Rosani, Umberto del Vecchio, Claudia Franchin, Elisa Brun, Paola Ferrari, Stefano Ponzin, Diego Leonardi, Andrea
Published in
Experimental Eye Research
COVID-19's impact on the ocular surface has already been recognized, however the molecular mechanisms induced by the infection on the ocular surface are still unclear. The aim of this paper is to provide a first overview of the transcriptional perturbations caused by SARS-CoV-2 on the ocular surface by analyzing gene expression profile of corneoscl...
Klaska, Izabela P. White, Anne Villacampa, Pilar Hoke, Justin Abelleira-Hervas, Laura Maswood, Ryea N. Ali, Robin R. Bunce, Catey Unwin, Richard D. Cooper, Garth J.S.
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Published in
Experimental Eye Research
• Human recombinant opticin protects against the development of pathological angiogenesis in mice. • Human recombinant opticin accelerates regression of established pre-retinal neovascularization in mice. • Intraocular administration of recombinant human opticin offers the potential for a safe and effective therapy for neovascular retinal diseases....
Shivshetty, Nagaveni Swift, Thomas Pinnock, Abigail Pownall, David Neil, Sheila Mac Douglas, Ian Garg, Prashant Rimmer, Stephen
Published in
Experimental eye research
Corneal ulcers, a leading cause of blindness in the developing world are treated inappropriately without prior microbiology assessment because of issues related to availability or cost of accessing these services. In this work we aimed to develop a device for identifying the presence of Gram-positive or Gram-negative bacteria or fungi that can be u...
Ching, Jared Osborne, Andrew Eva, Richard Prudent, Julien Yu-Wai-Man, Patrick
Published in
Experimental Eye Research
Membrane contact sites (MCS) play crucial roles in cell physiology with dysfunction in several MCS proteins being linked with neurological and optic nerve diseases. Although there have been significant advances in imaging these interactions over the past two decades with advanced electron microscopy techniques, super-resolution imaging and proximit...
Yao, Yao Ma, Di Xu, Yanxuan Yuan, Xiang-Ling Liang, Jia-Jian Chen, Shao-Lang Jhanji, Vishal Ng, Tsz Kin
Published in
Experimental eye research
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the pathogen for coronavirus disease 2019 (COVID-19) pandemic. Its infection depends on the binding of spike protein to the host cell receptor angiotensin-converting enzyme 2 (ACE2), type II transmembrane serine protease (TMPRSS2) and neuropilin-1 (NRP1). Hydroxychloroquine has been ap...
Gilhooley, Michael J. Hickey, Doron G. Lindner, Moritz Palumaa, Teele Hughes, Steven Peirson, Stuart N. MacLaren, Robert E. Hankins, Mark W.
Published in
Experimental Eye Research
• Bipolar cells are attractive targets for therapeutic optogenetics in IRDs. • This is the first cell specific transcriptomic analysis of bipolar cells in an IRD model. • Bipolar cells maintain expression of genes essential to act as targets for optogenetics. • Protein staining relating to four candidate genes (Anxa7, Cntn1, Srm2, Sulf2) is confirm...
Ma, Ruiqi Gan, Lu Jiang, Sanjie Ding, Peiwen Chen, Dongsheng Wu, Jihong Qian, Jiang
Published in
Experimental eye research
The angiotensin-converting enzyme 2 (ACE2) receptor has been proved for SARS-CoV-2 cell entry after auxiliary cellular protease priming by transmembrane protease serine 2 (TMPRSS2), but the co-effect of this molecular mechanism was unknown. Here, single-cell sequencing was performed with human conjunctiva and the results have shown that ACE2 and TM...
Mencucci, Rita Favuzza, Eleonora Becatti, Matteo Tani, Alessia Mazzantini, Costanza Vignapiano, Roberto Fiorillo, Claudia Pellegrini-Giampietro, DomenicoE Manetti, Mirko Marini, Mirca
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Published in
Experimental eye research
The purpose of this study was to evaluate the expression of the SARS-CoV-2 receptors ACE2 and TMPRSS2 in an immortalized human conjunctival epithelial cell line and in healthy human conjunctiva excised during ocular surgery, using Western blot, confocal microscopy and immunohistochemistry. The Western blot showed that ACE2 and TMPRSS2 proteins were...
Karagöz, Işıl Kutlutürk Munk, Marion R. Kaya, Mücahit Rückert, René Yıldırım, Mustafa Karabaş, Levent
Published in
Experimental Eye Research
• Severe Covid-19 infection induces multiple, massive immune reactions in humans. • This research study evaluated potential immune cross-reactivity sequences/targets in the eye. • Bioinformatics was used to detect sequence similarities for ocular autoimmunity after Covid-19. • No significant protein sequence similarity was found between five SARS-C...
List, Wolfgang Regitnig, Peter Kashofer, Karl Gorkiewicz, Gregor Zacharias, Martin Wedrich, Andreas Posch-Pertl, Laura
Published in
Experimental eye research
The purpose of this research is to study the intraocular occurrence of SARS-CoV-2. In postmortem examinations, aqueous humor and the vitreous samples were collected. All individuals were previously positive in nasopharyngeal swabbing and cause of death was respiratory failure due to SARS-CoV-2 infection. Testing was done using quantitative RT-PCR. ...
