Wei, Yu-Miao Li, Xiang Xu, Ming Abais, Justine M. Chen, Yang Riebling, Christopher R. Boini, Krishna M. Li, Pin-Lan Zhang, Yang
Published in
Cellular Physiology and Biochemistry
Background/Aims: In addition to their action of lowering blood cholesterol levels, statins modulate biological characteristics and functions of arterial myocytes such as viability, proliferation, apoptosis, survival and contraction. The present study tested whether simvastatin, as a prototype statin, enhances autophagy in coronary arterial myocytes...
Hill, Sarah J Rolland, Thomas Adelmant, Guillaume Xia, Xianfang Owen, Matthew S Dricot, Amélie Zack, Travis I Sahni, Nidhi Yves Jacob Hao, Tong
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Published in
Genes & Development
BRCA1 is a breast and ovarian tumor suppressor. Given its numerous incompletely understood functions and the possibility that more exist, we performed complementary systematic screens in search of new BRCA1 protein-interacting partners. New BRCA1 functions and/or a better understanding of existing ones were sought. Among the new interacting protein...
Gehman, Lauren T Meera, Pratap Stoilov, Peter Shiue, Lily O'Brien, Janelle E Meisler, Miriam H Ares, Manuel Jr Otis, Thomas S Black, Douglas L
Published in
Genes & development
The Rbfox proteins (Rbfox1, Rbfox2, and Rbfox3) regulate the alternative splicing of many important neuronal transcripts and have been implicated in a variety of neurological disorders. However, their roles in brain development and function are not well understood, in part due to redundancy in their activities. Here we show that, unlike Rbfox1 dele...
Nimonkar, Amitabh V Genschel, Jochen Kinoshita, Eri Polaczek, Piotr Campbell, Judith L Wyman, Claire Modrich, Paul Kowalczykowski, Stephen C
Published in
Genes & development
Repair of dsDNA breaks requires processing to produce 3'-terminated ssDNA. We biochemically reconstituted DNA end resection using purified human proteins: Bloom helicase (BLM); DNA2 helicase/nuclease; Exonuclease 1 (EXO1); the complex comprising MRE11, RAD50, and NBS1 (MRN); and Replication protein A (RPA). Resection occurs via two routes. In one, ...
Moss, Jennifer Tinline-Purvis, Helen Walker, Carol A Folkes, Lisa K Stratford, Michael R Hayles, Jacqueline Hoe, Kwang-Lae Kim, Dong-Uk Park, Han-Oh Kearsey, Stephen E
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Published in
Genes & development
Nucleotide synthesis is a universal response to DNA damage, but how this response facilitates DNA repair and cell survival is unclear. Here we establish a role for DNA damage-induced nucleotide synthesis in homologous recombination (HR) repair in fission yeast. Using a genetic screen, we found the Ddb1-Cul4(Cdt)² ubiquitin ligase complex and ribonu...
Munding, Elizabeth M Igel, A Haller Shiue, Lily Dorighi, Kristel M Treviño, Lisa R Ares, Manuel Jr
Published in
Genes & development
Splicing regulatory networks are essential components of eukaryotic gene expression programs, yet little is known about how they are integrated with transcriptional regulatory networks into coherent gene expression programs. Here we define the MER1 splicing regulatory network and examine its role in the gene expression program during meiosis in bud...
Vidal, S Khush, R S Leulier, F Tzou, P Nakamura, M Lemaitre, B
Published in
Genes & development
In mammals, TAK1, a MAPKKK kinase, is implicated in multiple signaling processes, including the regulation of NF-kappaB activity via the IL1-R/TLR pathways. TAK1 function has largely been studied in cultured cells, and its in vivo function is not fully understood. We have isolated null mutations in the Drosophila dTAK1 gene that encodes dTAK1, a ho...
Miotto, Benoit Struhl, Kevin
Published in
Genes & Development
HBO1 histone acetylase is important for DNA replication licensing. In human cells, HBO1 associates with replication origins specifically during the G1 phase of the cell cycle in a manner that depends on the replication licensing factor Cdt1, but is independent of the Cdt1 repressor Geminin. HBO1 directly interacts with Cdt1, and it enhances Cdt1-de...
Grallert, Agnes Krapp, Andrea Bagley, Steve Simanis, Viesturs Hagan, Iain M
Published in
Genes & development
Mitotic exit in Saccharomyces cerevisiae and septation in Schizosaccharomyces pombe are regulated by a conserved signaling network called the mitotic exit and septum initiation networks (SIN), respectively. The network is active on one of the two anaphase B spindle-pole bodies (SPBs). Whereas the inherent asymmetry of growth by budding accounts for...
Zuniga, Aimée Michos, Odyssé Spitz, François Haramis, Anna-Pavlina G Panman, Lia Galli, Antonella Vintersten, Kristina Klasen, Christian Mansfield, William Kuc, Sylwia
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Published in
Genes & development
The mouse limb deformity (ld) mutations cause limb malformations by disrupting epithelial-mesenchymal signaling between the polarizing region and the apical ectodermal ridge. Formin was proposed as the relevant gene because three of the five ld alleles disrupt its C-terminal domain. In contrast, our studies establish that the two other ld alleles d...
