Seabury, Christopher M Smith, Johanna L Wilson, Miranda L Bhattarai, Eric Santos, Jose E P Chebel, Ricardo C Galvão, Klibs N Schuenemann, Gustavo M Bicalho, Rodrigo C Gilbert, Rob O
...
Published in
G3 (Bethesda, Md.)
Subfertility represents one major challenge to enhancing dairy production and efficiency. Herein, we use a reproductive index (RI) expressing the predicted probability of pregnancy following artificial insemination (AI) with Illumina 778K genotypes to perform single and multi-locus genome-wide association analyses (GWAA) on 2,448 geographically div...
Cao, Xu Lenk, Guy M Meisler, Miriam H
Published in
G3 (Bethesda, Md.)
Loss-of-function mutations of FIG4 are responsible for neurological disorders in human and mouse that result from reduced abundance of the signaling lipid PI(3,5)P2. In contrast, loss-of-function mutations of the phosphoinositide kinase PIP4K2C result in elevated abundance of PI(3,5)P2. These opposing effects on PI(3,5)P2 suggested that we might be...
Elizondo, Elisa C Faircloth, Brant C Brumfield, Robb T Shakya, Subir B Ellis, Vincenzo A Schmidt, Carl J Kovach, Adrienne I Gregory Shriver, W
Published in
G3 (Bethesda, Md.)
The clapper rail (Rallus crepitans), of the family Rallidae, is a secretive marsh bird species that is adapted for high salinity habitats. They are very similar in appearance to the closely related king rail (R. elegans), but while king rails are limited primarily to freshwater marshes, clapper rails are highly adapted to tolerate salt marshes. Bot...
Sterrett, Maria C Farchi, Daniela Strassler, Sarah E Boise, Lawrence H Fasken, Milo B Corbett, Anita H
Published in
G3 (Bethesda, Md.)
The RNA exosome is a conserved molecular machine that processes/degrades numerous coding and non-coding RNAs. The 10-subunit complex is composed of three S1/KH cap subunits (human EXOSC2/3/1; yeast Rrp4/40/Csl4), a lower ring of six PH-like subunits (human EXOSC4/7/8/9/5/6; yeast Rrp41/42/43/45/46/Mtr3), and a singular 3'-5' exo/endonuclease DIS3/R...
Warsinger-Pepe, Natalie Chang, Carly Desroberts, Connor R Akbari, Omar S
Published in
G3 (Bethesda, Md.)
Heat-shock-inducible expression of genes through the use of heat-inducible promoters is commonly used in research despite leaky expression of downstream genes of interest without targeted induction (i.e. heat shock). The development of non-leaky inducible expression systems is of broad interest for both basic and applied studies, to precisely contr...
Jaime, Maria D L A Salem, Ghadi H Martinez, Daniel J Karott, Sean Flores, Alejandra Palmer, Cameron D Mahadevaraju, Sharvani Krynitsky, Jonathan Garmendia-Cedillos, Marcial Anderson, Sarah
...
Published in
G3 (Bethesda, Md.)
Non-mammalian model organisms have been essential for our understanding of the mechanisms that control development, disease, and physiology, but they are underutilized in pharmacological and toxicological phenotypic screening assays due to their low throughput in comparison with cell-based screens. To increase the utility of using Drosophila melano...
Bierman, Anandi Karsten, Minette Terblanche, John S
Published in
G3 (Bethesda, Md.)
The false codling moth (FCM; Thaumatotibia leucotreta, Meyrick; Lepidoptera: Tortricidae) is a highly polyphagous, major agricultural pest indigenous to sub-Saharan Africa. With growing international trade, there is an increasing concern about introducing this pest into other countries. In South Africa, FCM poses a risk to multiple crops, and is cu...
Song, Wan Podicheti, Ram Rusch, Douglas B Tracey, William Daniel
Published in
G3 (Bethesda, Md.)
Pseudouridine (Psi) is one of the most frequent post-transcriptional modification of RNA. Enzymatic Psi modification occurs on rRNA, snRNA, snoRNA, tRNA, and non-coding RNA and has recently been discovered on mRNA. Transcriptome-wide detection of Psi (Psi-seq) has yet to be performed for the widely studied model organism Drosophila melanogaster. He...
Peters Haugrud, Amanda R Zhang, Qijun Green, Andrew J Xu, Steven S Faris, Justin D
Published in
G3 (Bethesda, Md.)
Crop yield gains are needed to keep pace with a growing global population and decreasing resources to produce food. Cultivated emmer wheat is a progenitor of durum wheat and a useful source of genetic variation for trait improvement in durum. Here, we evaluated a recombinant inbred line population derived from a cross between the North Dakota durum...
Liu, Zhi-Wei Shi, Xing-Yun Duan, Shuang-Mei Nian, Bo Chen, Li-Jiao Zhang, Guang-Hui Lv, Cai-You Ma, Yan Zhao, Ming
Published in
G3 (Bethesda, Md.)
Flavonoids are rich in tea plants (Camellia sinensis), and responsible for the flavor and healthful benefits of tea beverage. The anthocyanin levels in the purple tender shoots are higher than in the general green leaves of tea plant, which provide special materials to search metabolic mechanisms of flavonoid enrichment in plant. In this work, flav...
