Lepori, Vincent Mühlhause, Franziska Sewell, Adrian C Jagannathan, Vidhya Janzen, Nils Rosati, Marco Maximiano Alves de Sousa, Filipe Miguel Tschopp, Aurélie Schüpbach, Gertraud Matiasek, Kaspar
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Published in
G3 (Bethesda, Md.)
Several enzymes are involved in fatty acid oxidation, which is a key process in mitochondrial energy production. Inherited defects affecting any step of fatty acid oxidation can result in clinical disease. We present here an extended family of German Hunting Terriers with 10 dogs affected by clinical signs of exercise induced weakness, muscle pain,...
Ohkuni, Kentaro Levy-Myers, Reuben Warren, Jack Au, Wei-Chun Takahashi, Yoshimitsu Baker, Richard E Basrai, Munira A
Published in
G3 (Bethesda, Md.)
Stringent regulation of cellular levels of evolutionarily conserved centromeric histone H3 variant (CENP-A in humans, CID in flies, Cse4 in yeast) prevents its mislocalization to non-centromeric chromatin. Overexpression and mislocalization of CENP-A has been observed in cancers and leads to aneuploidy in yeast, flies, and human cells. Ubiquitin-me...
Runge, John S Raab, Jesse R Magnuson, Terry
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G3 (Bethesda, Md.)
Chromatin remodeling and histone modifying enzymes play a critical role in shaping the regulatory output of a cell. Although much is known about these classes of proteins, identifying the mechanisms by which they coordinate gene expression programs remains an exciting topic of investigation. One factor that may contribute to the targeting and activ...
Jaiswal, Deepika Jezek, Meagan Quijote, Jeremiah Lum, Joanna Choi, Grace Kulkarni, Rushmie Park, DoHwan Green, Erin M
Published in
G3 (Bethesda, Md.)
The conserved yeast histone methyltransferase Set1 targets H3 lysine 4 (H3K4) for mono, di, and trimethylation and is linked to active transcription due to the euchromatic distribution of these methyl marks and the recruitment of Set1 during transcription. However, loss of Set1 results in increased expression of multiple classes of genes, including...
Mogre, Aalap Veetil, Reshma T Seshasayee, Aswin Sai Narain
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G3 (Bethesda, Md.)
Evolve and resequence experiments have provided us a tool to understand bacterial adaptation to antibiotics. In our previous work, we used short-term evolution to isolate mutants resistant to the ribosome targeting antibiotic kanamycin, and reported that Escherichia coli develops low cost resistance to kanamycin via different point mutations in the...
Bhakta, Mehul S Gezan, Salvador A Clavijo Michelangeli, Jose A Carvalho, Melissa Zhang, Li Jones, James W Boote, Kenneth J Correll, Melanie J Beaver, James Osorno, Juan M
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Published in
G3 (Bethesda, Md.)
The common bean is a tropical facultative short-day legume that is now grown in tropical and temperate zones. This observation underscores how domestication and modern breeding can change the adaptive phenology of a species. A key adaptive trait is the optimal timing of the transition from the vegetative to the reproductive stage. This trait is res...
Whangbo, Jennifer S Weisman, Alexandra S Chae, Jeiwook Hunter, Craig P
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G3 (Bethesda, Md.)
In the nematode Caenorhabditis elegans, RNA interference (RNAi) triggered by double-stranded RNA (dsRNA) spreads systemically to cause gene silencing throughout the organism and its progeny. We confirm that Caenorhabditis nematode SID-1 orthologs have dsRNA transport activity and demonstrate that the SID-1 paralog CHUP-1 does not transport dsRNA. S...
Talla, Venkat Kalsoom, Faheema Shipilina, Daria Marova, Irina Backström, Niclas
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G3 (Bethesda, Md.)
Identification of candidate genes for trait variation in diverging lineages and characterization of mechanistic underpinnings of genome differentiation are key steps toward understanding the processes underlying the formation of new species. Hybrid zones provide a valuable resource for such investigations, since they allow us to study how genomes e...
Heringer, Pedro Dias, Guilherme B Kuhn, Gustavo C S
Published in
G3 (Bethesda, Md.)
Bracoviruses associate symbiotically with thousands of parasitoid wasp species in the family Braconidae, working as virulence gene vectors, and allowing the development of wasp larvae within hosts. These viruses are composed of multiple DNA circles that are packaged into infective particles, and injected together with wasp's eggs during parasitizat...
Xia, Xuhua
Published in
G3 (Bethesda, Md.)
Two major stumbling blocks exist in high-throughput sequencing (HTS) data analysis. The first is the sheer file size, typically in gigabytes when uncompressed, causing problems in storage, transmission, and analysis. However, these files do not need to be so large, and can be reduced without loss of information. Each HTS file, either in compressed ...
