Published in European journal of immunology
Replication-incompetent adenovirus (Ad) vector and mRNA-lipid nanoparticle (LNP) constructs represent two modular vaccine platforms that have attracted substantial interest over the past two decades. Due to the COVID-19 pandemic and the rapid development of multiple successful vaccines based on these technologies, there is now clear real-world evid...
Published in European journal of immunology
Foxp3+ T regulatory (Treg) cells suppress inflammation and are essential for maintaining tissue homeostasis. A growing appreciation of tissue-specific Treg functions has built interest in leveraging the endogenous suppressive mechanisms of these cells into cellular therapeutics in organ-specific diseases. Notably, Treg cells play a critical role in...
Published in European journal of immunology
Adipose tissue inflammation is a driving factor for the development of obesity-associated metabolic disturbances, and a role of adipose tissue T cells in initiating the pro-inflammatory signaling is emerging. However, data on human adipose tissue T cells in obesity are limited, reflected by the lack of phenotypic markers to define tissue-resident T...
Published in European Journal of Immunology
Adaptive immunity strongly relies on the clonal expansion of antigen‐specific CD4+ T cells following challenge. We found that neutrophils decisively contributed to this amplification, and that their inhibition via TLR9 signaling compensated for the lack of regulatory T or B cells in autoimmune disease, underlining the quantitative importance of thi...
Published in European journal of immunology
Memory CD8+ T cells are indispensable for maintaining long-term immunity against intracellular pathogens and tumors. Despite their presence at oxygen-deprived infected tissue sites or in tumors, the impact of local oxygen pressure on memory CD8+ T cells remains largely unclear. We sought to elucidate how oxygen pressure impacts memory CD8+ T cells ...
Published in European journal of immunology
Type 1 Innate Lymphoid cells (ILC1s) are tissue-resident cells that partake in the regulation of inflammation and homeostasis. A major feature of ILC1s is their ability to rapidly respond after infections. The effector repertoire of ILC1s includes the pro-inflammatory cytokines IFN-γ and TNF-α and cytotoxic mediators such as granzymes, which enable...
Published in European Journal of Immunology
An in‐depth T cell receptor Vβ repertoire analysis of human blood CD4+ T helper cells specific for different microbial antigens (i.e. influenza vaccine, tetanus toxoid, and Candida albicans ) revealed that circulating T follicular helper cells are heterogeneous in phenotype and persistence.
Published in European Journal of Immunology
We show that SLPI, an inhibitor of NF‐κB, is expressed by human THP‐1 monocytic cells and by CD68‐expressing cells in the human intestinal lamina propria. We demonstrate that endogenous SLPI inhibits NF‐κB activation and subsequent CXCL8 and TNF‐α production in human THP‐1 monocytic cells in response to microbial stimulation.
Published in European journal of immunology
The hallmark of DCs is their potent and outstanding capacity to activate naive resting T cells. As such, DCs are the sentinels of the immune system and instrumental for the induction of immune responses. This is one of the reasons, why DCs became the focus of immunotherapeutical strategies to fight infections, cancer, and autoimmunity. Besides the ...
Published in European journal of immunology
This novel 26-color flow cytometry panel allows the detailed immune phenotyping of the complex network of myeloid cells in murine lymph nodes and skin. With the optimized panel the different murine DC-subsets and other myeloid cell types can be identified and further characterized for co-stimulatory and inhibitory surface molecules. © 2022 The Auth...
