Arras, Samantha D M Hibbard, Taylor R Mitsugi-McHattie, Lucy Woods, Matthew A Johnson, Charlotte E Munkacsi, Andrew Denmat, Sylvie Hermann-Le Ganley, Austen R D
Published in
FEMS yeast research
Saccharomyces cerevisiae is an exceptional genetic system, with genetic crosses facilitated by its ability to be maintained in haploid and diploid forms. Such crosses are straightforward if the mating type/ploidy of the strains are known. Several techniques can determine mating type (or ploidy), but all have limitations. Here we validate a simple, ...
Ata, Özge Ergün, Burcu Gündüz Fickers, Patrick Heistinger, Lina Mattanovich, Diethard Rebnegger, Corinna Gasser, Brigitte
Published in
FEMS yeast research
The important industrial protein production host Komagataella phaffii (syn Pichia pastoris) is classified as a non-conventional yeast. But what exactly makes K. phaffii non-conventional? In this review we set out to address the main differences to the 'conventional' yeast Saccharomyces cerevisiae, but also pinpoint differences to other non-conventi...
Solieri, Lisa Cassanelli, Stefano Huff, Franziska Barroso, Liliane Branduardi, Paola Louis, Edward J Morrissey, John P
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FEMS yeast research
Evolution has provided a vast diversity of yeasts that play fundamental roles in nature and society. This diversity is not limited to genotypically homogenous species with natural interspecies hybrids and allodiploids that blur species boundaries frequently isolated. Thus, life-cycle and the nature of breeding systems have profound effects on genom...
Perli, Thomas Borodina, Irina Daran, Jean-Marc
Published in
FEMS yeast research
Engineering a new metabolic function in a microbial host can be limited by the availability of the relevant cofactor. For instance, in Yarrowia lipolytica, the expression of a functional nitrate reductase is precluded by the absence of molybdenum cofactor (Moco) biosynthesis. In this study, we demonstrated that the Ogataea parapolymorpha Moco biosy...
Godinho, Cláudia P Palma, Margarida Oliveira, Jorge Mota, Marta N Antunes, Miguel Teixeira, Miguel C Monteiro, Pedro T Sá-Correia, Isabel
Published in
FEMS yeast research
Responding to the recent interest of the yeast research community in non-Saccharomyces cerevisiae species of biotechnological relevance, the N.C.Yeastract (http://yeastract-plus.org/ncyeastract/) was associated to YEASTRACT + (http://yeastract-plus.org/). The YEASTRACT + portal is a curated repository of known regulatory associations between transc...
Ohtsuka, Hokuto Shimasaki, Takafumi Aiba, Hirofumi
Published in
FEMS yeast research
Sulfur is an essential component of various biologically important molecules, including methionine, cysteine and glutathione, and it is also involved in coping with oxidative and heavy metal stress. Studies using model organisms, including budding yeast (Saccharomyces cerevisiae) and fission yeast (Schizosaccharomyces pombe), have contributed not o...
Feng, Weijia Yang, Mengdi Li, Xin Wei, Dongsheng
Published in
FEMS yeast research
ATG8 is one of the critical genes that participate in several essential autophagic steps. The expression of ATG8 must be exquisitely regulated to avoid physiological disorder and even cell death. However, the mechanisms of regulating ATG8 expression remain to be fully uncovered. In this investigation, we found that Dicer homologs in Cryptococcus ne...
Sestric, Ryan Spicer, Vic Krokhin, Oleg V Sparling, Richard Levin, David B
Published in
FEMS yeast research
Oleaginous yeasts have the ability to store greater than 20% of their mass as neutral lipids, in the form of triacylglycerides. The ATP citrate lyase is thought to play a key role in triacylglyceride synthesis, but the relationship between expression levels of this and other related enzymes is not well understood in the role of total lipid accumula...
Li, Chenxi Hou, Shaonan Ma, Xiaoyu Li, Jiajia Huo, Liang Zhang, Ping Hao, Xiaoran Zhu, Xudong
Published in
FEMS yeast research
Epigenetic marks or post-translational modifications on histones have important regulatory roles in gene expression in eukaryotic organisms. The epigenetic regulation of gene expression in the pathogenic yeast Cryptococcus deneoformans remains largely undetermined. The YEATS domain proteins are readers of crotonylated lysine residues in histones. H...
Domenzain, Iván Li, Feiran Kerkhoven, Eduard J Siewers, Verena
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FEMS yeast research
Metabolic network reconstructions have become an important tool for probing cellular metabolism in the field of systems biology. They are used as tools for quantitative prediction but also as scaffolds for further knowledge contextualization. The yeast Saccharomyces cerevisiae was one of the first organisms for which a genome-scale metabolic model ...
