Laohakunakorn, Nadanai Grasemann, Laura Lavickova, Barbora Michielin, Grégoire Shahein, Amir Swank, Zoe Maerkl, Sebastian J.
Published in
Frontiers in Bioengineering and Biotechnology
Cell-free systems offer a promising approach to engineer biology since their open nature allows for well-controlled and characterized reaction conditions. In this review, we discuss the history and recent developments in engineering recombinant and crude extract systems, as well as breakthroughs in enabling technologies, that have facilitated incre...
Valle, Giacomo D’Anna, Edoardo Strauss, Ivo Clemente, Francesco Granata, Giuseppe Di Iorio, Riccardo Controzzi, Marco Stieglitz, Thomas Rossini, Paolo M. Petrini, Francesco M.
...
Published in
Frontiers in Bioengineering and Biotechnology
Recent experiments have shown that neural stimulation can successfully restore sensory feedback in upper-limb amputees improving their ability to control the prosthesis. However, the potential advantages of invasive sensory feedback with respect to non-invasive solutions have not been yet identified. Our hypothesis was that a difference would appea...
Poupart, Oriane Schmocker, Andreas Conti, Riccardo Moser, Christophe Nuss, Katja M. Grützmacher, Hansjörg Mosimann, Pascal J. Pioletti, Dominique P.
Published in
Frontiers in Bioengineering and Biotechnology
Intracranial aneurysms are increasingly being treated with endovascular therapy, namely coil embolization. Despite being minimally invasive, partial occlusion and recurrence are more frequent compared to open surgical clipping. Therefore, an alternative treatment is needed, ideally combining minimal invasiveness and long-term efficiency. Herein, we...
Gupta, Priyanka Pérez-Mancera, Pedro A. Kocher, Hemant Nisbet, Andrew Schettino, Giuseppe Velliou, Eirini G.
Published in
Frontiers in Bioengineering and Biotechnology
With a very low survival rate, pancreatic ductal adenocarcinoma (PDAC) is a deadly disease. This has been primarily attributed to (i) its late diagnosis and (ii) its high resistance to current treatment methods. The latter specifically requires the development of robust, realistic in vitro models of PDAC, capable of accurately mimicking the in vivo...
Gorte, Olga Hollenbach, Rebecca Papachristou, Ioannis Steinweg, Christian Silve, Aude Frey, Wolfgang Syldatk, Christoph Ochsenreither, Katrin
Published in
Frontiers in Bioengineering and Biotechnology
Single cell oil (SCO) produced by oleaginous yeasts is considered as a sustainable source for biodiesel and oleochemicals since its production does not compete with food or feed and high yields can be obtained from a wide variety of carbon sources, e.g., acetate or lignocellulose. Downstream processing is still costly preventing the broader applica...
Li, Mingyue Chen, Jiuzhou Wang, Yu Liu, Jiao Huang, Jingwen Chen, Ning Zheng, Ping Sun, Jibin
Published in
Frontiers in Bioengineering and Biotechnology
Corynebacterium glutamicum is an important workhorse for industrial production of diversiform bioproducts. Multiplex control of metabolic pathway genes is crucial for maximizing biosynthesis of desired products. However, few tools for simultaneously regulating multiple genes in C. glutamicum have been reported. Here, a CRISPR-dCpf1-based multiplex ...
Zhang, Caizhe Hong, Kui
Published in
Frontiers in Bioengineering and Biotechnology
Terpenoids are a large family of natural products with remarkable diverse biological functions, and have a wide range of applications as pharmaceuticals, flavors, pigments, and biofuels. Synthetic biology is presenting possibilities for sustainable and efficient production of high value-added terpenoids in engineered microbial cell factories, using...
Lee, Hyewon Baek, Ji In Kim, Su Jin Kwon, Kil Koang Rha, Eugene Yeom, Soo-Jin Kim, Haseong Lee, Dae-Hee Kim, Dong-Myung Lee, Seung-Goo
...
Published in
Frontiers in Bioengineering and Biotechnology
Methanotrophs with soluble methane monooxygenase (sMMO) show high potential for various ecological and biotechnological applications. Here, we developed a high throughput method to identify sMMO-producing microbes by integrating droplet microfluidics and a genetic circuit-based biosensor system. sMMO-producers and sensor cells were encapsulated in ...
Wang, Wei Lv, Hehe Zhao, Yuan Liu, Dong Wang, Yongqing Zhang, Yu
Published in
Frontiers in Bioengineering and Biotechnology
The studies on drug-protein interactions (DPIs) had significant for drug repositioning, drug discovery, and clinical medicine. The biochemical experimentation ( in vitro ) requires a long time and high cost to be confirmed because it is difficult to estimate. Therefore, a feasible solution is to predict DPIs efficiently with computers. We propose a...
Cheng, Zhongyi Xia, Yuanyuan Zhou, Zhemin
Published in
Frontiers in Bioengineering and Biotechnology
Nitrile hydratase (NHase, EC 4.2.1.84) is one type of metalloenzyme participating in the biotransformation of nitriles into amides. Given its catalytic specificity in amide production and eco-friendliness, NHase has overwhelmed its chemical counterpart during the past few decades. However, unclear catalytic mechanism, low thermostablity, and narrow...
