Published in Experimental Eye Research
The purpose of this study was to evaluate the expression of the SARS-CoV-2 receptors ACE2 and TMPRSS2 in an immortalized human conjunctival epithelial cell line and in healthy human conjunctiva excised during ocular surgery, using Western blot, confocal microscopy and immunohistochemistry. The Western blot showed that ACE2 and TMPRSS2 proteins were...
Published in Experimental Eye Research
The angiotensin-converting enzyme 2 ( ACE2 ) receptor has been proved for SARS-CoV-2 cell entry after auxiliary cellular protease priming by transmembrane protease serine 2 ( TMPRSS2 ), but the co-effect of this molecular mechanism was unknown. Here, single-cell sequencing was performed with human conjunctiva and the results have shown that ACE2 an...
Published in Experimental Eye Research
• Severe Covid-19 infection induces multiple, massive immune reactions in humans. • This research study evaluated potential immune cross-reactivity sequences/targets in the eye. • Bioinformatics was used to detect sequence similarities for ocular autoimmunity after Covid-19. • No significant protein sequence similarity was found between five SARS-C...
Published in Experimental Eye Research
• Analyses of 16 plasma trace element levels in neovascular AMD patients vs controls. • Barium and cadmium are elevated in 236 cases compared to 236 age-matched controls. • Chromium levels are reduced in the neovascular AMD patients compared to controls. • Environmental and lifestyle factors affect trace-element levels in neovascular AMD. • Trace e...
In piglets we tested the applicability of digital video microscopy and diffuse reflectance spectroscopy for non-invasive assessments of limbal and bulbar conjunctival microcirculation. A priori we postulated that the metabolic rate is higher in limbal as compared to bulbar conjunctiva, and that this difference is reflected in micro vascular structu...
Published in Experimental Eye Research
• Manifestation of SARS-CoV-2 in the intraocular milieu. • Manifestation of SARS-CoV-2 in the vitreous. • Manifestation of SARS-CoV-2 in the aqueous humor.
Published in Experimental Eye Research
• Hyperinduction of proinflammatory cytokine plays an important role in the disease progression of patients infected with SARS-CoV-2. • Tear levels of cytokines are increased in COVID-19 patients.
Published in Experimental Eye Research
• mgΔloxPneo mice showed abnormalities in corneal thickness from embryonic development through to adulthood. • Elastic fibres were evident from E16.5 in both the WT and mgΔloxPneo mouse corneas. • Adult mgΔloxPneo mouse corneas exhibited a disorganised elastic fibre network with unusually high levels of branching. • The disrupted collagen arrangeme...
Published in Experimental Eye Research
• PRPF31 is the second most common cause of autosomal dominant retinitis pigmentosa and a potential target for gene therapy. • We present all reported pathogenic variants in PRPF31 as a resource for clinicians, diagnostic genetics labs, and researchers. • Genotype-phenotype correlations suggest that, dominant negative effects contribute to disease ...
Published in Experimental Eye Research
• Active inflammation continues in cases of persistent uveitis. • Some patients develop ectopic lymphoid-like structure. • In these cases targeting B cells may be beneficial.
