Mouton, William Compagnon, Christelle Saker, Kahina Daniel, Soizic Djebali, Sophia Lacoux, Xavier Pozzetto, Bruno Oriol, Guy Laubreton, Daphné Prieux, Margaux
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Published in
European Journal of Immunology
Antigen‐specific T‐cells are essential for protective immunity against SARS‐CoV‐2. We set up a semi‐automated whole‐blood Interferon‐gamma release assay (WB IGRA) to monitor the T‐cell response after stimulation with SARS‐CoV‐2 peptide pools. We report that the WB IGRA is complementary to serological assays to assess SARS‐CoV‐2 immunity.
Kremer, Andreas E. Kremer, Anita N. Willam, Carsten Völkl, Simon Verhagen, Johan Achenbach, Susanne van der Meijden, Edith D. Lang, Vanessa Aigner, Michael Maier, Clara
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Published in
European Journal of Immunology
Treatment of B‐cell depleted patients suffering from COVID‐19 with convalescent plasma led to viral clearance and was followed by an increase in peptide‐specific CD4+ T‐cells suggesting that transient antibody titers promoted specific cellular immunity in these patients.
Cáceres‐Martell, Yaiza Fernández‐Soto, Daniel Campos‐Silva, Carmen García‐Cuesta, Eva M. Casasnovas, Jose M Navas‐Herrera, David Beneítez‐Martínez, Alexandra Martínez‐Fleta, Pedro Alfranca, Arantzazu Sánchez‐Madrid, Francisco
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Published in
European Journal of Immunology
Magnetic fluorescent beads coated with different SARS‐CoV‐2 antigens can be mixed to test simultaneously for antibodies present in patient plasma in highly multiplexed flow cytometry‐based assays. This sensitive method allows identification, in a single reaction, of several Ig‐isotypes and specificities, revealing differential responses between COV...
Peter, Antonia Sophia Roth, Edith Schulz, Sebastian R. Fraedrich, Kirsten Steinmetz, Tobit Damm, Dominik Hauke, Manuela Richel, Elie Mueller‐Schmucker, Sandra Habenicht, Katharina
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Published in
European Journal of Immunology
TRIANNI mice carry an entire set of human immunoglobulin V region gene segments and are a powerful tool to rapidly isolate human monoclonal antibodies. After immunizing these mice with DNA encoding the spike protein of SARS‐CoV‐2 and boosting with spike protein, we identified 29 hybridoma antibodies that reacted with the SARS‐CoV‐2 spike protein. N...
Marongiu, Laura Protti, Giulia Facchini, Fabio A. Valache, Mihai Mingozzi, Francesca Ranzani, Valeria Putignano, Anna Rita Salviati, Lorenzo Bevilacqua, Valeria Curti, Serena
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Published in
European Journal of Immunology
Growing evidence suggests that conventional dendritic cells (cDCs) undergo aberrant maturation in COVID‐19, which negatively affects T‐cell activation. The presence of effector T cells in patients with mild disease and dysfunctional T cells in severely ill patients suggests that adequate T‐cell responses limit disease severity. Understanding how cD...
Tuganbaev, Timur Honda, Kenya
Published in
European Journal of Immunology
Immune circuits of microbiome perception and response mediated by gut innate and adaptive immune systems, epithelial cells, and enteric nervous system have evolved to protect not only the host, but commensal microbiome as well. The immune curatorship together with the laws of microbial ecology defines the healthy microbiome.
Jurgens, Anouk P. Popović, Branka Wolkers, Monika C.
Published in
European Journal of Immunology
T cells continuously receive signals for protein production. Although quiescent T cells produce selected proteins needed for homeostasis, activated T cells undergo substantial proteome remodeling when activated through the TCR. Here, we describe how post‐transcriptional mechanisms define the protein production in T cells, with a focus on RNA‐bindin...
Yuan, Xiaodong Rasul, Faiz Nashan, Björn Sun, Cheng
Published in
European Journal of Immunology
Innate lymphoid cells (ILCs) have been documenting as emerging players associated with tumor progression and inhibition. Therefore, understanding the underlying mechanisms of ILCs in shaping the immune response in tumor could be in favor of developing approaches against tumors.
Campana, Stefania De Pasquale, Claudia Barberi, Chiara Oliveri, Daniela Sidoti Migliore, Giacomo Galletti, Bruno Guarneri, Claudio Cannavò, Serafinella Patrizia Ferlazzo, Guido
Published in
European journal of immunology
Human CD117+ CRTH2neg innate lymphoid cells (ILC) comprise multipotent precursors (ILCp), which are able to differentiate into subtypes in response to different signals received in peripheral tissues. NKp46+ ILCp have been reported to associate with ILC3 whereas KLRG1+ ILCp with ILC2, although the latter can also generate other ILC subsets, thus, m...
De Zuani, Marco Hortová-Kohoutková, Marcela Andrejčinová, Ivana Tomášková, Veronika Šrámek, Vladimír Helán, Martin Frič, Jan
Published in
European journal of immunology
Myeloid-derived suppressor cells (MDSCs) are important regulators of immune processes during sepsis in mice. However, confirming these observations in humans has been challenging due to the lack of defined preparation protocols and phenotyping schemes for MDSC subsets. Thus, it remains unclear how MDSCs are involved in acute sepsis and whether they...
