Published in European Journal of Immunology
Luciferase IP system (LIPS) assay is a sensitive method for quantitative detection of antibodies to antigens in their native conformation. We here describe LIPS method to detect antibody responses to SARS‐CoV‐2 spike (S) and nucleocapsid (N) proteins in COVID‐19 patients.
Published in European Journal of Immunology
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Published in European Journal of Immunology
The recent outbreak of coronavirus disease 2019 (COVID‐19), triggered by the severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) poses an enormous threat to global public health and economies. Human coronaviruses normally cause no or mild respiratory disease but in the past two decades, potentially fatal coronavirus infections have emerged...
Published in European Journal of Immunology
Airway epithelial cells actively contribute to protection of the respiratory tract, by locally secreting soluble pattern recognition receptors (sPRR). The sPRR play critical roles in the recognition of conserved motifs and are linked to complement activation. This article reviews the current knowledge on the involvement of sPRRs in the pathogenesis...
Published in European Journal of Immunology
The protein production of IFN‐γ in T cells is regulated through AU‐rich elements (AREs) that are present in the 3′ untranslated region of the IFNG mRNA. We show here that the role of AREs to regulate IFN‐γ protein production is conserved in primary human T cells. Genetic removal of AREs by CRISPR‐Cas9 technology increased mRNA stability and resulte...
Published in European Journal of Immunology
Gut microbiota alteration due to antibiotic treatment is able to affect glioma growth in mice. Cytotoxic NK cell subsets and microglia functions are early impaired after antibiotics paving the way to glioma growth. The present study shows for the first time the presence of a functional gut–immune–brain tumor axis.
Published in European Journal of Immunology
Published in European Journal of Immunology
NKG2D has a dual function in NK cell cytotoxicity; while NKG2D is a crucial trigger for cytotoxicity against tumor cells expressing activating ligands, it limits perforin production in IL‐2 activated NK cells. The increased perforin levels are not linked to an altered activation of the JAK/STAT pathway.
Published in European Journal of Immunology
Triggering some TNF receptor superfamily (TNFRSF) members, such as TNFR2, 4‐1BB, GITR or DR3, on purified Foxp3+ regulatory T cells (Treg) induces activation of common signaling pathways, including canonical NF‐κB. This leads to increased proliferation, survival, and suppressive function of Treg, enhancing their capacity to suppress colitis.
Published in European Journal of Immunology
Unbiased flow cytometry profiling in non‐Hodgkin orbital lymphoma and idiopathic orbital inflammation identified and independently replicated diminished pDC and cDC2 populations in blood and tissue.
