Real-time PCR for diagnosing and monitoring treatment effect of Strongyloides stercoralis infection in a non-endemic set...
Published in Frontiers in Parasitology
Real-time PCR for diagnosing and monitoring treatment effect of Strongyloides stercoralis infection in a non-endemic set...
Identification of biomarker candidates for filarial parasite infections by analysis of extracellular vesicles
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Published in Frontiers in Parasitology
Background Improved diagnostic tools are needed for detecting active filarial infections in humans. Tests are available that detect adult W. bancrofti circulating filarial antigen, but there are no sensitive and specific biomarker tests for brugian filariasis or loiasis. Here we explored whether extracellular vesicles released by filarial parasites...
Myeloid- and epithelial-derived RELMα contribute to tissue repair following lung helminth infection
Published in Frontiers in Parasitology
Soil-transmitted helminth (STH) infections impact billions of individuals globally; however, there is a need to clarify the long-term impacts of these infections on pulmonary health owing to their transient migration and subsequent damage to the lungs. In mouse models of these infections using Nippostrongylus brasiliensis, lung pathology persists a...
Immunomodulatory proteins from hookworms reduce cardiac inflammation and modulate regulatory responses in a mouse model ...
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Published in Frontiers in Parasitology
Introduction Hookworms are parasitic helminths that secrete a variety of proteins that induce anti-inflammatory immune responses, stimulating increased CD4+Foxp3+ regulatory T cells and IL-10 production. Hookworm-derived recombinant proteins AIP-1 and AIP-2 have been shown to reduce inflammation in mouse models of inflammatory bowel disease and inf...
Analysis of insecticides in long-lasting insecticidal nets using X-ray fluorescence spectroscopy and correlation with bi...
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Published in Frontiers in Parasitology
Background Long-lasting insecticidal nets (LLINs) are a key vector control tool used for the prevention of malaria. Active ingredient (AI) measurements in LLINs are essential for evaluating their quality and efficacy. The main aim of the present study was to determine the utility of X-ray fluorescence (XRF) spectroscopy as a suitable field-deployab...
Secreted and surface proteome and transcriptome of Opisthorchis felineus
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Published in Frontiers in Parasitology
Introduction Opisthorchis felineus, Opisthorchis viverrini, and Clonorchis sinensis are the most medically important species of fish-borne zoonotic trematodes. O. felineus is endemic to the river plains of Western Siberia and Eastern Europe, and it is estimated that more than 1.6 million people could be infected with this parasite. Chronic opisthor...
An end is in sight: a perspective on PCR as an endpoint for Chagas disease treatment trials
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Published in Frontiers in Parasitology
Novel therapies for chronic indeterminate Chagas disease (CICD) are needed, but trials are limited by the absence of tests to detect infection and early treatment efficacy. This perspective highlights the shortfalls and strengths of polymerase chain reaction (PCR) as a study endpoint for anti-parasitic drug development. Serologic reversion, the gol...
Macrophage metallothioneins participate in the antileishmanial activity of antimonials
Published in Frontiers in Parasitology
Host cell functions that participate in the pharmacokinetics and pharmacodynamics (PK/PD) of drugs against intracellular pathogen infections are critical for drug efficacy. In this study, we investigated whether macrophage mechanisms of xenobiotic detoxification contribute to the elimination of intracellular Leishmania upon exposure to pentavalent ...
Elucidating Spirocerca lupi spread in the Americas by using phylogenetic and phylogeographic analyses
Published in Frontiers in Parasitology
Spirocerca lupi is a parasitic nematode of domestic and wild canids of the world. This nematode induces esophageal spirocercosis and may eventually lead to carcinomas, aortic aneurisms, and death of the animal. Two genotypes of S. lupi have been described based on specimens from Europe, Asia, Africa, and Oceania, but no profound analysis has been c...
Unveiling challenges in real-time PCR strategies for detecting treatment failure: observations from clinical trials on c...
Published in Frontiers in Parasitology
Chagas disease (CD) caused by Trypanosoma cruzi remains a Neglected Tropical Disease with limited access to diagnosis and treatment, particularly for chronically infected patients. Clinical trials are underway to improve treatment using new drugs or different regimens, and Real-Time PCR is used to assess the parasitological response as a surrogate ...
