Krocová, Eliška Neradová, Sylva Kupcik, Rudolf Janovská, Sylva Bílková, Zuzana Heidingsfeld, Olga
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FEMS yeast research
Most of the phosphatases of human fungal pathogens Candida albicans and C. parapsilosis have never been experimentally characterised, although dephosphorylation reactions are central to many biological processes. PHO15 genes of these yeasts have been annotated as the sequences encoding 4-nitrophenyl phosphatase, on the basis of homology to PHO13 ge...
Kumar, Deepak Sharma, Nimisha
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FEMS yeast research
Transcription of protein-coding genes is a highly regulated process. In eukaryotes, it involves cross-talk between the RNA polymerase II enzyme and different proteins of the transcriptional machinery. Twelve different subunits, Rpb1 to Rpb12, constitute RNA polymerase II. The sequence of the Rpb7 subunit is highly conserved across organisms. Howeve...
Giannattasio, Sergio Mazzoni, Cristina
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FEMS yeast research
Cioch-Skoneczny, Monika Satora, Pawel Skotniczny, Magdalena Skoneczny, Szymon
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FEMS yeast research
The aim of the study was to characterise, quantitatively and qualitatively, the yeast microbiota found during spontaneous fermentation of grape musts obtained from the red grape varieties 'Rondo' and 'Regent'. For the research, grapes originating from two vineyards (Srebrna Góra and Spotkaniówka) located in southern Poland were used. Musts of the s...
Knoblach, Barbara Rachubinski, Richard A
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FEMS yeast research
We report the permanent introduction of the human peroxisomal β-oxidation enzymatic machinery required for straight chain degradation of fatty acids into the yeast, Saccharomyces cerevisiae. Peroxisomal β-oxidation encompasses four sequential reactions that are confined to three enzymes. The genes encoding human acyl-CoA oxidase 1, peroxisomal mult...
Tulha, Joana Lucas, Cândida
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FEMS yeast research
Gup1 is the yeast counterpart of the high eukaryotes HHATL. This and the close homologue Gup2/HHAT regulate the Hedgehog morphogenic, developmental pathway. In yeasts, a similar paracrine pathway is not known though the Δgup1 mutant is associated with morphology and proliferation/death processes. As a first step toward identifying the actual molecu...
Mbuyane, Lethiwe L de Kock, Marli Bauer, Florian F Divol, Benoit
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FEMS yeast research
Non-Saccharomyces yeasts impact wine fermentations and can diversify the flavor profiles of wines. However, little information is available on the metabolic networks of most of these species. Here we show that unlike the main wine yeast Saccharomyces cerevisiae, Torulaspora delbrueckii and to a lesser extent Lachancea thermotolerans produce signifi...
Ghosh, Rupam Dong, Jijun Wall, Joe Frederick, Kendra K
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FEMS yeast research
Yeast prions are self-templating protein-based mechanisms of inheritance whose conformational changes lead to the acquisition of diverse new phenotypes. The best studied of these is the prion domain (NM) of Sup35, which forms an amyloid that can adopt several distinct conformations (strains) that confer distinct phenotypes when introduced into cell...
Tariq, Amber Lin, JiaBei Noll, Megan M Torrente, Mariana P Mack, Korrie L Murillo, Oscar Hernandez Jackrel, Meredith E Shorter, James
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FEMS yeast research
Hsp104 is a hexameric AAA + ATPase and protein disaggregase found in yeast, which can be potentiated via mutations in its middle domain (MD) to counter toxic phase separation by TDP-43, FUS and α-synuclein connected to devastating neurodegenerative disorders. Subtle missense mutations in the Hsp104 MD can enhance activity, indicating that post-tran...
Veri, Amanda O Robbins, Nicole Cowen, Leah E
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FEMS yeast research
The impact of fungal pathogens on human health is devastating. For fungi and other pathogens, a key determinant of virulence is the capacity to thrive at host temperatures, with elevated temperature in the form of fever as a ubiquitous host response to defend against infection. A prominent feature of cells experiencing heat stress is the increased ...
