Published in Federation proceedings
Factors contributing to modifications in the capability for enzyme adaptation as an expression of aging are reviewed. Specific examples of altered enzyme adaptations during aging include the responses of hepatic glucokinase activity to glucose and hepatic tyrosine aminotransferase activity to starvation in Sprague-Dawley rats. These impaired enzyme...
Published in Federation proceedings
Cytochrome P-450 is the terminal oxidase of an electron transport system that is responsible for the oxidative metabolism of a large variety of endogenous and exogenous compounds. This broad substrate selectivity is caused by multiple isozymes of cytochrome P-450 and the wide substrate selectivity of many of these isozymes. We have isolated 11 isoz...
Published in Federation proceedings
Published in Federation proceedings
Complexity in a theoretical model may or may not be associated with a high level of arbitrariness, depending on how the model is constructed. In this paper I use examples from cardiac electrophysiology to illustrate two techniques for maintaining significance in complex simulations. First, the approaches of analysis and synthesis are compared as me...
Published in Federation proceedings
The transendothelial transfer of macromolecules has been difficult to study because of the complexities of the in vivo models. We have developed a model of an endothelium cultured on a permeable support and used it to characterize the transendothelial transfer of albumin. Porcine pulmonary artery endothelial cells form a single layer of cells linin...
Published in Federation proceedings
There is a twofold rationale for assaying acetylcholinesterase (AChE) (EC 3.1.1.7) immunologically, rather than by conventional activity-based methods: to measure the amount of enzyme protein in samples that may contain AChE of uncertain intrinsic activity; to bypass cumbersome procedures for determining the individual molecular forms of the enzyme...
Published in Federation proceedings
Scientific understanding in physics or physiology is based on models or theories devised to describe what is known, within the limits imposed by observation error. Carefully integrated models can be used for prediction, and the inferences assessed via further experiments designed to test the adequacy of the theory summarizing the state of knowledge...
Published in Federation proceedings
The dynamics of complex systems can be effectively analyzed by judicious use of intrinsic time constants. Order of magnitude estimation based on time constants has been used successfully to examine the dynamic behavior of complicated processes. The main goal of this paper is to introduce this approach to the analysis of complex metabolic systems. T...
Published in Federation proceedings
The observations in an experiment define a set of observational parameters that are functions of the basic kinetic parameters of the model of the system. The problem of identifiability is concerned with whether the observational parameters uniquely specify the basic kinetic parameters. As such, it depends only on the functional relation between the...
Published in Federation proceedings
A comprehensive synthetic approach, previously developed in this laboratory, has been applied to systematically screen the entire extracellular part (residues 1-210) of the alpha chain of the Torpedo californica acetylcholine receptor (AChR) for the profiles of the continuous regions that are recognized by antibodies against free, or membrane-seque...
