Ochoa, Alexander Onorato, David P Fitak, Robert R Roelke-Parker, Melody E Culver, Melanie
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G3 (Bethesda, Md.)
In the mid-1990s, the population size of Florida panthers became so small that many individuals manifested traits associated with inbreeding depression (e.g., heart defects, cryptorchidism, high pathogen-parasite load). To mitigate these effects, pumas from Texas were introduced into South Florida to augment genetic variation in Florida panthers. I...
Cohn, Jesse Dwivedi, Vivek Valperga, Giulio Zarate, Nicole de Bono, Mario Horvitz, H Robert Pierce, Jonathan T
Published in
G3 (Bethesda, Md.)
The BH3-only family of proteins is key for initiating apoptosis in a variety of contexts, and may also contribute to non-apoptotic cellular processes. Historically, the nematode Caenorhabditis elegans has provided a powerful system for studying and identifying conserved regulators of BH3-only proteins. In C. elegans, the BH3-only protein egl-1 is e...
Spracklen, Andrew J Lamb, Maureen C Groen, Christopher M Tootle, Tina L
Published in
G3 (Bethesda, Md.)
Prostaglandins (PGs) are lipid signaling molecules with numerous physiologic functions, including pain/inflammation, fertility, and cancer. PGs are produced downstream of cyclooxygenase (COX) enzymes, the targets of non-steroidal anti-inflammatory drugs (NSAIDs). In numerous systems, PGs regulate actin cytoskeletal remodeling, however, their mechan...
Jin, Yuqing Zhao, Wei Nie, Shuai Liu, Si-Si El-Kassaby, Yousry A Wang, Xiao-Ru Mao, Jian-Feng
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G3 (Bethesda, Md.)
Platycladus orientalis is an ecologically important native conifer in Northern China and exotic species in many parts of the world; however, knowledge about the species' genetics and genome are very limited. The availability of well-developed battery of genetic markers, with large genome coverage, is a prerequisite for the species genetic dissectio...
Kasimatis, Katja R Ralph, Peter L Phillips, Patrick C
Published in
G3 (Bethesda, Md.)
Since the autosomal genome is shared between the sexes, sex-specific fitness optima present an evolutionary challenge. While sexually antagonistic selection might favor different alleles within females and males, segregation randomly reassorts alleles at autosomal loci between sexes each generation. This process of homogenization during transmissio...
Anderson, Sarah N Stitzer, Michelle C Zhou, Peng Ross-Ibarra, Jeffrey Hirsch, Cory D Springer, Nathan M
Published in
G3 (Bethesda, Md.)
Transposable Elements (TEs) are mobile elements that contribute the majority of DNA sequences in the maize genome. Due to their repetitive nature, genomic studies of TEs are complicated by the difficulty of properly attributing multi-mapped short reads to specific genomic loci. Here, we utilize a method to attribute RNA-seq reads to TE families rat...
Dong, Yan Li, Haimei Zhao, Liang Koopman, Peter Zhang, Feng Huang, Johnny X
Published in
G3 (Bethesda, Md.)
The emergence of the CRISPR-Cas9 system has triggered a technical revolution in mammalian genome editing. Compared to traditional gene-targeting strategies, CRISPR-Cas9 technology offers a more efficient and cost-effective approach for generating genetically modified animal models. However, off-target cleavage in CRISPR-mediated genome editing is a...
Liu, Boyang Sung, Hung-Wei Großhans, Jörg
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G3 (Bethesda, Md.)
Protein phosphatase V (PpV) encodes the Drosophila homolog of the evolutionarily conserved Protein Phosphatase 6 (PP6). The physiological and developmental functions of PpV/PP6 have not been well characterized due to lack of a genetically defined mutant. Here, we identified a PpV non-sense mutation and describe multiple mutant phenotypes in oogenes...
Ma, Huan Zhang, Lin Yu, Xinyang Wan, Yufeng Wang, Dongni Shi, Weirui Huang, Meiyan Xu, Manhao Shen, Enze Gao, Menghan
...
Published in
G3 (Bethesda, Md.)
Increasing evidence has pointed to the connection between pre-mRNA splicing and the circadian clock; however, the underlying mechanisms of this connection remain largely elusive. In the filamentous fungus Neurospora crassa, the core circadian clock elements comprise White Collar 1 (WC-1), WC-2 and FREQUENCY (FRQ), which form a negative feedback loo...
Stinchfield, Michael J Miyazawa, Keiji Newfeld, Stuart J
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G3 (Bethesda, Md.)
Uncovering how new members of multigene families acquire new functions is an important topic in evolutionary and developmental genetics. CORL proteins (SKOR in mice, Fussel in humans and fussel in Flybase) are a family of CNS specific proteins related to mammalian Sno/Ski oncogenes. Drosophila CORL (dCORL) participates in TGF-β and insulin signalin...
