission yeast, acentric and dicentric palindromic chromosomes form by homol...
Miotto, Benoit Struhl, Kevin
Published in
Genes & Development
HBO1 histone acetylase is important for DNA replication licensing. In human cells, HBO1 associates with replication origins specifically during the G1 phase of the cell cycle in a manner that depends on the replication licensing factor Cdt1, but is independent of the Cdt1 repressor Geminin. HBO1 directly interacts with Cdt1, and it enhances Cdt1-de...
